Marcadores Rapd Para Mapeamento Genético E Seleção De Híbridos De Citros

Oliveira, R. P. de; Cristofani, M; Machado, Marcos Antônio

doi:10.1590/s0100-29452001000300005

Cited by 5 publications

(3 citation statements)

References 13 publications

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“…These values are inferior to those found by Menéndez et al (1997), who in analyzing a cross between 2 genotypes of V. unguiculata (IT84S-2049 x 524) developed in Nigeria and California and contrasting for several morphological traits and resistance to pests and diseases found an average of approximately 55 fragments/primer and 12% polymorphism per combination of AFLP primer using a fluorescent system and semi-automatic sequencer. Oliveira et al (2001) obtained an average of 7.6 amplified bands using random amplification of polymorphic DNA markers in the genetic mapping and the selection of hybrids between Citrus reticulata Blanco and Citrus sinensis (L.) Osbeck. Boiteux et al (2000) have reported an average of 2.4 amplicons of random amplification of polymorphic DNA in the mapping of a nematode in an F 2 population of carrot.…”

Section: Discussionmentioning

confidence: 99%

Mapping of AFLP loci linked to tolerance to cowpea golden mosaic virus

Rodrigues¹,

Santos²,

Santana³

2012

Genet. Mol. Res.

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ABSTRACT. AFLP markers combined with the bulk segregant analysis methodology was used for the identification of molecular markers associated with the cowpea golden mosaic virus (CGMV) resistance gene in 286 F 2 cowpea plants derived from the cross IT97K-499-35 x Canapu T16. Segregation data in the F 2 population demonstrated that tolerance to CGMV is controlled by a single dominant gene. Among the 196 combinations of AFLP primers tested, which generated approximately 3800 amplicons, three markers linked to the CGMV resistance gene were identified: E.AAC/M.CCC 515 at 4.3 cM, E.AGG/M.CTT 280 at 14.2 cM and E.AAA/M.CAG 352 at 16.8 cM, with 50.4, 24.4, and 28.7 LOD scores, respectively; the former two markers flank the CGMV loci. These markers could be used for the development of 'sequence characterized amplified region' type markers or for greater saturation of this region, to increase the precision of assisted selection for the development of cowpea strains tolerant to CGMV.

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Section: Discussionmentioning

confidence: 99%

Mapping of AFLP loci linked to tolerance to cowpea golden mosaic virus

Rodrigues¹,

Santos²,

Santana³

2012

Genet. Mol. Res.

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“…RAPD has been used for different numbers of species, independent of the strategy of reproduction with success, like in Citrus (allogamous, Oliveira et al 2000, Oliveira et al 2001, Passiflora (allogamous, Crochemore et al 2003), Coffea (autogamous, Diniz et al 2005;allogamous, Ferrão et al 2008), Aspidosperma (allogamous, Torezan et al 2005) and Zea mays (self-fertilized, Souza et al 2008). The aim of this work was to study the genetic similarity among germplasm accessions of twelve clones of Macadamia from two selection groups, Hawaiian and Brazilian, to check the proximity of them with the germplasm analysed, through the use of RAPD markers.…”

Section: Introductionmentioning

confidence: 99%

Variability in Macadamia integrifolia by RAPD markers

Neto

Moryia

2010

Crop Breed. Appl. Biotechnol.

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-Macadamia is one of the most valuable commercial nuts and its culture is a good alternative for small farmers or for large properties with a low capacity for the mechanization of its areas. Macadamia clones, due to their morphological proximity exhibited some difficulties in field identification that could difficult the right plant distribution. The objective of this work was to genetically characterize, by RAPD markers, different macadamia clones under cultivation. Leaves from 'HAES-741', 'HAES-660', 'IAC-920BX', 'IAC-920', 'HAES-344', 'IAC-420', 'HAES-816', 'IAC-1.21', 'Shimada', 'IAC -Campinas B', 'IAC -Campinas A' and 'HAES-Aloha' were donated by Queen Nut Macadâmia. DNA was extracted and amplified with 15 primers and the binary data (presence/absence of bands) collected was used and dendrograms constructed. Clone identification, via RAPD was possible using primers Op G18, C19 and C7. Most of the genetic variation is inside the Brazilian or Hawaiian groups rather than between the groups and could allow inbreeding or croxssing between distant clones.

