Genetic divergence among tomato leafminer populations based on AFLP analysis

Abstract

-The objective of this work was to determine the genetic differences among eight Brazilian populations of the tomato leafminer Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), from the states of Espírito Santo (Santa Tereza), Goiás (Goianápolis), Minas Gerais (Uberlândia and Viçosa), Pernambuco (Camocim de São Félix), Rio de Janeiro (São João da Barra) and São Paulo (Paulínia and Sumaré), using the amplified fragment length polymorphism (AFLP) technique. Fifteen combinations of EcoRI and MseI primers were used to assess divergence among populations. The data were analyzed using unweighted pair-group method, based on arithmetic averages (UPGMA) bootstrap analysis and principal coordinate analysis. Using a multilocus approach, these populations were divided in two groups, based on genetic fingerprints. Populations from Goianápolis, Santa Tereza, and Viçosa formed one group. Populations from Camocim de São Félix, Paulínia, São João da Barra, Sumaré, and Uberlândia fitted in the second group. These results were congruent with differences in susceptibility of this insect to insecticides, previously identified by other authors.

Association of nymphs and adults of Ephemeroptera (Insecta) using the amplified fragment length polymorphism (AFLP) technique

“…The AFLP technique has been used to establish differences among species or populations based on molecular polymorphisms (Najimi et al, 2002;Zhong et al, 2002;Benavides et al, 2003;Suinaga et al, 2004). Furthermore, we associated nymphs and adults of the same species by getting molecular similarities among individuals.…”

Molecular characterisation of tomato leaf miner Tuta absoluta populations obtained from different geographical locations of India

“…Based on the ITS 1, 2 and COI sequences of T. absoluta populations from the Mediterranean Basin and South America showed high genetic homogeneity (Cifuentes et al, 2011). While, eight Brazilian populations of T. absoluta using the Amplified Fragment Length Polymorphism (AFLP) technique showed differences in the populations responses to insecticides as well as host plants (Suinaga et al, 2004). Other studies on Tunisian T. absoluta using Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) technology resulted high genetic diversity as well as in a significant differentiation between populations (Bettaïbi et al, 2012).…”

“…Molecular characterization and DNA barcoding is a standard taxonomic method that uses a short genetic marker in an insect DNA to identify a species (Jalali et al, 2015). Partial DNA sequences of the mitochondrial gene such as cytochrome c oxidase subunit I (COI), Internal Transcribed Spacer (ITS) regions of rDNA and other molecular markers have been used to assess genetic diversity of T. absoluta populations (Suinaga et al, 2004;Cifuentes et al, 2011;Bettaïbi et al, 2012;2016;Shashank et al, 2018). The study of genetic variability of invasive T. absoluta is essential to develop an efficient Integrated Pest Management (IPM) programs (Bettaïbi et al, 2012).…”

The tomato borer Tuta absoluta in South America: pest status, management and insecticide resistance

“…Local selection favoured by weather conditions and dispersal seems important for resistance evolution, and should be considered in designing pest management programmes (Siqueira et al ., ; Silva et al ., ). In fact, variation for further selection for insecticide resistance does exist in Brazilian populations of T. absoluta , leading to a high risk of insecticide resistance evolution (Suinaga et al ., ; Silva et al ., ).…”