An Efficient Ex Vitro Rooting and Acclimatization Method for Horticultural Plants Using Float Hydroculture

Abstract

<jats:p>This article presents a new acclimatization method, ex vitro acclimatization in float hydroculture. This protocol ensures the ex vitro acclimatization of the plantlets obtained in vitro in the rooting stage or the ex vitro rooting and acclimatization of the shoots obtained in the multiplication stage. Our hydroculture method is different from the techniques of flotation hydroculture because no fertilizers or plant growth regulators or other biostimulators are used and water oxygenation by bubbling is not provided. Ex vitro rooting and acclimatization in one stage without an in vitro rooting stage in floating cell trays was successfully carried out in <jats:italic>Rubus fruticosus</jats:italic> and <jats:italic>Rosa hybrida</jats:italic> cultivars, whereas ex vitro acclimatization of in vitro-rooted plantlets was successfully carried out in <jats:italic>Rubus idaeus, Ribes nigrum, Prunus cerasus</jats:italic> × <jats:italic>P. canescens, Lycium barbarum</jats:italic> (<jats:italic>Goji berry</jats:italic>), <jats:italic>Amelanchier canadensis, Drosera rotundifolia</jats:italic>, <jats:italic>Drosera capillaris</jats:italic>, and <jats:italic>Nephrolepis</jats:italic> sp. In another set of experiments, the floating cell trays were replaced with layers of floating perlite and the latter method was also tested for non-rooted shoots obtained in vitro in the multiplication stage or rooted plantlets obtained in the rooting stage. Direct ex vitro rooting in floating perlite was successful in ‘Tayberry’ (<jats:italic>Rubus fruticosus</jats:italic> × <jats:italic>Rubus idaeus</jats:italic>) (78.12% rooting); <jats:italic>Amelanchier canadensis</jats:italic>, <jats:italic>Rubus fruticosus</jats:italic> ‘Chester’, <jats:italic>Rubus idaeus</jats:italic> ‘Erntesegen’, <jats:italic>Vaccinium macrocarpon,</jats:italic> and <jats:italic>Vaccinium corymbosum</jats:italic> with rooting percentages above 80%; and <jats:italic>Rubus idaeus</jats:italic> ‘Willamette’ and <jats:italic>Rosa hybrida</jats:italic> ‘Cristiana’ with rooting percentages above 80%.</jats:p>

Comparison of antioxidant activity of the fruits derived from in vitro propagated and traditionally cultivated tayberry plants

“…Gonzalez et al proposed a mixture of peat and perlite (1:1, v/v) for hardening of blackberry, blueberry and raspberry, and 100% of rooted plants were adapted . Direct ex vitro rooting in floating perlite was successfully applied in tayberry plants, with78.12% rooting . In our study, the micropropagated tayberry plants and adapted under field conditions did not exhibit any morphological variations when compared with mother plants, and gave a large number of fruits.…”

Optimization of Micropropagation Protocol for Goji Berry (Lycium barbarum L.)

“…For the ex vitro acclimatization of plantlets rooted in vitro we recommend the use of floatation hydroculture due to its simplicity and reliability and due to the very high survival percentage obtained. This technique was developed at the Fruit Research Station Cluj (Clapa et al, 2013) and is effective for several species. It was inspired from float hydroponics, a very simple and effective hydroponic technique (Reed, 2009;Ross and Teffeau, 1995;Tyson, 1999).…”

In Vitro Propagation Of ‘Gisela 5’ Rootstock As Affected By Mineral Composition Of Media And Plant Growth Regulators

“…The 'Gisela 5' sweet cherry rootstock is propagated with greenwood, soft or hardwood cuttings (Exadaktylou et al 2009), but also micropropagation has been reported (Ružić et al 2000;Nacheva & Gercheva 2009;Bošnjak et al 2012;Clapa et al 2013;Šiško 2011. An efficiency in tissue culture propagation is strongly influenced by a mineral composition of culture medium and its interaction with other medium compounds, as water quality, sugars, growth regulators, vitamins, etc.…”