Vitamin C and endogenous cortisol in foreign-body inflammatory response in pacus

Belo, Marco Antônio de Andrade; Moraes, Julieta Rodini Engrácia de; Soares, Vando Edésio; Martins, Maurício Laterça; Brum, Camila Dantas; Moraes, Flávio Ruas de

doi:10.1590/s0100-204x2012000700019

Cited by 36 publications

(37 citation statements)

References 24 publications

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“…However, the development of colonies may result in local irritation, since multifocal granulomatous dermatitis was found in infested fish, characteristic of a chronic pathological process (Ulrich et al, 2007). Mononuclear infiltration and giant cell formation have also been reported in the evolution of chronic inflammatory response in teleost fish (Belo et al, 2005(Belo et al, , 2012Sakabe et al, 2013), including peritrich infestation (Valladão et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

Host-parasite relationship during Epistylis sp. (Ciliophora: Epistylididae) infestation in farmed cichlid and pimelodid fish

Pádua¹,

Martins

Valladão

et al. 2016

Pesq. agropec. bras.

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-The objective of this work was to describe the host-Epistylis sp. relationship during infestation on farmed fish. Five Nile tilapia (Oreochromis niloticus) and ten hybrid surubim catfish (Pseudoplatystoma reticulatum x P. corruscans), all diseased, were used for in vivo morphological analysis of sessile peritrichs by contrast microscopy. Fragments of infected tissues were subjected to histological processing and scanning electron microscopy. Epistylis sp. caused hemorrhagic ulcer disease, and cichlids were more prone to develop infestations throughout the body surface due to the attachment of the colonies to the scales, which did not occur with pimelodids. Multifocal granulomatous dermatitis was observed, associated with the hydropic degeneration of the epithelium and to ulcerative areas of necrosis. Microscopic examination showed the presence of bacterial microflora associated to Epistylis sp. peduncles. Therefore, this species can be considered a portal of entry for opportunistic bacteria.Index terms: Oreochromis niloticus, Pseudoplatystoma reticulatum, Pseudoplatystoma corruscans, histopathology, pathogenesis, sessile peritrich. Relação hospedeiro-parasita durante infestação por Epistylis sp. (Ciliophora: Epistylididae) em peixes ciclídeos e pimelodídeos cultivadosResumo -O objetivo deste trabalho foi descrever a relação hospedeiro-Epistylis sp. durante infestação em peixes cultivados. Foram utilizados cinco tilápias-do-nilo (Oreochromis niloticus) e dez híbridos de surubim (Pseudoplatystoma reticulatum x P. corruscans), todos parasitados, para análise morfológica in vivo de peritríquios sésseis por microscopia de contraste de fase. Fragmentos de tecidos infectados foram processados para histologia e microscopia eletrônica de varredura. Epistylis sp. causou doença ulcerativa hemorrágica, e os ciclídeos foram mais susceptíveis ao parasitismo na superfície do corpo em razão da fixação das colônias nas escamas, o que não ocorreu nos pimelodídeos. Foi observada ocorrência de dermatite granulomatosa multifocal, associada à degeneração hidrópica do epitélio e às áreas ulcerativas de necrose. O estudo microscópico mostrou a presença de bactérias associadas aos pedúnculos de Epistylis sp. Portanto, esta espécie pode ser considerada porta de entrada para bactérias oportunistas.

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Section: Resultsmentioning

confidence: 99%

Host-parasite relationship during Epistylis sp. (Ciliophora: Epistylididae) infestation in farmed cichlid and pimelodid fish

Pádua¹,

Martins

Valladão

et al. 2016

Pesq. agropec. bras.

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“…Moreover, the high concentration of glucocorticoids due to handling stress and treatment could favor energy metabolism by stimulating gluconeogenesis, increasing the bioavailability of free fatty acids resulting from the breakdown of triglycerides [22,23]. Sakabe et al [24], Belo et al [25,26] and Castro et al [27] reported the occurrence of stress in animals, resulting of handling and treatment. According to these authors, the organic response to stress conditions is complex, involving neuro-endocrine mechanisms with the release of glucocorticoids and catecholamines which act by increasing the metabolic activity and consequently altering the organic balance.…”

Section: Discussionmentioning

confidence: 99%

Clinical safety of amino acids and vitamins administered through parenteral route

