Presence of virulence factors in Enterococcus faecalis and Enterococcus faecium susceptible and resistant to vancomycin

Comerlato, Carolina Baldisserotto; Resende, Mariah Costa Carvalho de; Caierão, Juliana; d’Azevedo, Pedro Alves

doi:10.1590/s0074-02762013000500009

Cited by 99 publications

(81 citation statements)

References 29 publications

(42 reference statements)

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“…Even though the percentage of biofilm production from infecting isolates (54 %) is comparable to that reported in previous studies (38-93 %) (Comerlato et al, 2013;Duprè et al, 2003;Gozalan et al, 2015;Ira et al, 2013;Tsikrikonis et al, 2012), biofilm synthesis from colonizing isolates (13 %) was low (Johansson & Rasmussen, 2013;Tsikrikonis et al, 2012). Furthermore, Tsikrikonis et al (2012) found that 70 % of colonizing isolates were biofilm producers, whereas, in the study of Johansson & Rasmussen (2013), biofilm synthesis was more common in normal flora isolates as compared with those recovered from infective endocarditis.…”

Section: Discussionsupporting

confidence: 87%

“…They constitute an important cause of nosocomial infections, the most common species being Enterococcus faecalis and Enterococcus faecium (Abriouel et al, 2008;Comerlato et al, 2013;Papadimitriou-Olivgeris et al, 2014). Their ability to produce biofilm has been implicated in the induction of infections (Upadhyaya et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Their ability to produce biofilm has been implicated in the induction of infections (Upadhyaya et al, 2011). The Enterococcus surface protein (Esp), encoded by the esp gene, acts as an adhesin and is involved in the formation of biofilms (Upadhyaya et al, 2011), although this association has not been not confirmed by other investigators (Comerlato et al, 2013). Many proteins have been implicated in enterococcal virulence (Elsner et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Nowadays, a great proportion of nosocomial enterococcal infections are caused by vancomycin-resistant enterococci (VRE) strains (Comerlato et al, 2013). The most commonly identified genes conferring resistance to glycopeptides are vanA and vanB genes, whereas the majority of VRE are Enterococcus faecium (90 %) (PapadimitriouOlivgeris et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…A high proportion of enterococci from water samples often produce biofilm and carry virulence genes, which have been implicated in the induction of clinical infections. (Abriouel et al, 2008;Ahmad et al, 2014;Comerlato et al, 2013) The presence of such isolates in river or coastal waters poses an important public health problem since they can be a relevant source of resistance and virulence spread to humans.…”

Section: Introductionmentioning

confidence: 99%

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Biofilm synthesis and presence of virulence factors among enterococci isolated from patients and water samples

Papadimitriou-Olivgeris

Filippidou

Δρούγκα

et al. 2015

Journal of Medical Microbiology

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The goal of this study was to compare biofilm synthesis among enterococci recovered from clinical samples (infection or colonization) of patients as well as environmental samples in order to determine possible virulence factors and clonal relationship. During a two-year period, clinical samples (blood, catheter tips, bronchial secretions, wounds, peritoneal fluid, urine) and rectal swabs collected from hospitalized patients as well as environmental water samples were tested for the presence of Enterococcus faecalis and Enterococcus faecium. Antibiotic susceptibility testing was performed by the disc diffusion method and Etest. Strains were tested for the presence of vanA, vanB, esp, ace and asp genes by PCR. Clones were identified by PFGE (Sma I). From infected patients, 48 strains were identified: 24 Enterococcus faecium (10 vanApositive, 14 vancomycin-susceptible) and 24 Enterococcus faecalis (one vanA-positive, 23 vancomycin-susceptible). Among 143 colonizing isolates, 134 were Enterococcus faecium (58 vanA-positive, 11 vanB-positive, 65 vancomycin-susceptible) and nine Enterococcus faecalis (three vanA-positive, two vanB-positive, four vancomycin-susceptible). Among 167 environmental water samples, 51 Enterococcus faecalis and 19 Enterococcus faecium isolates, all glycopeptide-susceptible, were recovered. In total, 64 strains produced biofilm, whereas 34 were esp-positive, 64 asp-positive and 54 ace-positive. Biofilm production was associated with the presence of esp (P,0.001) and ace genes (P50.021), being higher in infecting (P,0.001) and water (P 0.005) isolates as compared with colonizing ones. Clones of environmental waterstrains were different than the patients' clones. The differences found in the incidence of antibiotic resistance, virulence factors and clones suggest that hospital and water enterococci are of different origin.

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