Optimization of a HPLC procedure for simultaneous determination of cisplatin and the complex cis,cis,trans-diamminedichlorodihydroxoplatinum(IV) in aqueous solutions

Abstract

Recebido em 10/9/10; aceito em 1/3/11; publicado na web em 5/5/11 A RP-HPLC procedure for the simultaneous determination of cisplatin and the complex cis,cis,trans-diamminedichlorodihydroxoplatinum(IV), was development. The developed procedure was validated in terms of linearity, accuracy, precision, limits of detection (LOD), limits of quantification (LOQ) and specificity. The limits of detection (LOD) , for cisplatin and cis,cis,trans-diamminedichlorodihydroxoplatinum(IV), respectively. The average recoveries of cisplatin and cis,cis,trans-diamminedichlorodihydroxoplatinum(IV) was 100.6% ± 1.4 and 101.2% ± 1.1, respectively. Intermediate (inter-day) precision, repeatability and specificity of the procedure for hydrolysis products of cisplatin were studied. The results of the study showed that the proposed RP-HPLC procedure is simple, rapid, precise, accurate and specific.

Aggregation Processes in Hybrid Nanosystem Polymer/Nanosilver/Cisplatin

“…It is known that the cis-Pt absorption spectrum in water has the absorption bands in the interval 200-250 and at 305 nm [36]. For the silver nanoparticles, the interval from 350 nm to 450 nm is typical [37], i.e.…”

Direct estimation of ethanol as a negative peak from alcoholic beverages and fermentation broths by reversed phase-HPLC

“…The minimum detectable range was calculated to be 3.3v/a and the minimum quantiable limit was calculated to be 10v/a where 'v' is the standard deviation and 'a' is the slope of the calibration plot as per ICH guidelines. 13,15,17,18 Test sample analysis Alcoholic beverages. The need for sample preparation may purely depend on the nature of the analyte.…”