Tipo de luz na multiplicação in vitro de framboeseira (Rubus idaeus L.) 'Batum'

Erig, Alan Cristiano; Schuch, Márcia Wulff

doi:10.1590/s0100-29452005000300035

Cited by 15 publications

(7 citation statements)

References 6 publications

(6 reference statements)

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“…Para framboesa (Rubus idaeus L.), maior número de brotos foi obtido com as luzes verde (3,55) e vermelha (3,14) (ERIG & SCHUCH, 2005). Plantas cultivadas sob luz azul podem resultar em altas taxas de brotações laterais, em razão da quebra da dominância apical causada pela degradação de auxinas nessa faixa do espectro (CHEE & POOL, 1989;SILVA & DEBERGH, 1997).…”

Section: Folhas (Nf)unclassified

Multiplicação in vitro de copo-de-leite: espectros de luz e sacarose

et al. 2009

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O espectro luminoso pode alterar a morfogênese das plantas por meio de uma série de processos mediados por receptores de luz, principalmente na região do vermelho e azul. O objetivo deste trabalho foi determinar um possível tipo de luz mais ativo que a luz branca, de forma a aumentar a eficiência da multiplicação in vitro de copo-de-leite e obter um nível ótimo de sacarose. Para isso, os tratamentos consistiram em quatro diferentes tipos de luz, sob os quais os explantes cresceram (branca, vermelha, azul e verde), fornecidos pelo espectro luminoso das lâmpadas fluorescentes coloridas T8 20W Ecolume 05/1 e sacarose (0; 15; 30; 45 e 60g L-1). O meio de cultura constituiu-se dos sais e das vitaminas de MS, adicionado de 5mg L-1 de BAP e 5,5g L-1 de ágar. O tipo de luz não influencia a multiplicação in vitro de copo-de-leite, e o maior número de brotos (2,0) é obtido em 45g L-1 de sacarose. Observou-se maior número de folhas em luz branca (3,8) e azul (2,9), com 15 e 60g L-1 de sacarose, respectivamente. Maior comprimento dos brotos (3,7cm) foi observado em 60g L-1 de sacarose, e maior fitomassa fresca da parte aérea (1,07g) foi observada com a utilização de 60g L-1 de sacarose.

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Section: Folhas (Nf)unclassified

Multiplicação in vitro de copo-de-leite: espectros de luz e sacarose

et al. 2009

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“…For acclimatization, generally, plantlets rooted in vitro on media favorable for rooting are used (Bobrowski et al, 1996;Erig and Schuch, 2005;Najaf-Abadi and Hamidoghli, 2009;Radmann et al, 2003;Ružic andLaziž, 2006, 2007). Hazarika (2003), in a review on ex vitro acclimatization, mentions that during transition, better plantlet development, stomata functioning, and synthesis of epicuticular wax can be achieved by lowering air humidity in the culture vessels, by the use of growth retardants like paclobutrazol or Cycocel, and the use of antitranspirants.…”

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An Efficient Ex Vitro Rooting and Acclimatization Method for Horticultural Plants Using Float Hydroculture

Clapa

Fira²,

Joshee³

2013

horts

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This article presents a new acclimatization method, ex vitro acclimatization in float hydroculture. This protocol ensures the ex vitro acclimatization of the plantlets obtained in vitro in the rooting stage or the ex vitro rooting and acclimatization of the shoots obtained in the multiplication stage. Our hydroculture method is different from the techniques of flotation hydroculture because no fertilizers or plant growth regulators or other biostimulators are used and water oxygenation by bubbling is not provided. Ex vitro rooting and acclimatization in one stage without an in vitro rooting stage in floating cell trays was successfully carried out in Rubus fruticosus and Rosa hybrida cultivars, whereas ex vitro acclimatization of in vitro-rooted plantlets was successfully carried out in Rubus idaeus, Ribes nigrum, Prunus cerasus × P. canescens, Lycium barbarum (Goji berry), Amelanchier canadensis, Drosera rotundifolia, Drosera capillaris, and Nephrolepis sp. In another set of experiments, the floating cell trays were replaced with layers of floating perlite and the latter method was also tested for non-rooted shoots obtained in vitro in the multiplication stage or rooted plantlets obtained in the rooting stage. Direct ex vitro rooting in floating perlite was successful in ‘Tayberry’ (Rubus fruticosus × Rubus idaeus) (78.12% rooting); Amelanchier canadensis, Rubus fruticosus ‘Chester’, Rubus idaeus ‘Erntesegen’, Vaccinium macrocarpon, and Vaccinium corymbosum with rooting percentages above 80%; and Rubus idaeus ‘Willamette’ and Rosa hybrida ‘Cristiana’ with rooting percentages above 80%.

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“…A 'Heritage' é uma das cultivares mais plantadas no mundo, sendo citada como bastante vigorosa a campo (Raseira et al, 2004). Quanto à 'Batum', Erig & Schuch (2005) obtiveram uma taxa média de multiplicação (5,3) superior à do presente trabalho (4,1), porém utilizaram um subcultivo mais longo, de 45 dias, e realizaram a quantificação apenas por um subcultivo. A cv.…”

Section: In Vitro Multiplication Rate Of Raspberry Cultivarsunclassified

Potencial de multiplicação in vitro de cultivares de framboeseira

Oliveira

Nino

2009

Rev. Bras. Frutic.

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RESUMO -Objetivou-se avaliar o potencial de multiplicação in vitro de quatro das principais cultivares de framboeseira utilizadas no Brasil: Autumn Bliss, Batum, Dorman Red e Heritage. Utilizou-se protocolo empregado em laboratórios comerciais. A desinfestação dos explantes foi realizada em soluções à base de álcool e hipoclorito de sódio; a cultura dos meristemas em meio semissólido MS com 1 mg L IN VITRO MULTIPLICATION RATE OF RASPBERRY CULTIVARSABSTRACT -The aim of this work was to evaluate the in vitro potential of multiplication of the four main raspberry cultivars used in Brazil: Autumn Bliss, Batum, Dorman Red and Heritage. A commercial micropropagation protocol was used. The explant desinfestation was carried out in solution of alcohol and sodium hypochlorite. The meristem culture was carried out in semi-solid MS medium with 1 mg L -1 BAP, 0.01 mg L -1 ANA and 0.1 mg L -1 AG 3 ; the explant multiplication in MS medium with 0.8 mg L -1 BAP and 15 mg L -1 iron sulphate; and the rooting in ½MS medium with 0.1 mg L -1 ANA, always at 25±4 o C, 20 μE m -2 s -1 and 16 hours photoperiod. Sixty meristems of each cultivar were used, evaluating the multiplication rate and the level of contamination, vitrification and oxidation during the phases of in vitro establishment (60 days), multiplication (six subcultures of 40 days) and rooting (30 days). The raspberry cultivars studied showed high genetic variability concerning in vitro multiplication rate. The estimated number of plants obtained per meristem for the described micropropagation system was of 56664 for 'Autumn Bliss', 6692 for 'Heritage', 1942 for 'Batum' and 696 for 'Dorman Red'. The quantification of this in vitro variability response is important for planning the plant production in commercial laboratories.

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Tipo de luz na multiplicação in vitro de framboeseira (Rubus idaeus L.) 'Batum'

Cited by 15 publications

References 6 publications

Multiplicação in vitro de copo-de-leite: espectros de luz e sacarose

Multiplicação in vitro de copo-de-leite: espectros de luz e sacarose

An Efficient Ex Vitro Rooting and Acclimatization Method for Horticultural Plants Using Float Hydroculture

Potencial de multiplicação in vitro de cultivares de framboeseira

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