Effects of different gelling agents on the different stages of rice regeneration in two rice cultivars

Abstract

Plant tissue culture technology offers a solution for meeting the increasing commercial demand on economically important plants such as rice, a widespread dietary staple. However, significant genotype-specific morphogenetic responses constitute a considerable on rice regeneration in plant biotechnology contexts. Aside from genotype dependency, the components of the nutrient media including gelling agents have an important impact on regeneration efficiency. The current study explores the effect of different gelling agents on various stages of rice regeneration in two Egyptian rice cultivars-Sakha104 and Giza178. Media solidified with varying concentrations of a variety of gelling agents (agar, bacto agar, gelrite and phytagel) were tested for their impact on the frequency of callus induction, shoot regeneration and rooting. The results indicated gellan gum (gelrite and phytagel) was superior to agar products (agar and bacto agar) for callus induction. By contrast, no significant differences were found between different gelling agents for shoot regeneration. Gellan gum and media solidified with bacto agar were found to lead to significantly higher root regeneration than agar. The Sakha104 cultivar showed better responses than Giza 178 for callus induction and similar performance to the Giza 178 cultivar for root regeneration irrespective of the gelling agent. This work provides insights into the impact of different gelling agents on the morphogenetic response of two rice cultivars and can be used to help maximize the frequency of rice regeneration.

Developed applicability of a bacterial cellulose matrix as a gelling substitute for plant tissue culture media

“…Studies were also extended to investigate a wide range of plant species and crops in an attempt to screen the best ingredients to be used in explants and medium components. Our study has also produced convincing results consistent with those obtained with bananas (Kacar et al 2010), wheat (Malik et al 2017), kiwi (Mardiana et al 2018), oil and date palms (Al-Mayahi and Ali 2021; Palanyandy et al 2020), and rice (Gehad M. Mohamed et al 2021;Repalli et al 2019).…”

“…alternative gelling agents such as gelrite (Gehad M Mohamed et al 2021), gum katira (Jain and Babbar 2002), isubgol and sago starch (Bhattacharya et al 1994), guar gum (Babbar et al 2005), locust bean gum (LBG) (Gonçalves and Romano 2005), and xanthan gum (Jain and Babbar 2006). Consequently, this has widened the applicability of plant tissue culture techniques as an effective tool in biotechnology.…”

The Effect of Silver Nanoparticle Addition on Micropropagation of Apricot Cultivars (Prunus armeniaca L.) in Semisolid and Liquid Media

“…The type and concentration of gelling agent may be essential for the use of AgNPs in a semisolid medium. Phytagel TM has macropores with the size between 100 and 500 µm [ 56 ], and has been described to encourage faster plant growth, as compared with agar [ 57 ].…”

An Efficient Agrobacterium-Mediated Transformation Method for Hybrid Poplar 84K (Populus alba × P. glandulosa) Using Calli as Explants

“…Phytagel addition induced the formation of a large number of healthy calli (Table 1) with a higher shoot induction frequency than agar (Table 2), making it suitable for the establishment of a high-throughput transformation system. Gelling agents (phytagel or agar) affect the chemical and physical characteristics of the culture medium, such as the diffusion rate of nutrients, elemental and organic impurities and gel strength, which may influence the effect on callus induction and shoot induction [46,47].…”