Simultaneous determination of non-steroidal anti-inflammatory drugs in pharmaceutical formulations and human serum by reversed phase high performance liquid chromatography

Nawaz, Muhammad

doi:10.1590/s0100-40422012000500014

Cited by 5 publications

(2 citation statements)

References 16 publications

(16 reference statements)

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“…After two hours of administration, blood sample (5 mL) were withdrawn from the vein of each subject by using medical syringe and then transferred immediately into centrifuging tubes. The content of each tube was centrifuged for 15 min to separate the serum from the whole blood and all serum samples were kept freeze in refrigerator until analysis [40]. The serum samples were thawed at ambient temperature.…”

Section: Preparation Of Serum Samplesmentioning

confidence: 99%

An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals

Khammas¹

2014

SJAC

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A method was established for the determination of ciprofloxacin hydrochloride (CIPRO) in the blood serum and pharmaceutical samples by a combination of cloud point extraction with UV-Vis spectrophotometry. The method included the formation of Fe (III) -CIPRO complex in presence of dilute H 2 SO 4 , followed the extraction of the complex by cloud point extraction using non-ionic surfactant Triton X-114. The surfactant phase extracted was dissolved in little amount of solvent and the CIPRO quantity measured spectrophotometerically at absorption maximum of 437 nm. The effect of the experimental factors which affect the CPE efficiency was optimized by classical method. The preconcentration and enrichment factors were found to be 40 and 143 fold respectively, achieving the detection limit of 0.77 µg mL -1 and linear range of 2.5-120 µg mL -1 with correlation coefficient of 0.9984, under the optimum conditions. The average recovery percentage of CIPRO was of 98.89±0.87% and the precision in the range of 1.15-2.85%. The suggested method was applied for the determination of CIPRO in the serum samples of different subjects orally administrated for two hours with Emicipro 500, in addition to drug formulations like UFEXIL ® infusion and Ciprodar ® tablets. The experimental values were agreed with the quoted values that stated by the manufacturer' companies.

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Section: Preparation Of Serum Samplesmentioning

confidence: 99%

An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals

Khammas¹

2014

SJAC

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“…After two hours of administration, blood sample (5 mL) was withdrawn from the vein of each subject by using medical syringe and then transferred immediately into centrifuging tubes. The content of each tube was centrifuged for 15 min to remove the serum from the whole blood and all serum samples were kept freeze in refrigerator until analysis 24 . The serum samples were thawed at ambient temperature and 0.2 mL of each serum sample was transferred into a10 mL volumetric flask and followed the general CPE procedure for NOR.…”

Section: Preparation Of Serum Samples For Nor Detectionmentioning

confidence: 99%

Cloud Point Extraction Spectrophotometric Method for Mutual Determination of Norfloxacin and Iron(III) in Human Serum and Drug Formulations

Khammas¹,

Mubdir²

2015

Chem Sci Trans

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An eco-friendly procedure was developed for dual detection of drug norfloxacin (NOR) and iron(III) ions in biological and pharmaceutical samples via the coupling of cloud-point extraction procedure with molecular spectrophotometry. The drug norfloxacin reacts with Fe(III) ions in dilute acidic medium to form a colored hydrophobic (Fe(III)-NOR) complex which initially extracted into micelles of Triton x-114 as a mediated extractant followed the determination of both NOR and Fe(III) ions individually, by using spectrophotometry at same wavelength maximum of 432 nm. To achieve this goal, all experimental variables for both target analytes were previously optimized. The results have shown that the preconcentration factors of 83 and 71 for NOR and Fe(II) leading to achieve a limit of detection of 0.692 μg mL -1 3.42 ng mL -1 with linear range of 2.5-125 μgmL -1 (r= 0.9998) and 5-150 ng mL -1 (r=0.9997) respectively. The mean recovery percentage of 98.95±1.09% and 98.77±2.29%; the precision (RSD%) ranged between 0.04-0.66% and 0.59-0.97% were obtained for NOR and Fe(III) respectively. The proposed method was used for the determination of NOR in the serum samples of different subjects orally administration for two hours with Norfloxacin tablets BP 400 mg, in addition to drug formulations like Norflox Eye/Ear drops, While iron was determined in four selected drug formulations and in both cases, the experimental values are agreed with the quoted values that stated by the manufacturer company,

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Solid-Phase Fluorimetric Determination of Some Nonsteroidal Anti-Inflammatory Drugs in Medicines with the Aid of a Smartphone

2021

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Simultaneous determination of non-steroidal anti-inflammatory drugs in pharmaceutical formulations and human serum by reversed phase high performance liquid chromatography

Cited by 5 publications

References 16 publications

An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals

An Eco-Friendly Method for Extraction and Determination of Ciprofloxacin in Blood Serum and Pharmaceuticals

Cloud Point Extraction Spectrophotometric Method for Mutual Determination of Norfloxacin and Iron(III) in Human Serum and Drug Formulations

Solid-Phase Fluorimetric Determination of Some Nonsteroidal Anti-Inflammatory Drugs in Medicines with the Aid of a Smartphone

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