Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens

Tarek, Nashwa; Azmy, Ahmed F.; Khairalla, Ahmed S.; Abdel-Fattah, Maha M.; Jefri, Ohoud A.; Shaban, Mohamed; Elsayed, Abdelrahman H.; El-Gendy, Ahmed O.

doi:10.1016/j.heliyon.2023.e16253

Cited by 2 publications

(1 citation statement)

References 53 publications

(57 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Staphylococcus is commonly isolated as a contaminant in nosocomial and environmental samples [35] [36] [37]. However, its biofilms are a contribution to antimicrobial resistance due to the acquisition of virulence genes and their existence in pathogenicity islands [38] [39] [40].…”

Section: Identification Of Biofilm-producing Isolatesmentioning

confidence: 99%

Antimicrobial Activities of Extracts of <i>Macrosphyra longistyla</i> against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps

Nwankwo,

Folarin,

Osho

2024

AiM

View full text Add to dashboard Cite

The world will benefit from more effective antimicrobial agents against oral conditions arising from the actions of biofilm forming bacteria. Also, information is lacking on the oral biofilm-forming bacterial diversity in Southwestern Nigeria. In this study, we isolate and characterize oral biofilm producing bacteria in the oral cavities of schoolchildren in Southwestern Nigeria.We also investigate the antimicrobial properties of Macrosphyra longistyla extracts against the biofilm-formers and the toxicity of potent extracts. Samples were obtained from 109 schoolchildren aged 4 -14 years from Lagos, Oyo and Osun States. Agar well diffusion technique was used in the antimicrobial susceptibility testing. Toxicity testing was done using brine shrimps (Artemia salina). Biofilm-formers in this study are Klebsiella sp., Streptococcus sp., Staphylococcus sp., and Micrococcus sp. Ethanol leaf extracts had the highest activity against all biofilm-producing bacteria. Ethanol stem bark extract, which elicited activity against Klebsiella only, was found to be less toxic than the ethanol leaf extract. Staphylococcus showed >10 mm susceptibility to the ethanol and aqueous extracts of Macrosphyra longistyla. Streptococcus and Micrococcus were susceptible to the antimicrobial actions of the ethanolic leaf extracts. Although the ethanol extracts of the leaves had lower minimum inhibitory concentrations than the ethanol extracts of the stem bark, toxicity studies showed ethanol extracts of the stem-bark to be more toxic than the ethanol extracts of the leaves. In conclusion, ethanolic extracts of Macrosphyra longistyla show potential as sources of antimicrobials against gram-positive, oral biofilm-forming bacteria.

show abstract

Section: Identification Of Biofilm-producing Isolatesmentioning

confidence: 99%

Antimicrobial Activities of Extracts of <i>Macrosphyra longistyla</i> against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps

Nwankwo,

Folarin,

Osho

2024

AiM

View full text Add to dashboard Cite

show abstract

Genomic Insights Into Enterococcus mundtii 203: A Promising Probiotic Candidate Isolated From Camel Feces

Ahadaf,

Azzouz,

Galiou

et al. 2024

Probiotics & Antimicro. Prot.

View full text Add to dashboard Cite

Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens

Cited by 2 publications

References 53 publications

Antimicrobial Activities of Extracts of <i>Macrosphyra longistyla</i> against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps

Antimicrobial Activities of Extracts of <i>Macrosphyra longistyla</i> against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps

Genomic Insights Into Enterococcus mundtii 203: A Promising Probiotic Candidate Isolated From Camel Feces

Contact Info

Product

Resources

About

Genome sequencing of Enterococcus faecium NT04, an oral microbiota revealed the production of enterocin A/B active against oral pathogens

Cited by 2 publications

References 53 publications

Antimicrobial Activities of Extracts of &lt;i&gt;Macrosphyra longistyla&lt;/i&gt; against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps

Antimicrobial Activities of Extracts of &lt;i&gt;Macrosphyra longistyla&lt;/i&gt; against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps

Genomic Insights Into Enterococcus mundtii 203: A Promising Probiotic Candidate Isolated From Camel Feces

Contact Info

Product

Resources

About

Antimicrobial Activities of Extracts of <i>Macrosphyra longistyla</i> against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps

Antimicrobial Activities of Extracts of <i>Macrosphyra longistyla</i> against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps