Solidifying agents and activated charcoal for in vitro culture of Solanum sessiliflorum

Rodrigues, Filipe Almendagna; Rezende, Renata Alves Lara Silva; Pasqual, Moacir; Lopes, Maria Teresa Gomes

doi:10.1590/s0100-204x2017001100019

Cited by 7 publications

(6 citation statements)

References 6 publications

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“…In the first experiment, in the evaluation 30 days after multiplication, there was no significant interaction by the F test for the amount of shoots, whole roots, broken roots, length of the larger shoots, fresh mass and broken roots mass. There was a significant difference between the concentrations of gelling agents only for the largest root length where treatment with Phytagel 2.5 g L -1 was statistically superior to treatment with Agar 4g L -1 (Figure 1), as well as the result found in the work of Rodrigues et al [15], where the researcher showed greater root length for the gelling agent Phytagel, in the cultivation of manna cubiu (Solanum sessiliflorum).…”

Section: Resultssupporting

confidence: 60%

“…According to the literature, the key gelling agents used for that context is Agar and Phytagel [13][14][15]. Agar is a hydrocolloid extracted from red seaweed and composed of two polysaccharides, agarose and agaropectin [13].…”

Section: Introductionmentioning

confidence: 99%

“…The concentration generally used for potato cultivation is 6 g L -1 of Agar [16]. Phytagel, a compound from the fermentation of bacteria, is considered superior to agar, as it is highly pure and translucent, which improves the production of plant shoots and reduces the cost of production [15]. Galactomannans are seed polysaccharides and despite having high viscosity it is not considered a gelling agent, however, has the potential to be used in micropropagation because they are non-toxic, stabilizers, thickeners and adhesives, controlling the viscosity of the solution [14].…”

Section: Introductionmentioning

confidence: 99%

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Gelling Agents and Partial Agar Replacement In Vitro for Production of Potato Plants

Ayub¹

2022

Arch Agri Res Technol

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The potato crop (Solanum tuberosum L.) is susceptible to pathogens that reduce its production. The micropropagation technique is used to reduce contaminants in seedlings, which requires gelling agents, with agar being the most used, which increases the cost of production. This study consists of two experiments: one with the cultivar Atlantic, under a completely randomized design, containing five treatments, with four replications, where three concentrations of Agar (6,5 and 4 gL-1) were tested, one concentration of Phytagel (2.5 gL-1) and the partial replacement of Agar adding Galactomannan (5+5 gL-1 ) and another with the Atlantic and Markies cultivars, under a completely causal experimental design, in a 2x5 factorial analysis, with the two cultivars and the same five types of gelling agents, containing 10 treatments, with four repetitions; 30 days after multiplication, morphometric evaluations were performed. After 30 days of acclimation, ex vitro survival, length of root, shoots and number of leaves were evaluated. For the statistics analysis, were used the R program and the variables compared using the Tukey test at 5 % of probability. It was concluded the partial replacement of Agar by Galactoman is possible, lowering the concentration of Agar to 5gL-1, where great results are obtained in the growth of explants, reducing the production cost in micropropagation and acclimatization of the cultivars Atlantic and Markies. There were no differences between agar at lower concentrations and the use of phytagel in the production of seedlings of cv.s Atlantic and Markies.

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Section: Resultssupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gelling Agents and Partial Agar Replacement In Vitro for Production of Potato Plants

Ayub¹

2022

Arch Agri Res Technol

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“…Conflicting data have been observed in previous studies regarding this point. While some researchers have reported higher regeneration frequencies on media solidified with gellan gum ( Mani et al, 2020 , Rodrigues et al, 2017 , Sun et al, 2008 ), other investigators have demonstrated the opposite ( Repalli et al, 2019 , Mitić et al, 2012 ). Combining both kinds of gelling agents (gellan gum and agar) proved to be the best ( Sah et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of different gelling agents on the different stages of rice regeneration in two rice cultivars

