Genetic variability of hull-less barley accessions based on molecular and quantitative data

Abstract

-The objective of this work was to characterize and quantify the genetic, molecular, and agronomic variability of hull-less barley genotypes, for the selection of parents and identification of genotypes adapted to the irrigated production system in the Brazilian Cerrado. Eighteen hull-less barley accessions were evaluated, and three covered barley accessions served as reference. The characterization was based on 157 RAPD molecular markers and ten agronomic traits. Genetic distance matrices were obtained based on molecular markers and quantitative traits. Graphic grouping and dispersion analyses were performed. Genetic, molecular, and agronomic variability was high among genotypes. Ethiopian accessions were genetically more similar, and the Brazilian ones were genetically more distant. For agronomic traits, two more consistent groupings were obtained, one with the most two-rowed materials, and the other with six-rowed materials. The more diverging materials were the two-rowed CI 13453, CN Cerrado 5, CN Cerrado 1, and CN Cerrado 2. The PI 356466, CN Cerrado 1, PI 370799, and CI 13453 genotypes show agronomic traits of interest and, as genetically different genotypes, they are indicated for crossing, in breeding programs.Index terms: Hordeum vulgare, genetic diversity, molecular markers, quantitative traits. Variabilidade genética de acessos de cevada-nua com base em dados moleculares e quantitativosResumo -O objetivo deste trabalho foi caracterizar e quantificar a variabilidade genética, molecular e agronômica de genótipos de cevada-nua, para a seleção de genitores e a identificação de genótipos adaptados ao sistema de produção irrigada no bioma Cerrado. Dezoito acessos de cevada-nua foram avaliados e três acessos de grãos de cevada com casca serviram como referência. A caracterização baseou-se em 157 marcadores moleculares RAPD e dez características agronômicas. As matrizes de distância genética foram obtidas com base em marcadores moleculares e características quantitativas. Realizaram-se análises de agrupamento e dispersão gráfica dos acessos. A variabilidade genética, molecular e agronômica foi alta entre os acessos. Os acessos etíopes foram geneticamente mais similares, e os acessos brasileiros, geneticamente mais distantes. Quanto às características agronômicas, obtiveram-se dois agrupamentos mais consistentes, um com mais materiais dísticos e outro com os hexásticos. Os genótipos mais divergentes foram os dísticos CI 13453, CN Cerrado 5, CN Cerrado 1 e CN Cerrado 2. Os genótipos PI 356466, CN Cerrado 1, PI 370799 e CI 13453 apresentam características agronômicas de interesse e, como são distintos geneticamente, são indicados para cruzamentos em programas de melhoramento genético.Termos para indexação: Hordeum vulgare, diversidade genética, marcador molecular, caracteres quantitativos.

Genetic parameters and agronomic characterization of elite barley accessions under irrigation in the Cerrado

“…These studies are important for obtaining a wider knowledge of elite and promising barley in irrigated cultivation in the Cerrado. For the characteristics TSE, Height and DH, the CEV values were close to those obtained by Sayd et al (2017) and Sayd, Amabile, Faleiro, and Bellon (2015), who only collected the parameters at different sites.…”

Molecular and agronomic genetic diversity between barley genotypes under irrigation in the Brazilian Cerrado

“…In another study, eight genotypes from various regions of the world showed 0.61 mean genetic dissimilarity (Drine et al, 2016). An evaluation using RAPD markers, in the Brazilian Cerrado, showed 0.28 mean genetic dissimilarity for 21 naked barley genotypes from different continents (Sayd et al, 2015). In this scenario, the genetic dissimilarity values obtained in the present study show that the genotypes have a considerable degree of variability, although most of them are of Colombian origin and have been selected for the same agronomic characteristics.…”

Genetic parameters and morpho-agronomic characterization of barley in the Brazilian Savannah

“…Characterization of large numbers of test samples is restricted by labour costs, the area required, financial resources and the number of seeds. Molecular markers have been used to characterize barley accessions however these tools cannot replace morpho‐agronomic characterization. The use of cost‐effective elaborate designs to reduce experimental error for morpho‐agronomic characterization has been limited.…”