Variabilidade genética de acessos obtidos de populações cultivadas e silvestres de maracujazeiro-doce com base em marcadores rapd

Abstract

RESUMO-O maracujazeiro-doce (Passiflora alata Curtis), devido a preços diferenciados, vem ganhando importância dentro do mercado de frutas in natura. O melhoramento genético é fundamental para elevar a qualidade e a produtividade da cultura. Os marcadores moleculares do DNA têm sido muito úteis por permitirem a obtenção de um número praticamente ilimitado de polimorfismo genético sem influência do ambiente. Objetivou-se, neste trabalho, estudar a variabilidade genética de 17 acessos de maracujá-doce, com base em marcadores moleculares RAPD. Um acesso de P. quadrangularis e um de P. edulis foram utilizados como outgroups. Amostras de DNA genômico de cada acesso foram extraídas e 11 iniciadores decâmeros (OPD 04; 07; 08 e16; OPE 18 e 20; OPF 01 e 14; OPG 08; OPH 12 e 16) foram utilizados para a obtenção dos marcadores. Os marcadores obtidos foram convertidos em uma matriz de dados binários, a partir da qual foram estimadas as distâncias genéticas entre os acessos e realizadas análises de agrupamento e de dispersão gráfica. Do total de marcadores, considerando-se apenas os acessos de P. alata, observaramse 87 (62,12%) bandas polimórficas, evidenciando a grande variabilidade intraespecífica. A análise de agrupamento realizada com base nas distâncias genéticas permitiu subdividir os 17 acessos de P. alata em, pelo menos, cinco grupos de similaridade genética. Os acessos silvestres foram os que mais contribuíram para a ampliação da base genética dos materiais estudados, abrindo perspectivas para o uso desses materiais em programas de melhoramento. Termos para indexação: Passiflora alata Curtis., biotecnologia, melhoramento genético. GENETIC DIVERSITY OBTAINED FROM CULTIVATED POPULATION AND NATIVE ACCESSES OF SEEWT PASSION FRUIT BASED ON RAPD MARKERSABSTRACT -Sweet passion fruit (Passiflora alata Curtis) is gaining importance in the in natura fruit market due to differential value. Genetic breeding is crucial to improve crop quality and productivity. Molecular markers of DNA have been very useful by allowing obtaining a virtually unlimited number of genetic polymorphism without environment influence. This work's objective was to study the genetic variability of 17 sweet passion fruit accesses, using RAPD molecular markers. One access of P. quadrangularis and another of P. edulis were used as outgroups. Genomic DNA samples of each one of them were extracted and 11 decamers primers (OPD 04, 07, 08 e16; OPE 18 and 20; OPF 01 and 14; OPG 08; OPH 12 and 16) were used to obtain the markers. The markers have been converted into a matrix of binary data, used as base to estimate genetic distances between accesses and to perform grouping and graphic dispersion analysis. From the total amount of markers, considering only P. alata accesses, it was observed 87 (62.12%) polymorphic bands, showing great intraspecific variability. Grouping analysis based on genetic distances allowed to subdivide 17 P. alata accesses in, at least, five groups of genetic similarity. The wild accesses contributed the most to the genetic basis expansion ...

Short Communication Genetic variation in a wild population of the ‘sleep’ passion fruit (Passiflora setacea) based on molecular markers

“…A total of 84 polymorphic (88%) and 11 monomorphic (12%) markers were generated (an average of 7.9) ( Table 2). These results exhibit a polymorphism higher than reported by Bellon et al (2005), who worked with wild accessions of P. alata Curtis (62.1% of polymorphic bands). The high average bands per primer are also evidence of high variability.…”

“…Bellon et al (2005), who working with P. alata observed high variability (an average number of 12.05 bands per primer) and Cerqueira-Silva et al (2010b), who working with P. tritae observed an average number of 7.46 bands per primer. With the commercial species P. edulis, Bellon et al (2007) observed an average number of 14.4 bands per primer, while Cerqueira-Silva et al (2010a) studying the same species observed a lower average number of bands per primer (5.7).…”

“…In molecular diversity characterizations, RAPD markers (random amplified poly-morphic DNA) (Williams et al, 1990) have been widely used in Passiflora species, such as P. alata (Bellon et al, 2005), P. edulis Sims (Bellon et al, 2007;Cerqueira-Silva et al, 2010a), P. trintae (Cerqueira-Silva et al, 2010b), and Passiflora spp (Viana et al, 2010). We quantified, by RAPD markers and complement of the Dice index, the dissimilarity of wild genotypes of 'sleep' passion fruit, originating from the region of Vitória da Conquista, Bahia, Brazil, and belonging to the CAGT-Passiflora/UESB 'Planalto de Conquista'.…”

Genetic diversity in yellow passion fruit (Passiflora edulis Sims) based on RAPD

“…The P. edulis genotypes used in this study were originated from openpollinated genotypes of field production, and despite indirectly, they were the result of participatory plant breeding or local selections performed by the producers themselves. On the other hand, Bellon et al (2007) and Bellon et al (2009) assessed plants from wild and commercial accessions together, contributing to a greater variability between members of the population.…”

Phenotyping of Passiflora edulis, P. setacea, and their hybrids by a multivariate approach

“…Among the wild species that were indicated as potential sources of resistance to diseases affecting passion fruit, Passiflora setacea DC deserves special attention because it is resistant to CABMV (Junqueira et al, 2006;Bellon et al, 2009). The characterization of a segregating population by means of morphoagronomic descriptors is particularly important for breeders because of the wide variability that is available in the population that remains little known or exploited.…”