Atividade in vitro do extrato de própolis contra agentes bacterianos da mastite bovina

Loguercio, Andrea Pinto; Groff, Ana Cláudia Mello; Pedrozzo, Andréia Folleto; Witt, Niura Mazzini; Silva, Mariana Sá e; Vargas, Agueda Castagna de

doi:10.1590/s0100-204x2006000200021

Cited by 12 publications

(7 citation statements)

References 8 publications

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“…The results showed that both EPA and EPB showed antimicrobial activity against isolates of Staphylococcus spp., in agreement with previous studies indicated that the antibacterial activity of propolis is more pronounced against Gram-positive bacteria (Langoni et al 1996, Sforcin et al 2000, Pinto et al 2001, Fernandes Júnior et al 2003, Auricchio et al 2006, Trusheva et al 2010, Aguiar et al 2014. The antibacterial activity of the EPA, with 90.9% susceptibility, was similar to the Loguercio et al (2006) and Coelho et al (2010) which showed susceptibility of 94.4% in coagulase-positive Staphylococcus. When comparing the two extracts, it was verified that isolates of Staphylococcus spp.…”

Section: Discussionmentioning

confidence: 58%

“…In this sense, propolis, a resinous product produced by honey bees, is being used in the treatment of human and animal diseases (Coelho et al 2010) and has been shown to be a viable and quite promising alternative in the treatment of infections caused by S. aureus (Pinto et al 2001, Endler et al 2003, Auricchio et al 2006, Zeighampour et al 2014, Shahbaz et al 2015, Chen et al 2018) due to its proven antibacterial activity (Barbosa et al 2009, Araujo & Marcucci 2011. However the results using propolis are still conflicting (Pinto et al 2001, Loguercio et al 2006), due to its distinct and complex chemical constitution, necessitating the development of further research (Lustosa et al 2008, Rufatto et al 2017). In addition, there are few conclusive results regarding their action against MRSA, that play an important role in nosocomial infections (Taubes 2008) and a serious problem on a global scale (Astani et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Chemical composition and antimicrobial activity of two extract of propolis against isolates of Staphylococcus spp. and multiresistant bacterials

et al. 2019

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Amarante J.F., Ribeiro M.F., Costa M.M., Menezes F.G., Silva T.M.S., Amarante T.A.B., Gradela A. & Moura L.M.D. 2019. Chemical composition and antimicrobial activity of two extract of propolis against isolates of Staphylococcus spp. and multiresistant bacterials. Pesquisa Veterinária Brasileira 39 (9):734-743. There is a growing need to discover and develop alternative therapies for the treatment of mastitis caused by Staphylococcus spp. and multidrug-resistant bacterial infections. This study examined the chemical composition and antimicrobial potential of two propolis extracts (EPA and EPB) against seventy-seven isolates of Staphylococcus spp. obtained from subclinical bovine mastitis; three clinical strains of MRSA and two from clinical strains of S. aureus ATCC, identified as S. aureus ATCC 6538 and S. aureus ATCC 25923. The total phenolic content was determined by the Folin-Ciocalteau method, the total flavonoid content by the Dowd method and the phenolic profile was quantified by HPLC-DAD. The MBC values of the extracts were evaluated by broth microdilution method. The amount of total phenolic and flavonoid compounds was higher in EPA than EPB. Both extracts revealed the presence of caffeic, coumaric, cinnamic, ferulic and 3,4-dihydroxybenzoic acids, with higher concentrations of coumaric and cinnamic acids. Staphylococcus spp. isolates were susceptible to EPA (90.9%), EPB (83.1%) and oxacillin (80.5%). The oxacillin susceptible isolates were also susceptible to EPA (70.1%) and EPB (80.6%), whereas those oxacillin-resistant strains were also susceptible to EPA (40.0%) and to EPB (26.7%). MBC ranged from 34.3 to 68.7μm/mL for EPA and from 68.7 to 137.5μg/mL for EPB. Both extracts inhibited significantly (100%) the clinical strains of MRSA, S. aureus ATCC 6538 and S. aureus ATCC 25923 at the concentration of 68.7μg/mL. It is concluded that both extracts of propolis, whose main constituents are coumaric and cinnamic acids, have high antimicrobial activity against the microorganisms studied, and EPA also against oxacillin-resistant strains. These findings reinforce its potential use for the treatment of bovine mastitis.

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Section: Discussionmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Chemical composition and antimicrobial activity of two extract of propolis against isolates of Staphylococcus spp. and multiresistant bacterials

et al. 2019

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“…Estudos demonstram que a alta prevalência da mastite pode estar associada às más condições de higiene do ordenhador, bem como dos tetos e úberes das vacas antes, durante e após a ordenha (Oliveira et al, 2009 As cepas de S. aureus resistentes a diferentes grupos de antibióticos usados no combate da mastite bovina, muitas vezes estão relacionadas à má utilização de determinado produto, como, por exemplo, aplicação em subdosagens, período insuficiente de tratamento dos animais (Araújo, 1998;Fontana et al, 2010 (Loguercio et al, 2006).…”

Section: Resultsunclassified

Bovine mastitis caused by Staphylococcus aureus: susceptibility to antimicrobial drugs and the alcoholic extract of propolis