Moraes¹,

Scherma²,

Prado³

et al. 2017

Int J Vet Sci Res

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The parenteral use of amino acids and vitamins has been used to improve performance in animal production. However, few studies demonstrating the toxicity of these compounds. The present investigation evaluated the toxicological and clinical safety of the injectable supplement compound by amino acids and vitamins, administered by intramuscular route in Wistar rats. 56 females (± 220g), pubescent virgins, were randomly divided into seven groups (n=8): Treated with dose of 0.2, 2 and 4 mL, sampled 24 and 96 hours post-treatment (HPT), totaling six groups and one control group (Physiology Standard). Signifi cant increase in the enzymatic activity of serum ALT, AST, LDH and ALP was observed in animals treated with 2 and 4 mL (24 HPT). However, these changes returned to baseline levels 96 HPT, except in serum AST and ALP in animals treated with 4 mL. CK-NAC showed dose-response effect, since the higher compound dose was followed by proportional increase in the circulating levels of this enzyme, associated to muscle degeneration and necrosis area in rats treat with 4mL at 96 HPT. The evaluation of hepatic function did not result in signifi cant changes in the serum levels of triglycerides, cholesterol, albumin, urea and creatinine in the comparison between rats treated with 0.2 mL and control rats, demonstrating the innocuousness of this treatment on the activity of liver and kidney. However, deleterious effects were observed in animals subjected to doses of 2 and 4 mL, including observed increased splenic and hepatosomatic response, signifi cant decrease in circulating albumin levels and signifi cant leukocytosis, which is characterized by neutrophilia, followed by lymphopenia demonstrating the compound irritating effect on muscle tissue.

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“…The pacu (Piaractus mesopotamicus), an emergent species in the world aquaculture and considered a good experimental model, is a native teleost fish of the Parana--Paraguay Basin, and is of importance in the South America for human consumption, angling and aquaculture (Belo et al 2005(Belo et al , 2012. The pacu has proven to be a good bioindicator of water quality, and in accordance with Castro et al (2014b) this species has been used in ecotoxicity studies for registration of chemicals in Brazil, and no information about the effects caused by the use of anticoagulants on the hematological and biochemical parameters has been related in the literature.…”

Section: Introductionmentioning

confidence: 99%

Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus

Farias¹,

Pereira²,

Pádua³

et al. 2016

Pesq. Vet. Bras.

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RESUMO.-[Anticoagulante Na 2 EDTA danifica amostras de sangue do teleósteo Piaractus mesopotamicus.] O presente estudo teve por objetivo avaliar os efeitos da heparina sódica e Na 2 EDTA no sangue de Piaractus mesopotamicus (360,7±42,4g, 26,4±1,0cm). Foram amostrados vinte peixes em dois ensaios experimentais, sendo dez peixes utilizados para análise da fragilidade dos eritrócitos e dez peixes para análise dos parâmetros hematológicos e estudo bioquímico do plasma. O sangue coletado por punção veno-caudal foi aliquotado e armazenado em diferentes soluções anticoagulantes: Na 2 EDTA 10%, Na 2 EDTA 3%, heparina sódica 5.000 UI e heparina sódica 100 UI. Níveis plasmáticos de cálcio apresentados nas amostras armazenados em Na 2 EDTA diminuíram cerca de 80% em relação aos dois grupos armazenados com heparina. Amostras de sangue de pacus armazenados com Na 2 EDTA demonstraram aumento do hematócrito e VCM, e diminuição na CHCM. O efeito dose-resposta foi observado neste estudo. Estes resultados são reforçados pelos níveis mais elevados de proteína plasmática e hemólise apresentado no sangue armazenado com Na 2 EDTA 10%, o que confirma o efeito deletério desse tratamento anticoagulante na qualidade de amostras de sangue. Na 2 EDTA não é indicada para armazenar amostras de sangue de P. mesopotamicus, e heparina sódica a 100 UI é o anticoagulante mais recomendado, uma vez que este tratamento apresentou a menor taxa de alterações no sangue armazenado. The present study aimed to evaluate the effects of Na heparin and Na 2 EDTA on blood of Piaractus mesopotamicus (360.7±42.4g, 26.4±1.0cm). Twenty fishes were sampled in two experiment trials, ten for erythrocyte fragility analysis and ten for hematologic and plasma biochemical study. The blood collected by venous-caudal puncture was fractioned and stored in anticoagulants solution: Na 2 EDTA 10%, Na 2 EDTA 3%, Na heparin 5000 IU and Na heparin 100 IU. Plasmatic levels of calcium presented in the Na 2 EDTA stored samples were about 80% lower than both heparin groups. Blood samples of P. mesopotamicus stored with Na 2 EDTA demonstrated increase in the hematocrit and MCV, and decrease in MCHC. The dose-response effect was observed in this study. The results are reinforced by the higher levels of plasmatic protein and hemolysis presented in the Na 2 EDTA 10% stored blood, confirming the deleterious effect of this anticoagulant treatment on the quality of blood samples. Na 2 EDTA is not indicated to store P. mesopotamicus blood samples, but sodium heparin at 100 IU is the most recommended anticoagulant, since this treatment presented the lower rate of alterations in the stored blood. INDEX TERMS: Fish hematology, blood clotting, EDTA, Heparin, Piaractus mesopotamicus.

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Vitamin C and endogenous cortisol in foreign-body inflammatory response in pacus

Cited by 36 publications

References 24 publications

Host-parasite relationship during Epistylis sp. (Ciliophora: Epistylididae) infestation in farmed cichlid and pimelodid fish

Host-parasite relationship during Epistylis sp. (Ciliophora: Epistylididae) infestation in farmed cichlid and pimelodid fish

Clinical safety of amino acids and vitamins administered through parenteral route

Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus

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