Genetic variation and environmental effects on beta-conglycinin and glycinin content in Brazilian soybean cultivars

Abstract

-The objective of this work was to determine genetic and environmental effects on beta-conglycinin and glycinin content in Brazilian soybean cultivars. The concentrations of these protein fractions were analyzed by scanning densitometry after electrophoresis, in 90 Brazilian soybean cultivars sown in Ponta Grossa, PR, in 2001. The effects of the sowing location were determined in the cultivar MG/BR 46 (Conquista), sown in 16 locations of Goiás and Minas Gerais states (Central Brazil), and in the cultivar IAS 5, sown in 12 locations of Paraná and São Paulo states (Southern Brazil), in 2002 soybean season. A significant variability for beta-conglycinin (7S) and glycinin (11S) protein fractions ratio was observed among the 90 Brazilian soybean cultivars. 'MS/BRS 169' (Bacuri) and 'BR-8' (Pelotas) presented the highest and the lowest 11S/7S ratios (2.76 and 1.17, respectively). Beta-conglycinin protein fractions presented more variability than glycinin protein fractions. Grouping test classified 7S proteins in seven groups, 11S proteins in four groups, and protein fraction ratios (11S/7S) in nine groups. Significant effect of sowing locations was also observed on protein fractions contents. There is a good possibility of breeding for individual protein fractions, and their subunits, without affecting protein content.Index terms: eletrophoresis, nutraceutic properties, protein fractions, seed, sowing location. Variação genética e ambiental e teores de beta-conglicinina e glicinina em cultivares de soja brasileirasResumo -O objetivo deste trabalho foi avaliar os efeitos da variação genética e ambiental sobre os teores de beta-conglicinina e glicinina em cultivares de soja brasileiras. A concentração dessas frações protéicas foi determinada por densitometria após eletroforese, em 90 cultivares de soja, semeadas em Ponta Grossa, PR, em 2001. Os efeitos dos locais de semeadura foram determinados na cultivar MG/BR 46 (Conquista), semeada em 16 locais de Goiás e Minas Gerais, e na cultivar IAS 5, semeada em 12 locais no Paraná e em São Paulo, em 2002. Foi observada variabilidade significativa quanto à razão entre as frações protéicas da beta-conglicinina (7S) e da glicinina (11S), entre as 90 cultivares avaliadas, em que a 'MG/BR 169' (Bacuri) apresentou a maior razão 11S/7S (2,76) e a 'BR-8' (Pelotas) a menor (1,17). As frações protéicas de beta-conglicinina apresentaram maior varibilidade do que as de glicinina. A análise de agrupamento discriminou as proteínas 7S em sete grupos, as 11S em quatro, e as razões 11S/7S em nove. Efeito significativo dos locais de semeadura também foi observado sobre os teores das frações protéicas. Existe uma boa possibilidade de melhoramento para as frações individuais de proteínas e suas subunidades, sem que o teor de proteína seja alterado.Termos para indexação: eletroforese, propriedades nutracêuticas, frações protéicas, semente, local de semeadura.

Physical characteristics and nutritional composition of some new soybean (Glycine max (L.) Merrill) genotypes

“…On the basis of this, high or low 11S or 7S genotypes/lines can be selected. 'SL 794' has 11S/7S ratio of 2.4 which is comparable to the earlier observations of Kitamura and Kaizuma (1981) and Carrao-Panizzi et al (2008). Content of β-conglycinin fractions, α′, α and β in 'SL 794' were respectively 7.6, 0.97 and 2.9% and glycinin acidic and basic subfractions were respectively 12 and 15.6% (Table 4).…”

Variation in genetic and environmental effects of beta-conglycinin (7S) and glycinin (11S) protein fractions in conventional and GM soybean cultivars grown in Southern Brazil

“…Showing that variations on climate or location had affect these protein fractions. Carrão Panizzi et al (2008), investigated the genetic variation and environment effects for different Brazilian cultivars and found higher values than those observed in our study, which confirms variability due to different cultivars, soybean season and locations. They also worked with a larger number of soybean genotypes and observed a range of 2.76 for cultivar BRS 169-Bacuri to 1.18 ratio of 11S/7S for cultivar BR-8 Pelotas.…”

“…The amount of protein corresponds to 40% and oil 20% (PIPOLO, 2002). About 70% of the protein corresponding to glycinin (11S) and β-conglycinin (7S) (CARRÃO-PANIZZI, 2008). The 7S globunin, is a trimeric glycoprotein consisting of three types of subunits (α, α' and β) and the 11S fraction has subunits A1aB2, A1bB1b, A2B1a, A3B4 and A5A4B3 (NIELSEN et al, 1995).…”

“…Studies on characterization and functional properties of the proteins glycinin and β-conglycinin in different soybean cultivars were carried out (MOLINA; PAPADOULOS; LEDWARD, 2001, RIBLETT et al, 2001, KHATIB et al, 2002, CARRÃO-PANIZZI et al, 2008. Both fractions are also capable of stimulating activity receptor for low density cholesterol (LDL), (ANTHONY, 2000;ERDMAN, 2000).…”

QTL mapping for protein content in soybean cultivated in two tropical environments

“…bras., Brasília, v.43, n.11, p.1533Brasília, v.43, n.11, p. -1541Brasília, v.43, n.11, p. , nov. 2008 high nutritional value for human and animal consumption (Wilson, 2004). Soybean breeding programs have, therefore, emphasized the development of varieties with high protein content, given the economic importance of this trait (Carrão-Panizzi et al, 2008).…”