Macropropagation and micropropagation of Ziziphus spina-christi

Assareh, M H; Sardabi, H

doi:10.1590/s0100-204x2005000500006

Cited by 21 publications

(9 citation statements)

References 8 publications

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“…In our experiments, treatments with calcium hypochlorite [Ca(ClO) 2 ] were satisfactory for sterilization ( Figure 2). Assareh and Sardabi (2005) reported that among treatments with Ca(ClO) 2 , NaOCl and HgCl 2 for tissue culture explants sterilization of Ziziphus spina-christi (L.)Desf., Ca(ClO) 2 in concentration of 5% and duration of 20 minutes was the most efficient. Comparing the effect of HgCl 2 and NaOCl in our experiment, NaOCl was found to be better, with more survived explants (Figure 3).…”

Section: Resultsmentioning

confidence: 99%

In vitro sterilization procedures for micropropagation of ‘oblacinska’ sour cherry

et al. 2013

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Surface sterilization is the most important step in preparation of explants for micropropagation, because controlling fungal and bacterial contamination of woody plant from field sources is very difficult. Six sterilizing agents: sodium hypochlorite (NaOCl), calcium hypochlorite [Ca(ClO) 2 ], sodium dichloroisocyanurate (DICA), mercuric (II) chloride (HgCl 2), silver nitrate (AgNO 3) and hydrogen peroxide (H 2 O 2) were tested for sterilization of 'Oblačinska' sour cherry buds, by varying their concentration and time of exposure. The aim of this study was to establish best surface sterilization for in vitro propagation of 'Oblačinska' sour cherry. Aseptic cultures of 'Oblačinska' sour cherry were established from axillary buds which were placed in nutrient medium, supplemented with plants hormones 6-benzylaminopurine (BA), 1naphthaleneacetic acid (NAA) and gibberellic acid (GA 3). The results indicated that among these sterilizing agents silver nitrate (AgNO 3) at concentration of 1% for 20 minutes was the best for controlling the infection, whereas sterilization with sodium dichloroisocyanurate (DICA) at concentration of 1% for 10 minutes was not satisfactory.

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Section: Resultsmentioning

confidence: 99%

In vitro sterilization procedures for micropropagation of ‘oblacinska’ sour cherry

et al. 2013

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“…Since Ziziphus spina [christti] is a cross-pollinated plant, a wide range of genetic variability exists in the nature. Vegetative propagation, particularly in vitro propagation, assumes importance for mass and clonal propagation (Assareh and Sardabi 2005). Since seeds of Z. spina [christti] require scarification treatments to germinate (Moustafa et al 1998), vegetative propagation plays a significant role in its improvement in breeding programs.…”

Section: Introductionmentioning

confidence: 99%

Contamination control of microbe Ziziphus spina [christti] seed in vitro culture

Ahmadi

Nasr

Jalilvand

et al. 2012

Trees

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Ziziphus spina [christti] is a naturally distributed tree in subtropical, arid and semi-arid parts of Iran. It is ecologically and economically important due to its tolerance to drought and salinity. Most tree seeds are infected with parasitic and saprophytic microorganisms which decrease the seed germination and seedling establishment. The goal of this paper was to evaluate the ability of selected chemical solutions to inhibit the growth of variety of microbial contaminants in Z. spina Contaminants were identified according to their morphological and cultural characteristics. Bacterial contaminants included Xanthomonas sp. While Fungal isolates were Fusarium, Penicillium, Alternaria, Rhizopus, and Aspergillus. Our experiment reveals that 4% NaOCl followed by benomyl is the best sterilization treatment for Z. spina [christti] seeds, since the highest number of germination and highest number of sterilized seeds was observed after this treatment.

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“…Furthermore, the collecting time of buds and grafts might have negatively influenced the experiment results. Assareh and Sardabi (2005), for instance, obtained better results on the grafting of Ziziphus spina-christi (L.) Desf. performed in autumn than in spring.…”

Section: Graftingmentioning

confidence: 99%

“…Although there are studies that testify the importance of different aspects on the grafting success, such as the used method, rootstock and graft origin, rootstock age and environmental conditions (ASSAREH; SARDABI, 2005), the studied factors did not influence the evaluated variables. Furthermore, the collecting time of buds and grafts might have negatively influenced the experiment results.…”

Section: Graftingmentioning

confidence: 99%

Vegetative propagation of bauhinia x blakeana, an ornamental sterile tree

et al. 2013

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-Bauhinia x blakeana (B. purpurea x B. variegata) is a natural hybrid that has been cultivated in gardens, streets and parks. Due to its sterility, it must be vegetatively propagated. The objective of this work was to evaluate the viability of cuttings and grafting on its propagation. Semi-woody cuttings were collected during four seasons and treated with 0; 1,000; 2,000; and 3,000 mg L -1 of IBA. The experimental design was entirely randomized and the treatments were arranged in a 4x4 factorial scheme (four collecting times x four IBA concentrations) and five replications with 10 cuttings each, per collecting time and per IBA concentration. Characteristics of roots and shoots were evaluated after 90 days. The data means were compared by the Tukey test and submitted to the polynomial regression analysis. For the grafting experiment, B. variegata and B. variegata var. candida plants of six and 12 months were used as rootstocks and the splice graft and T-budding methods were tested. The experimental design was entirely randomized and the treatments were arranged in a 2x2x2 factorial scheme (two rootstock species x two grafting methods x two rootstock ages) and four replications with five plants each, per rootstock species, per grafting method and per rootstock age. Characteristics of shoots were evaluated after 90 days and the data means were compared by the Tukey test. B. x blakeana can be propagated by semi-woody cuttings collected in spring, without IBA application, or in summer, with the application of 3,000 mg L -1 of IBA. The tested grafting methods were not effective. de AIB. O delineamento foi inteiramente casualizado, e os tratamentos foram arranjados em esquema fatorial duplo (quatro estações do ano x quatro concentrações de AIB) e cinco repetições com 10 estacas cada, por concentração de AIB e por estação do ano. Características vegetais e radiculares das estacas foram avaliadas após 90 dias. As médias dos dados foram comparadas pelo teste de Tukey e submetidas à análise de regressão polinomial. Para a enxertia, mudas de seis e 12 meses de B. variegata e B. variegata var. candida foram usadas como porta-enxerto, e os métodos de garfagem inglês simples e borbulhia "T" invertido foram testados. O delineamento foi inteiramente casualizado, e os tratamentos foram arranjados em esquema fatorial triplo (duas espécies de porta-enxerto x dois tipos de enxertia x duas idades do porta-enxerto) e quatro repetições

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Macropropagation and micropropagation of Ziziphus spina-christi

Cited by 21 publications

References 8 publications

In vitro sterilization procedures for micropropagation of ‘oblacinska’ sour cherry

In vitro sterilization procedures for micropropagation of ‘oblacinska’ sour cherry

Contamination control of microbe Ziziphus spina [christti] seed in vitro culture

Vegetative propagation of bauhinia x blakeana, an ornamental sterile tree

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