Evaluation of acid digestion techniques to estimate chromium contents in cattle feces

Rocha, Gabriel Cipriano; Palma, Malber Nathan Nobre; Detmann, Edênio; Filho, Sebastião de Campos Valadares

doi:10.1590/s0100-204x2015000100010

Cited by 5 publications

(6 citation statements)

References 4 publications

(3 reference statements)

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“…It is significant that regardless of sample or solution ratio, one-step digestion can be used for all the minerals. Previous work in our laboratory showed a similar pattern for chromium analysis ( Rocha et al, 2015 ).…”

Section: Discussionsupporting

confidence: 78%

Evaluation of Acid Digestion Procedures to Estimate Mineral Contents in Materials from Animal Trials

Palma¹,

Rocha²,

Filho³

et al. 2015

Asian Australas. J. Anim. Sci

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Rigorously standardized laboratory protocols are essential for meaningful comparison of data from multiple sites. Considering that interactions of minerals with organic matrices may vary depending on the material nature, there could be peculiar demands for each material with respect to digestion procedure. Acid digestion procedures were evaluated using different nitric to perchloric acid ratios and one- or two-step digestion to estimate the concentration of calcium, phosphorus, magnesium, and zinc in samples of carcass, bone, excreta, concentrate, forage, and feces. Six procedures were evaluated: ratio of nitric to perchloric acid at 2:1, 3:1, and 4:1 v/v in a one- or two-step digestion. There were no direct or interaction effects (p>0.01) of nitric to perchloric acid ratio or number of digestion steps on magnesium and zinc contents. Calcium and phosphorus contents presented a significant (p<0.01) interaction between sample type and nitric to perchloric acid ratio. Digestion solution of 2:1 v/v provided greater (p<0.01) recovery of calcium and phosphorus from bone samples than 3:1 and 4:1 v/v ratio. Different acid ratios did not affect (p>0.01) calcium or phosphorus contents in carcass, excreta, concentrate, forage, and feces. Number of digestion steps did not affect mineral content (p>0.01). Estimated concentration of calcium, phosphorus, magnesium, and zinc in carcass, excreta, concentrated, forage, and feces samples can be performed using digestion solution of nitric to perchloric acid 4:1 v/v in a one-step digestion. However, samples of bones demand a stronger digestion solution to analyze the mineral contents, which is represented by an increased proportion of perchloric acid, being recommended a digestion solution of nitric to perchloric acid 2:1 v/v in a one-step digestion.

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Section: Discussionsupporting

confidence: 78%

Evaluation of Acid Digestion Procedures to Estimate Mineral Contents in Materials from Animal Trials

Palma¹,

Rocha²,

Filho³

et al. 2015

Asian Australas. J. Anim. Sci

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show abstract

“…The chromium concentration in the feces samples was assessed through atomic absorption spectrophotometry (GBC Avanta Σ, Scientific Equipment, Braeside, Victoria, Australia) using digestion techniques with nitric and perchloric acids, at the ratio of 3:1 v v −1 , in one-step digestion with sodium molybdate as catalyst [ 17 ]. The concentration of titanium dioxide in the fecal samples was evaluated by spectrophotometry (INCT-CA; method M-007/2).…”

Section: Methodsmentioning

confidence: 99%

Periparturient Changes in Voluntary Intake, Digestibility, and Performance of Grazing Zebu Beef Cows with or without Protein Supplementation

David,

Matos,

Domingos

et al. 2024

Animals

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We aimed to understand the changes in nutritional parameters and performance of beef cows during the peripartum, whether receiving or not receiving protein supplements. Forty cows were used, divided into two treatments: CON—mineral mix and SUP—protein supplementation. Digestibility trial was performed at 45, 30, and 15 days (d) before the parturition and at 20 and 40 d of lactation. The ADG of cows pre- and postpartum was recorded along with the BCS in gestational (GT) and maternal (MT) tissues in the prepartum. There was an effect of treatment and period (p ≤ 0.044) for intakes of DM and CP. The forage intake was similar (p > 0.90) but varied with the effect of days related to parturition (p < 0.001). There was a 14.37% decrease in DM intake from d −30 to d −15 of prepartum. In the postpartum, at 20 d of lactation, there was an increase of 72.7% in relation to d −15 of prepartum. No differences were observed in postpartum ADG or BCS at parturition and postpartum (p ≥ 0.12). However, higher total and MT ADG (p ≤ 0.02) were observed in animals receiving supplementation, while ADG in GT remained similar (p > 0.14). In conclusion, there is a decrease in intake for pregnant cows close to parturition and greater performance of animals supplemented in prepartum.

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“…The estimation of iNDF contents of the samples, processed to pass through a 2-mm screen siev, was performed according to Valente et al (2011), in a 288-h in situ incubation procedure. The chromium concentration in the feces samples was determined with atomic absorption spectrophotometry (GBC Avanta Σ, Scienti c Equipment, Braeside, Victoria, Australia) using digestion techniques with nitric and perchloric acids, at the ratio of 3:1 v v -1 , in one-step digestion with sodium molybdate as catalyst (Rocha et al, 2015). The concentration of titanium dioxide in the fecal samples was determined by spectrophotometry (INCT-CA; method M-007/2).…”

Section: Laboratory Analyzes and Calculationsmentioning

confidence: 99%

Changes in nutritional characteristics and performance of grazing Nellore cows during the peripartum phase receiving or not protein supplementation

David

Matos

Domingos

et al. 2022

Preprint

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We objective was to understand the changes in nutritional characteristics and performance of grazing Nellore cows during the peripartum receiving or not supplementation. Forty multiparous cows were used, divided into two treatments: CON - mineral mixture and SUP – protein supplementation. Digestibility trial was performed (-45,-30,-15, + 20, +40; days relative to calving). The average daily gain (ADG) pre and postpartum of the cows were measured and, in gestational and maternal tissues in the prepartum, in addition of body condition score (BCS). The calves were weighed at birth and at 30 days of age. There was an effect of treatment and period (P ≤ 0.10) for dry matter, organic matter and crude protein intake, while forage intake was similar (P ≥ 0.10), but with a period effect (P ≤ 0.10). There was 14.37% decrease in dry matter intake from day − 30 to day − 15 of prepartum. In the postpartum period, at 20 days of lactation, there was increase of 72.66% in relation to the period − 15. There was interaction between period and treatment for all digestibilities (P ≤ 0.10). No differences were observed in postpartum ADG (P ≥ 0.10), BCS at calving and postpartum (P ≥ 0.10). However, higher total ADG and in maternal tissues (P ≤ 0.10) were observed on supplemented animals, but with ADG in gestational tissues similar (P ≥ 0.10). It is concluded that there is a decrease in voluntary intake in grazing pregnant cows close to parturition and greater performance of animals supplemented in prepartum.

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Evaluation of acid digestion techniques to estimate chromium contents in cattle feces

Cited by 5 publications

References 4 publications

Evaluation of Acid Digestion Procedures to Estimate Mineral Contents in Materials from Animal Trials

Evaluation of Acid Digestion Procedures to Estimate Mineral Contents in Materials from Animal Trials

Periparturient Changes in Voluntary Intake, Digestibility, and Performance of Grazing Zebu Beef Cows with or without Protein Supplementation

Changes in nutritional characteristics and performance of grazing Nellore cows during the peripartum phase receiving or not protein supplementation

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