Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis

Crude antigen from Taenia crassiceps cysticercus used as heterologous antigen in ELISA and in EITB for neurocysticercosis diagnosis of patients from Paraná-Brazil

“…Therefore, the use of this heterologous antigen may contribute for seroepidemiologic studies to evaluate the complex taeniasis/cysticercosis (Vaz et al, 1996;, Sciutto et al, 2000, Ito, 2000. Furthermore, studies have shown that immunoassays were able to detect whether the patients presented alive or inactive cysticerci (Molinari et al, 2002, Barcelos et al, 2005. In order to evaluate and confirm the epidemiological data of neurocysticercosis in the State of Paraná, Brazil, C. longicollis crude extract was used as heterologous antigen of C. cellulosae in ELISA and EITB assay of patients with different (Sotelo et al, 1988) diagnosed through neuroimages features.…”

“…The search for antigens is rare in neurocysticercosis diagnosis (Flisser and Gyorkos, 2007) and immunodiagnosis is based, primarily, on the detection of antibodies (Schantz and Tsang, 2003) by ELISA and EITB (Dua and Aneja, 2006;Schantz, 2006). Studies have shown it is possible by ELISA and using different antigens to discriminate between active and inactive NCC, i.e., when the cysticercus is alive or inert, respectively (Molinari et al, 2002;Barcelos et al, 2005). The aim of this work was to compare the experimental results obtained by ELISA and immunoblot assays using C. longicollis crude antigens and sera and cerebrospinal fluid (CSF) from patients with active, inactive NCC or with cysts in transitional phase, i.e., in degeneration, from the State of Paraná, Brazil.…”

Evaluation of an enzyme immunoassay for clinical diagnosis of neurocysticercosis in symptomatic patients

“…Several studies have shown that the performance of the ELISA method for diagnosis of neurocysticercosis is better in the stages of cysticercus degeneration [18][19][20] . These results are different from the present study, where six of the 15 patients with negative ELISA presented cysts in degeneration, while only one of the seven patients with positive ELISA presented cysts in degeneration.…”

“…Individuals with a greater number and size of lesions tested positive for ELISA of the CSF. One study showed that the presence of a single parenchymal lesion is related to low sensitivity, even with the Western blot method, which is the gold standard for serological diagnosis of neurocysticercosis 19 , suggesting some immune tolerance to the parasite and the absence of antibody production when only one cysts is present 17,18 .…”

“…Reviewing the literature, it appears that the ELISA method has shown different sensitivity and specificity scores [17][18][19][20][21][22][23][24][25][26][27][28] . This study determined a sensitivity of 31.8% and specificity of 100% for the ELISA method in CSF in relation to diagnosing neurocysticercosis, which implies low sensitivity of ELISA for diagnosing neurocysticercosis, with a 68.3% rate of false negative.…”