Saeki

Peixoto

Matsumoto

et al. 2012

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RESUMO -A mastite é um dos fatores que mais gera prejuízos à produção e industrialização do leite. O objetivo deste trabalho foi isolar a espécie Staphylococcus aureus das amostras de leite provenientes de 63 animais portadores de mastite, e verificar o seu perfil de sensibilidade antimicrobiana aos antibióticos comerciais e ao extrato de própolis à 30%. Dos 63 animais avaliados, 38 (60,32%) apresentaram crescimento de Staphylococcus aureus. Quanto ao teste de sensibilidade antimicrobiana, os antibióticos mais eficientes foram gentamicina (10 g), cefalexina (30 g) e ciprofloxacina (10 µg) com 100% de eficácia, seguida de norfloxacina (94,6%). Houve 35 (92,10%) animais com sensibilidade ao extrato alcoólico de própolis a 30%, com diâmetros de inibição entre 6 e 18 mm frente a volumes de 40L e 60 L. A investigação da sensibilidade antimicrobiana in vitro para cepas de Staphylococcus aureus isoladas de mastite bovina é de grande importância antes da indicação do seu tratamento terapêutico. O uso do extrato alcoólico de própolis a 30% na terapia antimicrobiana pode ser uma alternativa, pois seus componentes conferem-lhe grande valor na medicina natural popular e preventiva.Palavras-Chave: Antibiótico, mastite clínica, mastite subclínica, resistência.ABSTRACT -Mastitis is one of the factors that cause damage to production and processing milk. The aim of this study was to isolate the species Staphylococcus aureus from milk samples from 63 animals with mastitis, and check your profile antimicrobial sensitivity to antibiotics and the extract of propolis at 30%. Of 63 animals evaluated, 38 (60.32%) showed growth of Staphylococcus aureus. As for testing of antimicrobial sensitivity test, the most efficient antibiotics were gentamicin (10 g), cephalexin (30 g) and ciprofloxacin (10 g) with 100% efficiency, followed by norfloxacin (94.6%). There were 35 (92.10%) animals with sensitivity to the extract of propolis 30% with inhibition diameters between 6 and 18 mm in 40 L and 60 L. The investigation of in vitro antimicrobial susceptibility to Staphylococcus aureus isolated from bovine mastitis is extremely valuable to their treatment. The use of extract of propolis 30% in the antimicrobial therapy may be an alternative, because its components gives it excellent value in natural medicine and preventive.

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“…Variações são descritas no poder antimicrobiano da própolis frente a bactérias Gram positivas e Gram negativas (vargas et al, 2004;loguErCio et al, 2006 (dE vECChi;drago, 2007). Esses resultados divergentes podem estar relacionados à diferença da flora e clima entre Brasil (tropical ou subtropical) e Egito (temperado) (orsi et al, 2007;kujumgiEv et al, 1999).…”

Section: Discussionunclassified

Atividade antimicrobiana in vitro de extratos etanólicos de própolis de três estados brasileiros sobre Aeromonas hydrophila isoladas de peixes

et al. 2012

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RESUMOO presente estudo teve como objetivo avaliar a sensibilidade in vitro de Aeromonas hydrophila frente a extratos etanólicos de própolis (uma verde e duas marrons) obtidos em três estados brasileiros (Minas Gerais, Ceará e Pernambuco). Para verificar a atividade antimicrobiana in vitro da própolis, 15 isolados de A. hydrophila foram testados para determinar a Concentração Bactericida Mínima (CBM) dos extratos. Curvas de sobrevivência para o crescimento bacteriano foram determinadas pela incubação dos isolados em extratos etanólicos de própolis a 15% por 24 horas. As médias da CBM dos extratos de própolis foram 1,68% para a própolis verde, 2,31% para a própolis marrom do Ceará e 3,75% para a própolis marrom de Pernambuco. A curva de sobrevivência dos isolados demonstrou uma inibição parcial com até três horas de incubação. Este resultado é compatível com o efeito bacteriostático da própolis, o que pode ser de interesse para a terapia em aquicultura, como alternativa às poucas drogas antimicrobianas disponíveis. PALAVRAS-CHAVE: Aquicultura, A. hydrophila, apicultura, resistência, própolis. ABSTRACT EVALUATION OF ETHANOLIC EXTRACTS OF PROPOLIS FROM THREE BRAZILIAN STATES FOR IN-VITRO ANTIMICROBIAL ACTIVITY ON AEROMONAS HYDROPHILAISOLATED FROM FISH. The present study was aimed to evaluate the in-vitro susceptibility of Aeromonas spp. to ethanolic extracts of propolis (one green and two brown) from three Brazilian states (Minas Gerais, Ceará and Pernambuco). In order to verify the antimicrobial activity of the propolis in vitro, 15 Aeromonas spp. isolates were tested to determine the minimal bactericidal concentration (MBC) of the extracts. Ethanol at 70% was used as a control. Survival curves for the bacterial growth were determined by incubation of the isolates in ethanolic extracts of propolis at 15% for 24 hours. The averages of the MBC of propolis extracts were 1.68% for the green propolis of Minas Gerais, 2.31% for the brown propolis of Ceará, and 3.75% for the brown propolis of Pernambuco. The survival curve of the isolates showed partial inhibition until three hours of incubation. This result is compatible with the bacteriostatic effect of propolis that may be useful to antibiotic therapy in aquaculture, as an alternative to few available antimicrobial drugs.

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Atividade in vitro do extrato de própolis contra agentes bacterianos da mastite bovina

Cited by 12 publications

References 8 publications

Chemical composition and antimicrobial activity of two extract of propolis against isolates of Staphylococcus spp. and multiresistant bacterials

Chemical composition and antimicrobial activity of two extract of propolis against isolates of Staphylococcus spp. and multiresistant bacterials

Bovine mastitis caused by Staphylococcus aureus: susceptibility to antimicrobial drugs and the alcoholic extract of propolis

Atividade antimicrobiana in vitro de extratos etanólicos de própolis de três estados brasileiros sobre Aeromonas hydrophila isoladas de peixes

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