Determinação direta de Ca, Mg, Mn e Zn em amostras de leite de búfala da Ilha de Marajó por espectrometria de absorção atômica com chama (FAAS)

Pereira, João B.; Fernandes, Kelly G.; Müller, Regina Céli Sarkis; Nóbrega, Joaquim A.; Palheta, Dulcidéia C.

doi:10.1590/s0100-40422009000900018

Cited by 6 publications

(4 citation statements)

References 4 publications

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“…Several industries use buffalo milk in cheese production due to the superiority of its component raw materials compared to cow milk (Bittencourt et al, 2013). However, consumption of food products from this production chain is informal due to lack of regulation of standards for the identity and quality of buffalo milk and its derivatives, which complicates the control and inspection measures (Pereira Júnior et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Detection of fraud by addition cow s milk in cheese buffalo and its connection with seasonality

Cardoso¹,

Oliveira²,

Silva³

et al. 2019

Revista Brasileira De Ciência Veterinária

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The seasons influence the production of buffalos' milk. Because of this, the producers may produce a mixture of buffalo and bovine milk during cheese production in periods of low production. Therefore, the present work aimed to investigate fraud in buffalo cheese and the relationship between seasonality and different physicochemical properties of buffalo cheeses produced and marketed in eastern Amazonia. We obtained commercial samples of buffalo cheese during two Amazonian climatic periods from commercial points of Marajó-Pará, Brazil. After collection, there were lipid, protein, ash, and humidity analyses. Determination of carbohydrates and energy values was also performed for the nutritional characterization of samples, as well as for mPCR analysis to detect buffalo and/or bovine DNA. DNA extraction protocol of the samples was standardized and two pairs were used for the mPCR reaction, amplifying fragments of approximately 220 bp for Bubalus bubalis DNA and 346 bp fragments for Bos taurus DNA. Among the samples acquired in the rainy season, we observed that 33% were inadequately labeled, indicating fraud from cow's milk incorporation and fraud from substitution of raw material. From the nine samples obtained in the dry season, all the samples showed cow's milk incorporation fraud. The highest fraud rate coincided with the period of low milk production from buffalo and there was a difference in composition between fraudulent and non-fraudulent cheeses. Therefore, seasonality influences increase in cattle milk for the production of buffalo cheese, and this adulteration may decrease the nutritional content of the product.

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Section: Discussionmentioning

confidence: 99%

Detection of fraud by addition cow s milk in cheese buffalo and its connection with seasonality

Cardoso¹,

Oliveira²,

Silva³

et al. 2019

Revista Brasileira De Ciência Veterinária

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“…The slope and correlation coefficient (r) of the FAAS calibration curve prepared in the presence of 1.0% (v/v) of HNO3 were 0.4688 and 0.9994, respectively. Moreover, limits of detection (LOD) calculated as three times the standard deviation of the blank (n=10)/slope was 0.02 mg L -1 and quantification (LOQ) (3 × LOD) was 0.03 mg L -1 for Zn, which are appropriate for Zn determination in the evaluated milk samples 26 .…”

Section: Total Zn and Species Determinationmentioning

confidence: 98%

Zinc fractionation in cow, goat, sheep and soybean milk samples using gel-electrophoresis and determination by electrothermal atomic absorption spectrometry (ETAAS)

et al. 2021

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A screening method for zinc levels in different milk samples (raw cow, raw sheep, UHT cow, UHT goat and soybean milk base) was performed to establish the Zn levels’ differences in protein samples. The samples were digested in a cavity microwave oven and the total Zn levels in the extracts were determined by flame absorption atomic spectrometry (FAAS). The protein separation was performed by urea polyacrylamide gel electrophoresis (UREA-PAGE). Protein bands were digested in the cavity microwave oven and Zn-protein analysis was further conducted by electrothermal atomic absorption spectrometry (ETAAS). The results showed that Zn is mainly bound to 32 kDa (β-casein) protein (17.0 ± 2.0%) in UHT cow and 24 kDa (α-casein) protein (9.0 ± 0.6%) in raw sheep milk. This method provided quantitative information regarding Zn species present in the protein fractions of the milk samples. The accuracy was evaluated using certified reference material (whole milk powder, NIST 8435) with statistically equivalent concentrations (Student’s t-test) for total Zn and by addition and recovery experiments applied to measure Zn-protein. The recovered values were in the 92-110% range.

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“…Além de ser um método bastante eficaz para identificação de elementos traços em gêneros alimentícios, investigações por contaminação ambiental e para outras análises que possam ser identificadas pelo teste em questão. Encontramos o método de investigação de metais por absorção atômica em leite de animais, como, por exemplo, em leite de búfala 7 . Com base nos resultados, nota-se a necessidade de inserção do método para que faça parte do controle de qualidade desses leites em Bancos de Leite, pois se trata da nutrição de recém-nascidos, com carência de um metal específico para seu desenvolvimento.…”

Section: Introductionunclassified

Análise de elemento traço por absorção atômica em amostras de leite humano coletadas no Banco de Leite do Amapá

Monteiro

Messias

2014

Mundo Saúde

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Este trabalho avaliou a concentração de minerais nos leites que são doados ao Banco de Leite do Estado do Amapá, por meio da técnica de absorção atômica, com o objetivo de fomentar a importância dessa análise na rotina dos Bancos de Leite para auxiliar nos tratamentos terapêuticos de recém-nascidos que possuem carência por determinado mineral. Após a quantificação desses teores, o leite poderia ter um fim intencional para tratamentos terapêuticos de recém-nascidos que possuem carência por determinado mineral. As análises quantitativas dos elementos presentes no leite foram para os teores de Ca, Cu, Zn, Mg, Mn, Na, Cd, Fe, Pb e K. Esses elementos foram escolhidos devido à importância e ao valor nutricional para absorção por recém-nascidos, assim como pela possibilidade de causar intoxicação aguda, como é o caso do Cd e o Pb, quais são comuns, em níveis baixos, em determinados alimentos e bebidas. Por exemplo, em uma amostra de leite foi verificado um teor de 1 ppm de Cd, sendo portanto um valor na média de contaminação. Os resultados das análises foram divididos em 4 zonas, representando os pontos de coleta do Estado do Amapá. Em duas zonas foram observados altos índices de Mn e Na. Ao se verificar todos os pontos do trabalho, notou-se que os testes foram satisfatórios, principalmente quanto ao comparativo com outros testes de absorção atômica e rótulos de leites comerciais. Enfim, observou-se nesse estudo que o método investigativo de metais em leite humano é de suma importância para fazer parte da rotina de Banco de Leites, principalmente para fins terapêuticos e nutricionais, analisando a qualidade e a quantidade de elementos químicos constituintes desse leite.

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Determinação direta de Ca, Mg, Mn e Zn em amostras de leite de búfala da Ilha de Marajó por espectrometria de absorção atômica com chama (FAAS)

Cited by 6 publications

References 4 publications

Detection of fraud by addition cow s milk in cheese buffalo and its connection with seasonality

Detection of fraud by addition cow s milk in cheese buffalo and its connection with seasonality

Zinc fractionation in cow, goat, sheep and soybean milk samples using gel-electrophoresis and determination by electrothermal atomic absorption spectrometry (ETAAS)

Análise de elemento traço por absorção atômica em amostras de leite humano coletadas no Banco de Leite do Amapá

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