Protocolo para produção de material propagativo de batata em meio líquido

Pereira, Jonny Everson Scherwinski; Fortes, Gérson Renan de Luces

doi:10.1590/s0100-204x2003000900003

Cited by 29 publications

(25 citation statements)

References 16 publications

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“…As a result, new approaches have been developed to enhance the outcome and reduce the costs of micropropagation technique (PEREIRA et al, 1999;FORTES, 2003;PINHO, 2003).…”

Section: Introductionmentioning

confidence: 99%

Growth regulators and substrates for Oncidium baueri Lindl. micropropagation

Rodrigues

Nadal

Camargo

et al. 2016

SCA

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An adequate concentration of growth regulators as well as the replacement of agar by an alternative medium may be promising from practical and financial points of view to produce orchid plants by micropropagation. The objective of this work was to evaluate different concentrations of growth regulator and alternative substrates for agar replacement in culture medium for in vitro multiplication and rooting of Oncidium baueri. In the explant multiplication phase, two experimental factors were evaluated-various concentrations of 6-benzylaminopurine (BAP) (0.0, 1.0, 2.0, and 3.0 mg L -1 ) and substrates (agar, vermiculite, and coconut fiber) added to MS medium. In the rooting phase, different concentrations of indole butyric acid (IBA) (0.0, 0.5, 1.0, and 1.5 mg L -1 ) were added to culture medium containing the same substrate. Six months after the experiments were initiated, the survival percentage, number of leaves, shoots, and roots and length of the aerial part and the major root were evaluated. The results suggested that addition of 1.0 mg L -1 BAP is necessary for the O. baueri in vitro multiplication phase, but IBA is not necessary in the rooting phase. For the substrate, vermiculite is not indicated as an agar replacement. In contrast, coconut fiber can be used in both multiplication and rooting phases of Oncidium baueri in vitro culture. Key words: Orchid. Coconut fiber. Culture medium. Vermiculite. ResumoNa micropropagação de orquídeas, a concentração adequada de reguladores de crescimento, bem como a substituição do ágar por substratos alternativos no meio de cultura podem ser promissoras do ponto de vista da praticidade e da economia financeira para a obtenção de mudas. Assim, o objetivo do trabalho foi avaliar diferentes concentrações de reguladores de crescimento e substratos alternativos em substituição ao ágar no meio de cultura para a multiplicação e o enraizamento in vitro de Oncidium baueri. Na fase de multiplicação dos explantes, foram avaliados dois fatores: concentração de benzilaminopurina (BAP) (0,0; 1,0; 2,0 e 3,0 mg L -1 ) e substrato (ágar, vermiculita e fibra de coco) no meio de cultura MS e, no período de enraizamento, foram testadas concentrações de ácido indolbutírico (AIB) (0,0; 0,5; 1,0

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“…As a result, new approaches have been developed to enhance the outcome and reduce the costs of micropropagation technique (PEREIRA et al, 1999;FORTES, 2003;PINHO, 2003).…”

Section: Introductionmentioning

confidence: 99%

Growth regulators and substrates for Oncidium baueri Lindl. micropropagation

Rodrigues

Nadal

Camargo

et al. 2016

SCA

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“…A alteração do estado físico do meio de cultura modifica a resistência física e de contato dos explantes com o meio, podendo influenciar no desenvolvimento de plântulas in vitro (Chen & Ziv, 2001). Assim, o maior contato dos explantes com meio, que acontece nos cultivos em meio líquido, pode aumentar a absorção de água e nutrientes quando comparado ao meio semi-sólido, favorecendo a taxa de assimilação de nutrientes, altura e multiplicação de brotos e, ainda, acúmulo de massa seca (Pereira & Fortes, 2003). O estado físico do meio de cultura, portanto, pode estar relacionado ao desempenho assimilatório das plantas durante o período de cultivo do material vegetal (Ziv, 1995).…”

