Carbohydrate analysis: From sample preparation to <scp>HPLC</scp> on different stationary phases coupled with evaporative light‐scattering detection

Dvořáčková, Eva; Šnóblová, Marie; Hrdlička, P.

doi:10.1002/jssc.201301089

Cited by 51 publications

(35 citation statements)

References 87 publications

(116 reference statements)

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“…(), and HPLC‐ELSD conditions described by Shanmugavelan et al . () with some modifications (Sanz & Martínez‐Castro, ; Dvořáčková et al ., ). Each lupin sample (0.1 g) was mixed with 4 mL of ultrapure water and shaken in a shaking water bath (SWB20; Ratek, Melbourne, Vic, Australia) at 50 °C for 1 hr.…”

Section: Methodsmentioning

confidence: 99%

Effect of cultivar, cultivation year and dehulling on raffinose family oligosaccharides in Australian sweet lupin (Lupinus angustifolius L.)

Karnpanit

Coorey

Clements

et al. 2016

Int J of Food Sci Tech

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Summary The aim of this study was to determine the effect of cultivar, cultivation year and dehulling on raffinose family oligosaccharides (RFOs) in current cultivars of Australian sweet lupin. Seed samples of ten cultivars grown in 2011, 2012 and 2013 were used in the study. Both whole seed and dehulled lupin samples were analysed for RFOs by high‐performance liquid chromatography with evaporative light scattering detector. Lupin cultivar had a significant effect on RFO contents. Total RFO contents in whole seed and dehulled lupin samples varied between 7.3–10.1 g/100 g DM and 7.6–16.8 g/100 g DM, respectively. Belara and Mandelup cultivars had high levels of RFOs indicating the suitability for functional foods with prebiotic effect. Gungurru and PBA Barlock contained low levels of RFOs and recommended for lupin‐enriched foods with low flatulence effect. Cultivation year with similar climatic conditions had no significant effect on RFO contents. Dehulling increases raffinose, stachyose and total RFO contents in lupin.

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Section: Methodsmentioning

confidence: 99%

Effect of cultivar, cultivation year and dehulling on raffinose family oligosaccharides in Australian sweet lupin (Lupinus angustifolius L.)

Karnpanit

Coorey

Clements

et al. 2016

Int J of Food Sci Tech

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“…For HPLC, the conventional fluorescence detection of underivatized carbohydrates is not applicable since their molecules do not contain fluorophores and UV detection must be performed at very low wavelength where many interferences may occur. For this reason, either refractive index, evaporative light scattering , pulsed amperometric , or mass spectrometric (MS or MS/MS) ] detection are utilized. The separation on pellicular CarboPac series of columns specifically designed for carbohydrate anion exchange separations has been proven adequate for the analysis of a wide range of carbohydrates (including sugar alcohols and larger polysaccharides).…”

Section: Introductionmentioning

confidence: 99%

“…Such methods are available as books [2,3], company application notes, and user guides [4][5][6][7][8], and numerous papers published in peer reviewed journals (e.g. [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26]). Carbohydrates (the inositols also can be considered carbohydrates having the formula C 6 H 12 O 6 ) have very polar molecules and are not volatile compounds such that GC analysis is not possible without analyte derivatization.…”

Section: Introductionmentioning

confidence: 99%

Analysis of small carbohydrates in several bioactive botanicals by gas chromatography with mass spectrometry and liquid chromatography with tandem mass spectrometry

Moldoveanu

Scott

Zhu³

2015

J of Separation Science

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Bioactive botanicals contain natural compounds with specific biological activity, such as antibacterial, antioxidant, immune stimulating, and taste improving. A full characterization of the chemical composition of these botanicals is frequently necessary. A study of small carbohydrates from the plant materials of 18 bioactive botanicals is further described. The study presents the identification of the carbohydrate using a gas chromatographic-mass spectrometric analysis that allows detection of molecules as large as maltotetraose, after changing them into trimethylsilyl derivatives. A number of carbohydrates in the plant (fructose, glucose, mannose, sucrose, maltose, xylose, sorbitol, and myo-, chiro-, and scyllo-inositols) were quantitated using a novel liquid chromatography with tandem mass spectrometric technique. Both techniques involved new method developments. The gas chromatography with mass spectrometric analysis involved derivatization and separation on a Rxi(®)-5Sil MS column with H2 as a carrier gas. The liquid chromatographic separation was obtained using a hydrophilic interaction type column, YMC-PAC Polyamine II. The tandem mass spectrometer used an electrospray ionization source in multiple reaction monitoring positive ion mode with the detection of the adducts of the carbohydrates with Cs(+) ions. The validated quantitative procedure showed excellent precision and accuracy allowing the analysis in a wide range of concentrations of the analytes.

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“…For carbohydrates, hydrophilic interaction chromatography (HILIC) and ion‐exchange chromatography (Dvořáčková and others ) are widely used. Although both hydrophilic interaction and ion exchange are effective in the separation, the former is more commonly used in the separation of mono‐ and oligosaccharides, and the latter for mono‐ and disaccharides.…”

Section: Hplc Analysismentioning

confidence: 99%

Chromatographic Methods for the Determination of Carbohydrates and Organic Acids in Foods of Animal Origin

Costa

Conté-Júnior

2015

Comp Rev Food Sci Food Safe

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Carbohydrates are ubiquitous and range from simple monosaccharides to large complex polysaccharides. Organic acids are compounds with acidic properties. Both occur naturally in many foods and in fermented products. Organic acids are usually derived from the hydrolysis of carbohydrates by microorganisms such as lactic acid bacteria. These bacteria convert carbohydrates into energy required for growth, since they are not equipped with the enzymes necessary for respiration and are unable to perform oxidative phosphorylation. Determination of carbohydrates and organic acids in foods of animal origin is important, since they contribute to flavor and texture. Their presence and proportions can affect the chemical and sensory characteristics of a food matrix and they can provide information on nutritional properties of food and the means to optimize selected technological processes. Furthermore, the levels of carbohydrate and organic acid are important to monitor bacterial growth and activity. Actually, these compounds can be quantified by several methods including high-performance liquid chromatography (HPLC) and gas chromatography (GC). High-performance liquid chromatography has been widely used to analyze carbohydrates and nonvolatile organic acids, while gas chromatography has been used to determine the volatile organic acids in complex matrices. This contribution provides an overview of chromatographic methods (HPLC and GC) used to analyze carbohydrates and organic acids in foods of animal origin.

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Carbohydrate analysis: From sample preparation to HPLC on different stationary phases coupled with evaporative light‐scattering detection

Cited by 51 publications

References 87 publications

Effect of cultivar, cultivation year and dehulling on raffinose family oligosaccharides in Australian sweet lupin (Lupinus angustifolius L.)

Effect of cultivar, cultivation year and dehulling on raffinose family oligosaccharides in Australian sweet lupin (Lupinus angustifolius L.)

Analysis of small carbohydrates in several bioactive botanicals by gas chromatography with mass spectrometry and liquid chromatography with tandem mass spectrometry

Chromatographic Methods for the Determination of Carbohydrates and Organic Acids in Foods of Animal Origin

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