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“…Desde a década de 80, diversos tipos de marcadores moleculares baseados em DNA foram desenvolvidos, com variações no tipo de tecnologia utilizada para detectar o polimorfismo, visando a redução de custos, a facilidade de uso, reprodutibilidade e a obtenção de dados em larga escala (Schlötterer 2004). Os marcadores RFLP (Restriction Fragment Length Polymorphism (Botstein et al 1980), RAPD (Random Amplified Polymorphic DNA) (Williams et al 1990), SSR (Jacob et al 1991) e AFLP (Vos et al 1995) foram marcadores amplamente utilizados no melhoramento de plantas de diversas culturas (Cregan et al 1999a;Oliveira et al 2001;Cholastova et al 2011). Os primeiros mapas genéticos da soja, baseados em DNA, foram publicados na década de 90, sendo construídos inicialmente com marcadores RFLP, posteriormente também com marcadores AFLP, SSR e, recentemente, com a adição de milhares de marcadores SNP (Single Nucleotide Polymorphism), a partir das novas plataformas de sequenciamento e genotipagem (Keim et al 1990;Mansur et al 1993;Shoemaker and Specht 1995;Cregan et al 1999b;Song et al 2004;Wu et al 2004;Choi et al 2007;Hisano et al 2 007;Xia et al 2007;Hwang et al 2009;Hyten et al 2010;Niu et al 2013;Song et al 2013).…”

Section: Marcadores Moleculares Em Sojaunclassified

Identificação de QTLs em soja associados à resistência aos percevejos e a caracteres agronômicos utilizando a abordagem de mapeamento multivariado

Möller¹

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QTL identification in soybean related to stink bug resistance and agronomic traits using the multivariate multiple interval mapping approach Soybean is the Brazilian crop with the most expansion along the past three decades. Currently, it is a commodity commercialized in large scale as grain, bran and oil. Because it is an important source of protein, it plays a fundamental role in the food industry, both human and animal. Soybean monoculture in large areas has increased crop vulnerability to pathogens and insect pests, with significant consequences on grain production. Among such pest insects, stink bugs are considered a major pest of soybean crop, feeding directly on seeds, reducing yield and seed quality. Losses in crop yield due to stink bugs attack are greater than 30% and seed germination compromising can be greater than 50%. This study aimed to identify QTL (Quantitative Trait Loci), for stink bug resistance traits and agronomic traits using the multivariate multiple interval mapping. An F2 mapping population of 228 plants derived from a biparental cross between IAC-100 and CD-215 was used for genotyping. An F2:3 population was developed to evaluate eight stink bug resistant traits such as graining period, leaf retention, pod number per plant, percentage of pod damage, number of seeds, hundred seed weight, weight of healthy seeds and spotted seed weight. Other seven agronomic traits were evaluated such as number of days to flowering, plant height at flowering, number of days to maturity, plant height at maturity, lodging, agronomic value and grain yield. A total of 417 SNP (Single Nucleotide Polymorphism) markers, 61 SSR (Simple Sequence Repeat), 30 AFLP (Amplified Fragment Length Polymorphism) and 8 TRAP (Target Region Amplification Polymorphism) markers were mapped into 20 soybean linkage groups. The total map length was 2,814.82 cM with an average of 5.46 cM between markers. QTL mapping for those traits was performed using univariate (MIM) and multivariate (MT-MIM) multiple interval mapping, with main QTL effects estimates. MIM analysis identified a total of 60 QTL, through 13 soybean linkage groups, with 29 QTL related to stink bug resistant traits and 31 QTL related to agronomic traits. Phenotypic variation explained by QTL ranged from 14.27% for lodging to 65.45% for number of days to maturity. The traits were divided into nine groups for MT-MIM analysis considering their correlation coefficient. Twenty different genomic regions were identified showing a very high QTL clustering. For most of the traits phenotypic variation estimates for MT-MIM models were higher than MIM models, ranging from 27.98% to 65.30% for plant height at flowering and number of days to maturity, respectively. MT-MIM analysis showed 13 genomic regions controlling at least one of the evaluated traits which were not identified at MIM analysis. Comparison between identified QTL and QTL database at Soybase demonstrated that some QTL were similar to those described in different genetic background. However, 56 QTL detected in the pre...

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Marcadores Rapd Para Mapeamento Genético E Seleção De Híbridos De Citros

Cited by 5 publications

References 13 publications

Mapping of AFLP loci linked to tolerance to cowpea golden mosaic virus

Mapping of AFLP loci linked to tolerance to cowpea golden mosaic virus

Variability in Macadamia integrifolia by RAPD markers

Identificação de QTLs em soja associados à resistência aos percevejos e a caracteres agronômicos utilizando a abordagem de mapeamento multivariado

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