Different extenders solutions for tambaqui semen cooling

Garcia, Raycon Roberto Freitas; Vasconcelos, Ana Carina Nogueira; Povh, Jayme Aparecido; Oberst, Eneder Rosana; Eloy, Lidiane Raquel; Streit, Danilo Pedro

doi:10.1590/s0100-204x2016000600011

Cited by 7 publications

(3 citation statements)

References 10 publications

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“…In this study, a 1:4 (sperm: extender) dilution ratio was superior to 1:1, 1:2, and 1:10 ratios in terms of sustaining motility. Similar results have been reported for meagre (Argyrosomus regius) [24], tambaqui (Colossoma macropopum) [27], walking catfish (Clarias macrocephalus) [28], and starry flounder (Platichthys stellatus) [7]. The optimum dilution ratio for maintaining motility differs according to fish species.…”

Section: Discussionsupporting

confidence: 82%

Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality

et al. 2023

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Intermediate-term preservation of sperm assists the reproductive management of fish spermatozoa; however, no information is available on sperm of the spotted halibut, Verasper variegatus. We aimed to identify the optimum diluents, temperatures, dilution ratios, antibiotics, and antioxidants for sperm motility and cell viability. The diluents evaluated were marine fish Ringer’s solution (MFRS), Stein’s solution, 300 mM sucrose, and 300 mM glucose (diluted 1:1 [sperm: diluent], 1:2, 1:4, and 1:10 and stored at 0, 2, 4, and 6 °C). Neomycin and gentamycin (100, 200, 400, and 800 mg/L) and antioxidants (Mito-TEMPO [0, 25, 50, 75, 100, 125, 150, 175, and 200 µM], reduced glutathione [0, 2, 4, 6, 8, and 10 mM], and trehalose [0, 50, 100, 150, 200, and 250 mM]) were assessed in terms of sperm preservation. The most effective condition for cold storage of spotted halibut sperm was Stein’s solution at a dilution ratio of 1:4 at 2 °C, with a combination of neomycin 800 mg/L and 250 mM trehalose that showed spermatozoa motility of >43% after 60 days. These storage conditions will be valuable for spotted halibut hatcheries.

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Section: Discussionsupporting

confidence: 82%

Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality

et al. 2023

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“…These extenders are composed of standardized in natura coconut water and sugar, proteins, vitamins, amino acids, minerals, fat, and pH regulators (glycine and sodium citrate). Another extender widely used to cool swine semen is BTS (Beltsville Thawing Solution), which does not contain coconut water in its formulation, and is composed of sodium citrate, sodium bicarbonate, EDTA, potassium chloride, streptomycin, and glucose, and has been used in the cooling of semen of other species (Garcia et al 2016, Zorinkimi et al 2017.…”

Section: Introductionmentioning

confidence: 99%

Use of the ACP® and BTS extenders for cooling at 15°C white-lipped peccary (Tayassu pecari) semen

et al. 2019

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Knowledge about reproduction of white-lipped peccary is of great importance to assist with the conservation of this species and enable its rational use in captivity. This study aimed to evaluate the effect of ACP-103®, ACP-116® and BTS semen extenders on sperm viability during cooling of Tayassu pecari semen. Five ejaculates from four adult males were chilled. The animals were submitted to the protocols of sedation and anesthesia for semen collection by the electroejaculation method. After collection, the semen was macro- and microscopically assessed and diluted to reach 35x106 spermatozoa/mL in each of the three different extenders tested. The fresh-extended semen was packed in a BotuFLEX® thermal box to keep samples at 15°C for 24 hours. After cooling, the following semen parameters were analyzed: sperm motility, functional and structural integrity of sperm membranes, mitochondrial activity, chromatin condensation, and the thermoresistance test was performed. The parameters sperm motility, structural and functional integrity of sperm membranes, mitochondrial activity, and chromatin condensation were preserved after use of the extenders tested, and were similar to those of in natura semen (p>0.05). Curvilinear velocity (VCL) (p<0.05) was the only parameter with reduced values after cooling regardless of the extender used. The percentage of sperm with normal morphology was greater in samples cooled using the BTS extender (p<0.05). The ACP-103®, ACP-116® and BTS extenders can be used for the cooling and preservation of white-lipped peccary semen at 15°C for 24 hours.

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“…This practice allows the stored semen to fertilize the oocytes after a longer time, compared to fresh semen. Cold storage of semen retains the motility time significantly longer than room temperature storage, thus ensuring higher efficacy of reproduction, cost reduction (Garcia et al, 2016;Marques & Godinho, 2004;Oliveira et al, 2012) and ease of transportation (Carneiro, Segui, Ióris Filho, & Mikos, 2006;Davy & Chouinard, 1980;Henderson & Dewar, 1959).…”

Section: Introductionmentioning

confidence: 99%

Sperm quality of Colossoma macropomum after room‐temperature and cold storage

Pires

Sanches

Romagosa³

et al. 2019

J Appl Ichthyol

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Summary The objective of this study was to evaluate the effects of cold and room‐temperature storage on the quality of Colossoma macropomum sperm. The experiment was carried out in December (end of Spring), in Nova Mutum‐MT, Brazil, involving nine C. macropomum males (4 years old; 6.4 ± 1.5 kg average weight). The fish were selected and transferred to masonry tanks (4 m3) in a laboratory (water renewal rate: 10 L/s; average water temperature: 28°C). Subsequently, reproduction was induced using 2.5 mg of crude carp pituitary extract/kg and the semen was harvested 240 degree hours after hormonal induction. The following sperm characteristics were analyzed every 5 hr using ImageJ/casa software: total motility (MOT), curvilinear velocity (VCL), average path velocity (VAP), straight‐line velocity (VSL), straightness of sperm path (STR), wobble (WOB), progressive motility (PROG), beat cross frequency (BCF) and total number of spermatozoa (NSPZ). A fresh sample of semen from each animal was kept at room temperature (25.3 ± 1.2°C). For analysis of cooled semen, syringes were kept in cooling boxes at an average temperature of 16.9 ± 2.1°C. The reduction (p < 0.05) of MOT in semen kept at room temperature occurred at 10 hr (13.95%); in cooled semen, however, MOT declined at 15 hr (76.87%). At 15 hr, there was practically no MOT in the semen kept at room temperature (0.20%), whereas in the cooled semen this situation was observed only at 35 hr (2.91%) The MOT of cooled sperm was higher (p < 0.05) at all times (except zero time), compared with the semen maintained at room temperature. At 15 hr, the cooled spermatozoa showed higher (p < 0.05) VCL (142.18 μm/s) and BCF (29.72 Hz) than those maintained at room temperature (VCL: 51.18 μm/s; BCF: 19.57 Hz). After 15 hr, only the cooled sperm showed quality. In conclusion, semen cooling allows for extending the viability of C. macropomum spermatozoa from 5 to 10 hr without compromising their quality in most characteristics. At 15 and 25 hr of cooling, sperm viability is still observed, though with decreased quality.

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Different extenders solutions for tambaqui semen cooling

Cited by 7 publications

References 10 publications

Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality

Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality

Use of the ACP® and BTS extenders for cooling at 15°C white-lipped peccary (Tayassu pecari) semen

Sperm quality of Colossoma macropomum after room‐temperature and cold storage

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