Taeniasis-cysticercosis in Southern Ecuador: assessment of infection status using multiple laboratory diagnostic tools

Infection with versus Exposure to Taenia solium: What Do Serological Test Results Tell Us?

“…4 Briefly, a community-based study was conducted between September and November 2007 in the parish of Cazaderos, situated in the southern Andean province of Loja, where CC is endemic. 8 A total of 791 human serum samples were collected and tested using three serological methods, namely the enzymelinked immunosorbent assay (Ag-ELISA) for the detection of circulating antigens of the metacestode of T. solium , 9 the enzyme-linked immunoelectrotransfer blot assay (EITB) for the detection of antibodies directed against seven specific T. solium glycoprotein antigens, 10 and the ELISA for the detection of antibodies directed against crude cyst-fluid extracts (Ab-ELISA). 11 The results of the three tests applied on 791 individuals are shown in Table 1 .…”

“…Specificity of the Ag-ELISA for the detection of exposed individuals (th2 [3] in the model in Supplemental Appendix S1) 0.9-1 Probability to have a positive result for the EITB if the individual is exposed and positive for the Ag-ELISA (th2 [4] in the model in Supplemental Appendix S1) 0.95-1 Probability to have a positive result for the EITB if the individual is exposed and negative for the Ag-ELISA (th2 [5] in the model in Supplemental Appendix S1) 0.95-1 Probability to have a negative result for the EITB if the individual is not exposed and negative for the Ag-ELISA (th2 [6] in the model in Supplemental Appendix S1) 0.95-1 Probability to have a positive result for the Ab-ELISA if the individual is exposed and positive for the Ag-ELISA and the EITB (th2 [8] in the model in Supplemental Appendix S1) 0.9-1 Probability to have a negative result for the Ab-ELISA if the individual is exposed and negative for the Ag-ELISA and the EITB (th2 [12] in the model in Supplemental Appendix S1) 0.5-1 * The other probabilities are not constrained and left as uniform distributions (0-1). Ag-ELISA = enzyme-linked immunosorbent assay for the detection of circulating antigens of the metacestode of T. solium ; EITB = enzyme-linked immunoelectrotransfer blot assay for the detection of antibodies directed against seven specific T. solium glycoprotein antigens; Ab-ELISA = enzyme-linked immunosorbent assay for the detection of antibodies directed against crude cyst-fluid extracts of T. solium .…”

Complexities in using sentinel pigs to study Taenia solium transmission dynamics under field conditions

“…This highlights the importance of T. hydatigena as a cross-reacting species in the Ag-ELISA under study (as well as in other currently available antigen-ELISA tests; Krecek et al, 2008). In Asia and Latin America, T. hydatigena appears to be a common parasite of pigs, whereas in Africa it is frequently found in small ruminants but only occasionally in pigs (Dorny et al, 2004;Rodriguez-Hidalgo et al, 2006). Special care should therefore be taken while interpreting Ag-ELISA results obtained from Asian or Latin American pig samples.…”

“…The collected specimens were subjected to a polymerase chain reaction complemented with restriction fragment length assay (PCR-RFLP), as described by Rodriguez-Hidalgo et al (2006) and Somers et al (2007). In addition to the restriction enzymes DdeI and HinfI, which generate specific restriction patterns for T. solium, T. asiatica and Taenia saginata (Somers et al, 2007), the PCR fragments were also incubated with HpaI (New England Biolabs ® Inc.), which generates a specific restriction pattern for T. hydatigena.…”

Crude antigen from Taenia crassiceps cysticercus used as heterologous antigen in ELISA and in EITB for neurocysticercosis diagnosis of patients from Paraná-Brazil

“…In the same experiment, it was observed that the 15, 70, and 94 kDa antigens of T. solium were not specific, since they reacted with serum from negative control animals. Rodriguez-Hidalgo et al (2006) analyzed serum of infected swine and obtained high reactivity on 50 and 39-42 kDa protein bands. As reported by Molinari et al (2002), the indirect ELISA results showed that samples from patients with alive cysticercus had higher absorbance than samples from patients whose cysts were calcified or in transitional stages, but this distinction could not be made by immunoblotting.…”