Mohamed

Amer

Osman

et al. 2021

Saudi Journal of Biological Sciences

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Plant tissue culture technology offers a solution for meeting the increasing commercial demand on economically important plants such as rice, a widespread dietary staple. However, significant genotype-specific morphogenetic responses constitute a considerable on rice regeneration in plant biotechnology contexts. Aside from genotype dependency, the components of the nutrient media including gelling agents have an important impact on regeneration efficiency. The current study explores the effect of different gelling agents on various stages of rice regeneration in two Egyptian rice cultivars-Sakha104 and Giza178. Media solidified with varying concentrations of a variety of gelling agents (agar, bacto agar, gelrite and phytagel) were tested for their impact on the frequency of callus induction, shoot regeneration and rooting. The results indicated gellan gum (gelrite and phytagel) was superior to agar products (agar and bacto agar) for callus induction. By contrast, no significant differences were found between different gelling agents for shoot regeneration. Gellan gum and media solidified with bacto agar were found to lead to significantly higher root regeneration than agar. The Sakha104 cultivar showed better responses than Giza 178 for callus induction and similar performance to the Giza 178 cultivar for root regeneration irrespective of the gelling agent. This work provides insights into the impact of different gelling agents on the morphogenetic response of two rice cultivars and can be used to help maximize the frequency of rice regeneration.

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“…Activated charcoal in the culture media can absorb substances, such as phenols and abscisic acid, which are inhibitory to microspore embryogenesis, from the environment of culture and agar, as well as ingredients produced by anther tissues (Öskum et al 2000;Kim et al 2019). It was reported, however, that activated charcoal had a negative impact on ferric EDTA, pyridoxine, biotin, thiamine, folic acid, nicotine acid, and hormonesupporting plant growth released from anther tissues and can absorb them from the culture medium (Fagundes et al 2017;Rodrigues et al 2017). In this study, the LG-7 line failed to develop plants from anthers cultured in the WAC medium, whereas transferring the cultured anthers after 35 d to the NAC medium resulted in 13 developed plantlets per 150 anthers.…”

Section: Discussionmentioning

confidence: 99%

Transferring the cultured anther to a medium without activated charcoal overcomes the recalcitrance in pepper genotypes

Pınar

Mutlu

Yıldız³

et al. 2021

Can. J. Plant Sci.

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Anther culture is a relatively easy and high-efficiency technique, however, low efficiency in plant regeneration may restrict its use in crop breeding. Activated charcoal is often used in in-vitro culture, and it may ameliorate or hinder in-vitro growth, depending on genotype and tissue used. Pepper is one of the main vegetable crops of the Solanaceae family, but some pepper genotypes are known to be recalcitrant to androgenesis and formation of haploid regenerants. Therefore, this study was aimed to explore the effect of activated charcoal on response to androgenesis in pepper genotypes. The plant material including 34 Long Green (LG), 13 Bell pepper (BP), 13 Charleston (Ch), 6 California Wonder (CW), and 23 Capia (CP) advanced breeding lines. Initially, anthers were cultured in a medium with activated charcoal (WAC) for 25, 35, or 45 days, and then they were transferred to the same medium without activated charcoal (NAC). In the WAC medium, 15 lines of LG genotype showed the highest recalcitrancy while many lines of CW had the lowest recalcitrancy to androgenesis. However, after transferring the 35-day old anthers to a NAC medium, the androgenesis was observed in recalcitrant LG lines. The results indicated that transferring the cultured anthers from WAC medium, ideally after 35-day, to a NAC medium overcame the recalcitrancy to androgenesis in pepper.

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Solidifying agents and activated charcoal for in vitro culture of Solanum sessiliflorum

Cited by 7 publications

References 6 publications

Gelling Agents and Partial Agar Replacement In Vitro for Production of Potato Plants

Gelling Agents and Partial Agar Replacement In Vitro for Production of Potato Plants

Effects of different gelling agents on the different stages of rice regeneration in two rice cultivars

Transferring the cultured anther to a medium without activated charcoal overcomes the recalcitrance in pepper genotypes

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