Potencial de multiplicação in vitro de cultivares de framboeseira

Abstract

RESUMO -Objetivou-se avaliar o potencial de multiplicação in vitro de quatro das principais cultivares de framboeseira utilizadas no Brasil: Autumn Bliss, Batum, Dorman Red e Heritage. Utilizou-se protocolo empregado em laboratórios comerciais. A desinfestação dos explantes foi realizada em soluções à base de álcool e hipoclorito de sódio; a cultura dos meristemas em meio semissólido MS com 1 mg L IN VITRO MULTIPLICATION RATE OF RASPBERRY CULTIVARSABSTRACT -The aim of this work was to evaluate the in vitro potential of multiplication of the four main raspberry cultivars used in Brazil: Autumn Bliss, Batum, Dorman Red and Heritage. A commercial micropropagation protocol was used. The explant desinfestation was carried out in solution of alcohol and sodium hypochlorite. The meristem culture was carried out in semi-solid MS medium with 1 mg L -1 BAP, 0.01 mg L -1 ANA and 0.1 mg L -1 AG 3 ; the explant multiplication in MS medium with 0.8 mg L -1 BAP and 15 mg L -1 iron sulphate; and the rooting in ½MS medium with 0.1 mg L -1 ANA, always at 25±4 o C, 20 μE m -2 s -1 and 16 hours photoperiod. Sixty meristems of each cultivar were used, evaluating the multiplication rate and the level of contamination, vitrification and oxidation during the phases of in vitro establishment (60 days), multiplication (six subcultures of 40 days) and rooting (30 days). The raspberry cultivars studied showed high genetic variability concerning in vitro multiplication rate. The estimated number of plants obtained per meristem for the described micropropagation system was of 56664 for 'Autumn Bliss', 6692 for 'Heritage', 1942 for 'Batum' and 696 for 'Dorman Red'. The quantification of this in vitro variability response is important for planning the plant production in commercial laboratories.

Micropropagation protocol for the wild Brazilian greenberry (Rubus erythroclados)

“…Tupy, which can be multiplied in vitro without the addition of IBA in the culture medium, as observed in this study. A commercial micropropagation protocol using 3.56 μM BA obtained an average multiplication rate of 6.2 shoots per explant with seven blackberries cultivars in six subcultures (Oliveira et al, 2008), and, in a similar protocol with the same BA concentration for four raspberry cultivars, the average multiplication rate was 4.6 (Oliveira and Nino, 2009). The concentration of 5 μM BA also provided the greatest shoot growth in both subcultures (Tab.…”

Micropropagação de framboeseira em diferentes concentrações de ferro

“…Trata-se de uma cultura de baixo custo de implantação, que demanda bastante mão-de-obra, mas que apresenta alta rentabilidade por hectare (OLIVEIRA & NINO, 2009). No Brasil, a produção de framboesa é realizada principalmente nos Estados de São Paulo, Minas Gerais e Rio Grande do Sul, com uma área cultivada de 60ha e produção anual de 180 toneladas, sendo as cultivares 'Heritage' e 'Batum' as mais plantadas (IBGE, 2010).…”