Total lipid and fatty acid accumulation during basidiospore formation in the ectomycorrhizal fungus Pisolithus sp.

Campos, André Narvaes da Rocha; Costa, Maurício Dutra; Tótola, Marcos Rogério; Borges, Arnaldo Chaer

doi:10.1590/s0100-06832008000400017

Cited by 16 publications

(22 citation statements)

References 21 publications

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“…Although these propagules are used for inoculation in nurseries, mycorrhization is often reported to show medium to low efficiency (Brundrett et al 2005). This fact has stimulated some studies on basidiospore formation aiming at a better understanding of the fungal biology and of the main factors that stimulate the germination process (Campos et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…The main stages of basidiosporogenesis in the ectomycorrhizal fungus P. microcarpus have been investigated, especially with respect to the constitution of lipid reserves (Campos et al 2008). P. microcarpus accumulates different fatty acids in its basidiocarp, with oleic acid being the main storage compound in the basidiospores (Campos et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…P. microcarpus accumulates different fatty acids in its basidiocarp, with oleic acid being the main storage compound in the basidiospores (Campos et al 2008). Lipids are important for basidiosporogenesis, as evidenced by the remarkable distribution and content of these compounds in lipid bodies throughout the entire development of the fungal basidiocarp (Campos et al 2008). However, besides the conspicuous presence of fatty acids in P. microcarpus sporocarps, the presence of other storage compounds in the hyphae, basidia, and basidiospores during basidiocarp development is still unknown.…”

Section: Introductionmentioning

confidence: 99%

“…Lipids, trehalose, and glycogen, stored in the vegetative and reproductive fungal structures, guarantee the provision of metabolic precursors and energy for the diverse array of metabolic and morphogenetic processes that take place during the fungal life cycle (Bago et al 2002;Campos et al 2008). The composition of the lipid and carbohydrate fractions of fungal reserves is not only important for basidiocarp development, but also for the subsequent germination of fungal spores.…”

Section: Introductionmentioning

confidence: 99%

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Histochemistry and storage of organic compounds during basidiosporogenesis in the ectomycorrhizal fungus Pisolithus microcarpus

Campos

Costa

2010

World J Microbiol Biotechnol

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Knowledge on the distribution and storage of different organic compounds during basidiosporogenesis in P. microcarpus is paramount to a better understanding of basidiospore recalcitrance to germination. The objective of this work was to detect the presence and distribution of phenolics, reducing sugars, starch, glycogen, total polysaccharides, RNA, and proteins during P. microcarpus basidiosporogenesis. Starch and reducing sugars were not detected in the fungal basidiocarps, while other polysaccharides predominated in the extracellular matrix at the base of the basidiocarp containing unconsolidated peridioles. Phenolics were also detected in this region. Glycogen was present inside the hyphae, basidia, and basidiospores and constitutes an important storage compound in the fungal basidiocarps. In mature basidiospores, RNA accumulation occurred at discrete locations in the cytoplasmatic periphery, while polysaccharides and proteins were shown to predominate in the cell wall. The presence of glycogen, RNA, and proteins inside the basidiospores strongly indicates provision for future germination and suggests that other factors may also influence basidiospore recalcitrance.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Histochemistry and storage of organic compounds during basidiosporogenesis in the ectomycorrhizal fungus Pisolithus microcarpus

Campos

Costa

2010

World J Microbiol Biotechnol

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“…Ainda, pode ser realizada a extração dos lipídios da biomassa, para posterior uso na produção de biodiesel, ou lipídios insaturados (CAMPOS et al, 2008;SILVA;FREITAS, 2008).…”

Section: Introductionunclassified

INDUÇÃO DA SÍNTESE DE LIPÍDIOS E PROTEÍNAS POR Aspergillus niger

Zen¹,

Silva²,

Reinehr³

et al. 2014

CIATEC

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ResumoA produção de lipídios e de proteínas em biomassas fúngicas pode apresentar aplicações, por exemplo, o seu uso para a produção de biocombustíveis como o biodiesel, para a extração de lipídios insaturados ou ainda pela incorporação da biomassa em rações animais. O crescimento fúngico pode ser realizado por meio de bioprocesso em estado sólido, consistindo um método de produção que permite a reutilização de resíduos agroindustriais, agregando valor a esses. Objetivou-se o cultivo do fungo Aspergillus niger em resíduos agroindustriais para o acúmulo de lipídios e proteínas intracelulares. O crescimento fúngico foi realizado em fermentação em estado sólido, utilizando farelo de trigo com umidade de 60% ao qual se adicionou indutores para a síntese de lipídios. Observou-se maior acúmulo de lipídios nos experimentos adicionados de sulfato ferroso ou glicose/ sulfato ferroso, obtendo-se respectivamente 4,2 e 3,8 vezes de aumento no teor de lipídios em relação ao tempo inicial de fermentação. O maior acúmulo de proteínas foi obtido no experimento que continha glicose, de 2,8 vezes em comparação com o experimento sem indutores. A indução da síntese de lipídios e proteínas pode ser afetada pelas condições do meio de cultivo, dependendo da aplicação almejada. Palavras-chave: Fermentação em estado sólido. Glicerol. Glicose. Indutores. Sulfato ferroso. AbstractThe production of lipids and proteins in fungal biomass may submit applications such as their use for the production of biofuels such as biodiesel, for the extraction of unsaturated lipids or by the incorporation of biomass into animal feed. Fungal growth can be performed by solid state fermentation, consisting of a production method that allows reuse of agroindustrial waste, adding value to it. We aimed the cultivation of the fungus Aspergillus niger in agroindustrial residual for intracellular accumulation of lipids and proteins. Fungal growth was carried out in solid state fermentation using wheat bran with 60% humidity and added to induce lipid synthesis. We observed higher lipids accumulation in the experiments with the addition of ferrous sulfate or glucose/ ferrous sulfate, yielding respectively 4.2 and 3.8 fold increase in lipid content in the initial fermentation time. The greater accumulation of protein was obtained in the experiment that contained glucose, of 2.8 times compared to the experiment without inducers. The induction of lipid synthesis and proteins can be affected by the conditions of the medium, depending on the desired application.

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