In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)

Soares, Taliane Leila; Jesus, O. N. de; Santos-Serejo, Janay Almeida dos; Oliveira, E. J. de

doi:10.1590/s0100-29452013000400023

Cited by 38 publications

(26 citation statements)

References 22 publications

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“…Pollen viability was detected regardless of the dye used, however, the only percentages of viable pollens higher than 80% were in the treatments with CA and AS, and these dyes could have overestimated the values of viable pollens as reported by other authors in different species [28,29]. Colorimetric techniques overestimate pollen viability, while the in vitro test underestimates it [30].…”

Section: Resultsmentioning

confidence: 99%

Pollen Viability in Eggplant Using Colorimetric and In vitro Techniques

Valadares

Lima

Nóbrega

et al. 2019

JEAI

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The causes of fruit abortion, in eggplants, may be related to the absence of viable pollen under high temperatures, common in the Northeast region of Brazil, resulting in a reduction in the number of fruits per plant and consequently in productivity. The objective of this study was to evaluate the techniques of identification of PV – pollen viability in eggplant, as well as to correlate these results with the FFI – Fruit Fixation Index, NFP – Number of Fruits per Plant and PP – Production per Plant obtained in cultivation under high temperatures. The experiment was conducted at the Federal Rural University of Pernambuco, Recife, Brazil, between September and December 2017. The experiment design was a randomized block design with four replications, in the 7 x 4 factorial scheme (7 genotypes x 4 evaluation of PV) containing four plants per experimental plot. The results showed that the different techniques used detected viable pollens in the genotypes evaluated in smaller and in larger proportions. The highest percentages were observed in the treatments with CA – Carmine Acetic and AS – Alexander Solution and these overestimated the PV (>90%). In the test with TTC – 2,3,5-triphenyltetrazolium chloride (25%), the viability estimation was equivalent to the in vitro germination test, however the genetic correlations were null and/or very low between these techniques and the FFI, NFP and PP traits. The genetic correlations between the results obtained with CA and AS with FFI, NFP and PP under conditions of high temperatures were high and positive and suggest that the selection based on PV only, except with the use of TTC solution, may be efficient for indirect selection of genotypes with high FFI, NFP and PP.

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Section: Resultsmentioning

confidence: 99%

Pollen Viability in Eggplant Using Colorimetric and In vitro Techniques

Valadares

Lima

Nóbrega

et al. 2019

JEAI

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“…However, despite these drawbacks, in vitro germination simulates the closest condition to in planta pollen germination (Soares et al 2013) and has been considered a very useful methodology for this purpose.…”

Section: In Vitro Pollen Germinationmentioning

confidence: 99%

Pollen morphology and viability in Bromeliaceae

Souza

Rossi

et al. 2017

An. Acad. Bras. Ciênc.

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Pollen morphology characterization is important in taxonomy, conservation and plant breeding, and pollen viability studies can support breeding programs. This study investigated pollen morphology and male fertility in 18 species of Bromeliaceae with ornamental potential. For morphological characterization, pollen grains were acetolyzed and characterization of exine was done using scanning and transmission electron microscopy. Pollen viability was investigated by in vitro germination and histochemical tests. Species belonging to Aechmea and Ananas genera presented medium size pollen, except for Ae. fasciata, with large pollen. Al. nahoumii, P. sagenarius and the Vriesea species analyzed showed large pollen, except for V. carinata, with very large pollen. Pollen of Aechmea, Ananas and P. sagenarius presented bilateral symmetry, diporate, exine varying from tectate to semitectate. Al. nahoumii and Vriesea species presented pollen with bilateral symmetry, monocolpate; exine was semitectate, reticulate and heterobrochate. Germination percentage and tube growth were greater in SM and BKM media. Histochemical tests showed pollen viability above 70% for all species, except for Ananas sp. (40%). Pollen morphology is important for the identification of species, especially in this family, which contains a large number of species. High rates of viability favor fertilization and seed production, essential for efficient hybrid production and conservation.

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“…A culture medium containing 0.03% Ca(NO 3 )4H 2 O, 0.02% Mg(SO 4 )7H 2 O, 0.01% KNO 3 , 0.01% H 3 BO 3 , 15% sucrose and 0.8% agar, with pH of 7.0 for Passiflora spp. gave higher percentage of pollen grains germinated [21]; anthesis was founded to be the best time to collect pollen, promoting high viability and germination [21]. The study [14] of in vitro pollen germination in Ricinus communis L. found that pollen collected at anthesis into a 12% sucrose solution supplemented with 100 ppm boric acid resulted in an 80% germination percentage and 195 µm long pollen tubes.…”

Section: Viability Test Of Pollen Collected At Anthesismentioning

confidence: 99%

Pollen Viability of Aeschynanthus tricolor Hook.

Gandadikusumah

Wawangningrum

Rahayu

2017

JTLS

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Aeschynanthus (Gesneriaceae) is climbing or trailing epiphytes from the tropical forests of South-East Asia. Several species are widely cultivated for their attractive flowers, which usually scarlet with long corolla tubes and probably bird pollinated.This study was carried out on Aeschynanthus tricolor Hook. to determine in vitro germinability of (1) pollen collected from flowers at anthesis, on the day before anthesis, or up to five days after anthesis and (2) pollen collected on the day of anthesis and then stored at -20 o C for 127 days. Germinability tests were carried out on pollen grains after stored for four hours in 10% sucrose solution. The highest in vitro germination percentage observed was pollen collected on the day of anthesis (96.3%) and the lowest was pollen collected on the fifth day after anthesis (5.6%). The germination percentage of pollen after stored at -20 o C was 98% at beginning and 22% after 127 days. From this research we can conclude that pollen of A. tricolor can be successfully collected and then stored for some time, will be practical benefit for plant breeding and conservation purposes.

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In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)

Cited by 38 publications

References 22 publications

Pollen Viability in Eggplant Using Colorimetric and In vitro Techniques

Pollen Viability in Eggplant Using Colorimetric and In vitro Techniques

Pollen morphology and viability in Bromeliaceae

Pollen Viability of Aeschynanthus tricolor Hook.

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