Section: Introductionunclassified

Estado Físico Do Meio De Cultura Na Propagação in Vitro De Bromeliaceae

Mengarda¹,

Povoas²,

Debiasi³

et al. 2009

RSA

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RESUMOO objetivo deste estudo foi avaliar o efeito do estado físico do meio de cultura sobre o comportamento in vitro de Neoregelia cruenta, Tillandsia stricta, Vriesea gigantea, V. guttata e V. incurvata. Brotos micropropagados foram cultivados em meio de cultura MS, com 30 g de sacarose, 2,5 mg dm -3 BAP, e 0,5 mg dm -3 ANA estabelecendo os seguintes tratamentos: T1-semi-sólido com 7 g de ágar; T2-líquido estático; T3-líquido sob-agitação a 90 rpm; e T4-líquido estático com ponte de papel filtro. Após 30 dias de cultivo, a utilização do meio de cultura líquido estático apresentou melhores resultados em relação à taxa média proliferativa em todas as espécies (9,4 brotos explante -1 em N.cruenta, 5,6 em T. stricta, 11,5 em V. gigantea, 9,2 em V. guttata e 3,9 em V. incurvata). Em relação à altura média dos brotos, destacou-se para N. cruenta o meio líquido sob agitação (2,83 cm), para T. stricta e V. incurvata o semi-sólido (1,76 cm e 2,02 cm, respectivamente) e para V. gigantea e V. guttata o líquido estático (0,61 cm e 1,48 cm, respectivamente). A utilização de meio de cultura MS líquido estático mostrou-se como sendo o mais adequado para o cultivo in vitro das espécies de bromélias estudadas.Palavras-chave: micropropagação; conservação da biodiversidade; bromélias ornamentais. ABSTRACTThe objective of this study was to evaluate the effect of the physical state of the culture medium on the behavior in vitro of the bromeliaceas Neoregelia cruenta, Tillandsia stricta, Vriesea gigantea, V. guttata e V. incurvata. Micropropagated shoots had been cultivated in culture medium MS, with 30 g sucrose, 2,5 mg dm -3 BAP, and 0,5 mg dmANA, establishing the treatments: T1-half-solid with 7 g agar; T2-static liquid; T3-liquid under-agitation of 90 rpm; and T4-static liquid with bridge of paper filter. After 30 days, the use of static way of liquid culture presented better results in relation of proliferative average rate in all the species (9.4 shoots in N. cruenta, 5.6 in T. stricta, 11.5 in V. gigantea, 9.2 in V. guttata and 3.9 in V. incurvata). In relation the average height of the shoots, was distinguished for N. cruenta the liquid under-agitation (2.83 cm), T. stricta and V. incurvata the semisolid (1.76 cm and 2.02 cm, respectively) and V. gigantea and V. guttata the static liquid (0.61 cm and 1.48 cm, respectively). The use of medium MS static liquid was more suitable for in vitro culture of bromeliads species studied.

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“…Soil desinfestation generally is done with chemicals such as methyl bromide, which eliminates both pathogenic and beneficial microorganisms. This technique usually results in low yield of minitubers per plant, generally five or less, and is a serious constraint to the production (Daniels et al, 2000;Pereira, 2002;Fortes and Pereira, 2003). The development of more efficient and productive systems with lower environmental impact would improve the quality of the propagative material used by the potato growers, directly affect the crop yields and reduce the production costs (Pereira and Fortes, 2003;Pereira and Fortes, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…This technique usually results in low yield of minitubers per plant, generally five or less, and is a serious constraint to the production (Daniels et al, 2000;Pereira, 2002;Fortes and Pereira, 2003). The development of more efficient and productive systems with lower environmental impact would improve the quality of the propagative material used by the potato growers, directly affect the crop yields and reduce the production costs (Pereira and Fortes, 2003;Pereira and Fortes, 2004). The use of hydroponic systems offers an excellent alternative to reduce the potential risks of root and tuber contamination by the soil pathogens and also for eliminating the need of chemical disinfectants, generally very harmful to the human health and environment (Medeiros et al, 2001;Pereira and Fortes, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…The development of more efficient and productive systems with lower environmental impact would improve the quality of the propagative material used by the potato growers, directly affect the crop yields and reduce the production costs (Pereira and Fortes, 2003;Pereira and Fortes, 2004). The use of hydroponic systems offers an excellent alternative to reduce the potential risks of root and tuber contamination by the soil pathogens and also for eliminating the need of chemical disinfectants, generally very harmful to the human health and environment (Medeiros et al, 2001;Pereira and Fortes, 2003). The aim of this study was to evaluate the efficiency of hydroponic systems for pre-basic seed potato production, taking into account the origin of propagative material used (minitubers and plants from in vitro culture).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Production of pre-basic potato seed by polyvinyl chloride PVC: articulate gutters hydroponic system

Scherwinski‐Pereira

Medeiros

Fortes

et al. 2009

Braz. arch. biol. technol.

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The development of more efficient and productive systems for pre-basic seed potato production would improve the quality of the propagative material used by the potato growers, directly affecting the crop yields. A two-year experiment was carried out to evaluate the potato pre-basic seed production by two types of hydroponic systems (fibrocement tiles and articulated PVC gutters), two cultivars (`Baronesa` and `Eliza`) and two types of propagative material (plants coming from in vitro culture and minitubers). The PVC gutters system was highly efficient. When using minitubers, this system reached multiplication rates up to 74 tubers per plant. Minitubers were more productive than in vitro plants, independent of cultivar and hydroponic system utilized.

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Protocolo para produção de material propagativo de batata em meio líquido

Cited by 29 publications

References 16 publications

Growth regulators and substrates for Oncidium baueri Lindl. micropropagation

Growth regulators and substrates for Oncidium baueri Lindl. micropropagation

Estado Físico Do Meio De Cultura Na Propagação in Vitro De Bromeliaceae

Production of pre-basic potato seed by polyvinyl chloride PVC: articulate gutters hydroponic system

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