Esterase profile in the postembryonic development of Rhipicephalusmicroplus

Abstract

-The objective of this work was to analyze the pattern of esterase activity in the development stages of Rhipicephalus microplus by nondenaturing polyacrylamide gel electrophoresis using specific staining for esterase. The electrophoretical results revealed the presence of nine regions displaying esterase activity, stained with both alpha-naphthyl acetate and beta-naphthyl acetate, and classified as alpha-beta-esterase. Stage-specific esterases were found, with the first nymphal and larval stages showing the greatest esterase activity throughout the development. An esterase called EST-4 was detected only in males and was considered sex-specific. There are differences in the esterase profile among the different postembryonic development stages of R. microplus.Index terms: cattle tick, resistance to acaricides, tick control. Perfil esterásico durante o desenvolvimento pós-embrionário de Rhipicephalus microplusResumo -O objetivo deste trabalho foi analisar o padrão de atividade da esterase nos estágios de desenvolvimento de Rhipicephalus microplus por meio de eletroforese em gel de poliacrilamida não desnaturante, com uso de coloração específica para esterase. Observou-se a presença de nove regiões com atividade esterásica, coradas tanto pelo alfa-naftil acetato como pelo beta-naftil acetato, e definidas como alfa-beta-esterases. Foram detectadas esterases estágio-específicas, e os estágios de ninfa de primeiro estádio e de larva foram os que mostraram maior atividade esterásica durante todo o desenvolvimento. A esterase EST-4 foi detectada apenas em machos e considerada sexo-específica. Existem diferenças quanto ao perfil esterásico nos diferentes estágios de desenvolvimento pós-embrionário de R. microplus.Termos para indexação: carrapato bovino, resistência a acaricidas, controle de carrapatos.

Sexual alteration in antioxidant response and esterase profile in Hyalomma anatolicum anatolicum (Acari: Ixodidae) ticks

“…Whole tick extracts were used to get the maximum number of esterases. Though only three major bands were detected in whole tick extracts in this study rather many other bands detected in different stages of life cycle in different species of ticks by many other studies (Jamroz et al 2000;Villarino et al 2003;Baffi et al 2005Baffi et al , 2007 Al-Shammery and Fetoh 2011) but it clearly indicates about the sexual polymorphism in esterase expression in H. a. anatolicum ticks. Moreover, esterase activity is directly linked with acaricide resistance (Abbas et al 2014).…”

Esterase mediated resistance in deltamethrin resistant reference tick colony of Rhipicephalus (Boophilus) microplus

“…Similar profiles in resistant larval homogenate (IVRI-IV) were observed in the present study when stained with both anaphthyl and b-naphthyl acetate. Baffi et al (2007) reported four esterase bands in larvae without any correlation with resistance. Similarly, Miller et al (2007) could not correlate permethrin resistance with metabolic detoxification via synergistic as well as TPP based inhibitors study.…”

MOLECULAR CHARACTERIZATION OF TWO CARBOXYLESTERASE GENES OF THE CITRUS RED MITE, Panonychus citri (ACARI: TETRANYCHIDAE)

“…It has been shown that four CarE genes were expressed at >two‐fold higher levels in pupae than adults of Anopheles gambiae (Strode et al, ). The different expression profile of PCE2 between male and female mites has been detected, while the study in R. microplus has also found that an EST called EST‐4 was sex‐specific (Baffi et al, ).…”