Micropropagação de sucupira-preta por meio de gemas axilares

Abstract

Resumo -O objetivo deste trabalho foi avaliar o uso de reguladores de crescimento, aditivos antioxidantes, tipo de explantes e intensidade de cobertura de mudas aclimatizadas, na micropropagação de sucupira-preta (Bowdichia virgilioides) por meio de gemas axilares. Os explantes foram cultivados para a multiplicação em meio de cultura básico WPM, suplementado com concentrações de benzilaminopurina (BAP). No alongamento, os tratamentos foram combinações de ácido naftalenoacético (ANA) e BAP adicionadas ao meio. Para o enraizamento, as brotações foram colocadas em meio com concentrações de ácido indolbutírico, ou em meio com combinações dos aditivos polivinilpirrolidona e carvão ativado, em diferentes concentrações de ANA. As plantas foram transplantadas para copos de plástico, com substrato, e cobertas com saco de polietileno. Posteriormente, esses sacos foram retirados, ou perfurados, ou não retirados, o que constituiu os tratamentos de pré-aclimatização in vitro. A aclimatização ex vitro foi realizada após o período de pré-aclimatização in vitro. A sucupira-preta apresentou melhor micropropagação com emprego de: segmentos cotiledonares e 0,3 mg L -1 de BAP, na multiplicação; 0,3 mg L -1 de ANA e 0,03 mg L -1 de BAP, no alongamento; e carvão ativado (2,0 mg L -1 ), no enraizamento e na pré-aclimatização in vitro, com uso de cobertura de plástico transparente em torno da muda.Termos para indexação: Bowdichia virgilioides, cultura de tecidos, espécie nativa, propagação, reguladores de crescimento. Sucupira-preta micropropagation through axillary budsAbstract -The objective of this work was to evaluate the use of growth regulators, antioxidant additives, type of explants, and coverage intensity of acclimatized seedlings in the micropropagation of sucupira-preta (Bowdichia virgilioides) through axillary buds. Explants were cultured for multiplication on WPM medium, supplemented with concentrations of benzylaminopurine (BAP). In the elongation phase, treatments consisted of naphthaleneacetic acid (NAA) and BAP combinations added to the medium. For rooting, shoots were inoculated in medium containing concentrations of indolbutyric acid or combinations of the additives polyvinylpyrrolidone and charcoal, in different NAA concentrations. Plants were transplanted to plastic cups with substrate, and covered with polyethylene bags. Later, the bags were withdrawn, or perforated, or not withdrawn, which constituted the in vitro pre-acclimatization treatments. Ex vitro acclimatization was carried out after the period of in vitro pre-acclimatization. Sucupira-preta had the best micropropagation using: cotyledon segments and 0.3 mg L -1 BAP, for multiplication; 0.3 mg L -1 NAA and 0.03 mg L -1 BAP, for elongation; and 2,0 mg L -1 activated carbon for rooting and for the in vitro pre-acclimatization, with the use of a transparent plastic cover around seedling.Index terms: Bowdichia virgilioides, tissue culture, native species, propagation, growth regulators. IntroduçãoA sucupira-preta (Bowdichia virgilioides Kunth.) é uma espécie ...

In vitro propagation and acclimatization of genipapo accessions

“…An increasing trend in the percentage of callus formation in axillary buds of black sucupira (Bowdichia virgilioides Kunth.) with an increase in IBA concentration were observed by Moura et al (2012). The presence of callus in the rooting area may affect the root quality, especially regarding the vascular connection with the plant De Klerk, 2008b).…”

“…However, the explants showed similar heights that were lower than 1.53 cm in the absence of BAP in the culture media. Cytokinins are a class of plant-growth regulators able to induce cell division (Nishimura et al, 2004) and are responsible for the formation of organs, especially plant shoot organs (Moura et al, 2012).The superiority of the cultures maintained in the presence of 1.0 mg L -1 BAP is evidenced by greater leaf number, adventitious shoots and shoot length when compared with those of the plants grown in the absence of cytokinin.…”

Productivity of Rhizomes and Starch Quantification in Cultures of Different Vegetative Propagules of Arrowroot

“…In tissue culture is a strategy that allows the rapid multiplication of the material to be propagated from a single individual, and can be done at any time of the year, allowing a greater control over the sanity of the material, and this technique is important because it can be used for the production of endangered native species (Campos et al, 2013;Thakur & Karnosky, 2007). With micropropagation it is possible to maximize the quality and uniformity of the plantations when selected genotypes are used (Moura et al, 2012).…”

Bioquímica do estresse, histoquímica e aspectos ultraestruturais de calos de teca (<i>Tectona grandis</i> L.f.) visando a indução da embriogênese somática

“…Com uma capacidade marcante no processo de divisão celular estão os reguladores de crescimento denominados citocininas (MOURA et al, 2012). Para Hall (1975), as citocininas são substâncias que promovem o crescimento e a diferenciação em cultura de calo.…”