A potentially probiotic strain of Enterococcus faecalis from human milk that is avirulent, antibiotic sensitive, and nonbreaching of the gut barrier

Anjum, Jasia; Zaidi, Arsalan; Barrett, Kim E.; Tariq, Muhammad

doi:10.1007/s00203-022-02754-8

Cited by 7 publications

(7 citation statements)

References 108 publications

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“…The CRISPR-Cas system has a defense mechanism against genetic viral contamination [54], which could also be mediated by restriction enzyme-encoding genes due to the absence of plasmids [51]. Otherwise, the absence of plasmids may be due to copy number, growing temperature or even isolation methods [50]. The presence of prophages in our strain was predicted.…”

Section: Discussionmentioning

confidence: 90%

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Genomic insights from Enterococcus faecium SMAN_Baghdad isolated from the honey bee gut and analysis of its potential antimicrobial activity against multidrug-resistant Pseudomonas aeruginosa

Al-Shimmary,

Al-Thwani

2024

Preprint

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Background Bacterial genetics and molecular biology have been investigated rapidly, accurately and revolutionary by high throughput whole genome sequencing. Enterococcus faecium has recently been widely used in fermentation and as a probiotic in food products to promote health, and its products have been used as alternative compounds to conventional antibiotics for bacterial eradication. Genomic analysis provides rapid information about its diversity and evolution, suggesting a significant molecular basis. Objective This study aimed to perform a genome analysis of Enterococcus faecium isolated from the honey bee gut and test the antagonistic activity of its bacteriocin like inhibitor substances against clinically MDR Pseudomonas aeruginosa. Methods The isolated bacterium SMAN_Baghdad was identified as Enterococcus faecium using 16S rRNA gene sequencing. The whole genome of E. faecium SMAN_Baghdad was sequenced and analyzed by bioinformatics tools. Results Our strain genome sequence has 40 contigs, and its genome size is 2,835,588 bp, with a guanine-cytosine(GC) percentage of 38.1%. It comprises 2,667 genes, including 2,599 coding sequences (CDSs) and 68 predicted RNAs (including 60tRNAs and others). Many genes encoding genes involved in the production of probiotics and metabolic properties, such as bacteriocin-like inhibitory substances (e.g., enterolycinA, enterocin SE-K4 and entarocin L50a and b),were detected in BLIS. BLISs exhibit significant antibacterial activity against MDR Pseudomonas aeruginosa Conclusion Analysis of genome supported the ability to consider bacteriocin-like inhibitory substances as bactericidal agents instead of common antibiotics.

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Section: Discussionmentioning

confidence: 90%

“…Adhesion is important for probiotics and commensal enterococci to hang on, promote colonization and prevent host elimination. Moreover, adhesion genes have been identi ed among enterococci identi ed as starter probiotic strains [50]. Other genes play critical roles in frightening competitive organisms.…”

Section: Discussionmentioning

confidence: 99%

Genomic insights from Enterococcus faecium SMAN_Baghdad isolated from the honey bee gut and analysis of its potential antimicrobial activity against multidrug-resistant Pseudomonas aeruginosa

Al-Shimmary,

Al-Thwani

2024

Preprint

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“…Additionally, the virulence factors genes were detected through E. faecium NT04 via using a genomic sequencing database, like genes encoded biofilm formation ( ebpAfm, ebpBfm, ebpCfm, srtC, bopD ), cell wall adhesion proteins ( acm, scm ) [ 26 ], and enzymes related to capsular polysaccharide biosynthesis and antiphagocytosis ( cps A, cps B). The presence of specific virulence factors does not mean that the harboring organism may be harmful [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…It is well known both that adhesion and capsule formation are essential even for commensal and probiotic enterococci to persist, promote colonization, and avoid elimination from the host. Moreover, genes involved in adhesion and capsule formation have also been identified among enterococci designated as starter/probiotic strains [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens

Tarek

Azmy

Khairalla

et al. 2023

Heliyon

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“…35 Not always pathogenic, Enterococcus faecalis can also be helpful and might be considered a potential probiotic, as shown in a study of E. faecalis in human milk. 36 If it is very challenging to eliminate E. faecalis perfectly, even by using any recommended irrigating Table 1. Oxygen and its relation to the root canal.…”

Section: Obturationmentioning

confidence: 99%

Oxygen as obturation biomaterial in endodontic treatment: development of novel membranous dental restoration system

Wahyudi¹,

Kusumasari

2023

F1000Res

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Complexities in obturation and difficulties in disinfection are the major problems that make endodontic treatment very time-consuming. A new perspective is needed to reduce the working time as well as to answer these two problems. Until now, none of the established obturation techniques for root canal filling can guarantee a perfect seal. Solid substances cannot be manipulated easily to fill the tiny branches of the root canal system hermetically. At the same time, anaerobes and facultative anaerobes, especially Enterococcus faecalis, are very dominant in endodontic infections discussion. As shown in many studies, it is extremely difficult to perfectly disinfect Enterococcus faecalis even by using some irrigating solutions with strong antibacterial properties. Under anaerobic conditions, the invasion efficiency of facultative anaerobes is increased. In case irrigation and disinfection cannot totally eliminate anaerobes and facultative anaerobes, a new strategy is also needed to deal with the bacteria that still survive in the root canal. Oxygen can fill the root canal system with ease, eliminate anaerobes, and prevent facultative anaerobes from being pathogenic. Therefore, using oxygen as obturation biomaterial after proper cleaning and shaping procedures is expected to solve the two major endodontic problems. The aim of this article was to discuss a new possible concept of dental restoration system that uses an oxygen-permeable membrane to decrease the time required in endodontic treatment. The membrane is placed at the orifice of a duct created to connect the entire root canal system with free air outside the restoration. The function of the membrane is more or less similar to the mask used during the COVID-19 pandemic, as it enables the oxygen to circulate while preventing any fluid, debris, and microorganisms from passing. We hypothesize that the oxygen circulating in the root canal system will also act as an antimicrobial agent that is constantly renewed.

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A potentially probiotic strain of Enterococcus faecalis from human milk that is avirulent, antibiotic sensitive, and nonbreaching of the gut barrier

Cited by 7 publications

References 108 publications

Genomic insights from Enterococcus faecium SMAN_Baghdad isolated from the honey bee gut and analysis of its potential antimicrobial activity against multidrug-resistant Pseudomonas aeruginosa

Genomic insights from Enterococcus faecium SMAN_Baghdad isolated from the honey bee gut and analysis of its potential antimicrobial activity against multidrug-resistant Pseudomonas aeruginosa

Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens

Oxygen as obturation biomaterial in endodontic treatment: development of novel membranous dental restoration system

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