Bovine mastitis caused by Staphylococcus aureus: susceptibility to antimicrobial drugs and the alcoholic extract of propolis

Abstract

RESUMO -A mastite é um dos fatores que mais gera prejuízos à produção e industrialização do leite. O objetivo deste trabalho foi isolar a espécie Staphylococcus aureus das amostras de leite provenientes de 63 animais portadores de mastite, e verificar o seu perfil de sensibilidade antimicrobiana aos antibióticos comerciais e ao extrato de própolis à 30%. Dos 63 animais avaliados, 38 (60,32%) apresentaram crescimento de Staphylococcus aureus. Quanto ao teste de sensibilidade antimicrobiana, os antibióticos mais eficientes foram gentamicina (10 g), cefalexina (30 g) e ciprofloxacina (10 µg) com 100% de eficácia, seguida de norfloxacina (94,6%). Houve 35 (92,10%) animais com sensibilidade ao extrato alcoólico de própolis a 30%, com diâmetros de inibição entre 6 e 18 mm frente a volumes de 40L e 60 L. A investigação da sensibilidade antimicrobiana in vitro para cepas de Staphylococcus aureus isoladas de mastite bovina é de grande importância antes da indicação do seu tratamento terapêutico. O uso do extrato alcoólico de própolis a 30% na terapia antimicrobiana pode ser uma alternativa, pois seus componentes conferem-lhe grande valor na medicina natural popular e preventiva.Palavras-Chave: Antibiótico, mastite clínica, mastite subclínica, resistência.ABSTRACT -Mastitis is one of the factors that cause damage to production and processing milk. The aim of this study was to isolate the species Staphylococcus aureus from milk samples from 63 animals with mastitis, and check your profile antimicrobial sensitivity to antibiotics and the extract of propolis at 30%. Of 63 animals evaluated, 38 (60.32%) showed growth of Staphylococcus aureus. As for testing of antimicrobial sensitivity test, the most efficient antibiotics were gentamicin (10 g), cephalexin (30 g) and ciprofloxacin (10 g) with 100% efficiency, followed by norfloxacin (94.6%). There were 35 (92.10%) animals with sensitivity to the extract of propolis 30% with inhibition diameters between 6 and 18 mm in 40 L and 60 L. The investigation of in vitro antimicrobial susceptibility to Staphylococcus aureus isolated from bovine mastitis is extremely valuable to their treatment. The use of extract of propolis 30% in the antimicrobial therapy may be an alternative, because its components gives it excellent value in natural medicine and preventive.

Rupture of the mammary vein in a Holstein cow with mastitis and udder edema: case report

“…It lives in the udder, upper and lower urogenital tract, and upper respiratory tract of animals, and also on the milker's hands; this bacterium was also isolated in the milk samples and in the serosanguinous fluid collected herein. This microorganism has been frequently identified in mammary quarters as causing mastitis in the immediate postpartum period, especially where correct hygiene practices are not carried out during milking and in highly contaminated environments (Radostits et al, 2007;Saeki et al, 2012;Bandeira et al, 2013;Castelani et al, 2013;Jamali et al, 2014;Acosta et al, 2016). S. aureus is among the most important Staphylococcus species that causes mastitis.…”

“…Bacteria belonging to the genus Staphylococcus are the predominant causes of mastitis in the main milk-producing species in Brazil, and Staphylococcus aureus has a higher prevalence than other species in milk samples from cases of bovine mastitis, both in Brazil and internationally (Saeki et al, 2012;Bandeira et al, 2013;Castelani et al, 2013;Jamali et al, 2014;Acosta et al, 2016). The disease can be hyperacute, although the subclinical and chronic subclinical forms may also present clinical episodes (Quinn et al, 2002;Nader Filho et al, 2007).…”

Effects of associations of tannins from Anacardium occidentale and Anadenanthera colubrina with cephalosporin against bovine Staphylococcus aureus isolates

“…Among the antibiotics most prescribed to treat infections caused by S. aureus, 1st generation cephalosporin (Cephalexin) presents action against many bacteria including: S. aureus, Staphylococcus epidermides, Streptococcu spneumoniae, Streptococcus pyogenes, Eschericha coli, Haemophilus influenzae, Proteus mirabilis, Klebsiella pneumoniae and Moraxella catarrhalis (PRESCOTT et al, 2002;BERTOLDI, 2010;SAEKI et al, 2011), and constitutes a first option for skin infections, in which Staphylococcus aureus is the most probable causative agent (WHITE, 1996;PRESCOTT et al, 2002). Considering the increasing prevalence of multidrug-resistant S. aureus, which causes many diseases in humans and in animals (FREITAS et al, 2005;RUEGG, 2007), the use of tannins, associated with conventional antibiotics, can reduce morbidities and combat disease.…”

Chemical composition and antimicrobial activity of two extract of propolis against isolates of Staphylococcus spp. and multiresistant bacterials

“…Approximately 140 different etiological agents may cause bovine mastitis, mainly in the subclinical form, of which the most prevalent contagious microorganisms have been Staphylococcus aureus, Streptococcus spp., Streptococcus agalactiae, Staphylococcus spp. and Corynebacterium bovis, in pure culture or in association (Costa 2001, Santos & Fonseca 2007, Ribeiro et al 2009, Martins et al 2010, Saeki et al 2012. S. aureus is recognized worldwide as a cause of several purulent diseases in humans and animals (Bean & Griffin 1990) and also an important cause of food poisoning in humans (Omoe et al 2002).…”