Saline extract of Taenia saginata metacestodes as an alternative antigen for the immunodiagnosis of neurocysticercosis in human cerebrospinal fluid

Oliveira, Heliana Batista de; Machado, Gleyce Alves; Gonçalves-Pires, Maria do Rosário F.; Moura, Leandro Pains; Costa-Cruz, Júlia Maria

doi:10.1007/s00436-009-1379-z

Cited by 11 publications

(14 citation statements)

References 30 publications

(32 reference statements)

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“…Furthermore, high sensitivity in ELISA and IB tests when using saline extract obtained from Taenia saginata metacestodes has already been demonstrated 18,19 .…”

Section: Discussionmentioning

confidence: 99%

“…Alternative antigens for NC immunodiagnosis continue to be a challenge because of the increasing difficulty in obtaining parasites from naturally infected pigs for preparation of Taenia solium homologous antigen 16 . Heterologous antigens from Taenia saginata have also been used with satisfactory results in ELISA and IB, and can be used as alternative antigens for diagnosing human NC [17][18][19] . Taenia saginata cysticerci in their cystic stage can be obtained in considerable quantities,…”

Section: Introductionmentioning

confidence: 99%

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Hydrophobic fraction of Taenia saginata metacestodes, rather than hydrophilic fraction, contains immunodominant markers for diagnosing human neurocysticercosis

Gonçalves

Machado

Oliveira

et al. 2010

Rev. Soc. Bras. Med. Trop.

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“…Furthermore, high sensitivity in ELISA and IB tests when using saline extract obtained from Taenia saginata metacestodes has already been demonstrated 18,19 .…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hydrophobic fraction of Taenia saginata metacestodes, rather than hydrophilic fraction, contains immunodominant markers for diagnosing human neurocysticercosis

Gonçalves

Machado

Oliveira

et al. 2010

Rev. Soc. Bras. Med. Trop.

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“…These new antigens have to be tested by various laboratories to prove that the sensitivity/specificity maintains between tests putting special attention on the selection of NCC patients and controls so the results can be compared. Some efforts have been done in recent years to try to make a more uniform immunodiagnostic test [20, 24] and to make comparable the results from different tests between laboratories [16, 26, 59, 60]. …”

Section: Problems Advances and Perspectives In The Immunodiagnosmentioning

confidence: 99%

“…Multiple antigens have been used for the immunodiagnosis of cysticercosis, among them are low molecular mass (LMM) antigens [31], excretory/secretory (ES) [30, 31, 71–74], crude soluble extract (CSE) [31, 68], total saline extract [59], antigen B [75, 76], lentil lectin glycoproteins (LLGPs) [52], vesicular fluid (VF) [68, 77], membrane and scolex extracts [68], somatic antigens [74], recombinant proteins [16–18, 20, 21, 25–28], and synthetic peptides [19, 24, 29]. The source of these antigens has been commonly Taenia solium (the parasite responsible for NCC) but related species as Taenia crassiceps [5, 24, 68, 77–79], Taenia saginata [59, 80], or Taenia taeniformis [81] have also been used as antigen sources; and among the multiple methods used to date for the immunodiagnosis of NCC complement fixation, agglutination, radioimmunoassays, ELISA and Western Blot (WB) can be counted [34, 77, 80, 82, 83].…”

Section: Antigens Used For Inmunodiagnosis Of Ncmentioning

confidence: 99%

Immunodiagnosis of Neurocysticercosis: Ways to Focus on the Challenge

Esquivel-Velázquez¹,

Ostoa‐Saloma²,

Morales‐Montor³

et al. 2011

BioMed Research International

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Neurocysticercosis (NCC) is a disease of the central nervous system that is considered a public health problem in endemic areas. The definitive diagnosis of this disease is made using a combination of tools that include imaging of the brain and immunodiagnostic tests, but the facilities for performing them are usually not available in endemic areas. The immunodiagnosis of NCC is a useful tool that can provide important information on whether a patient is infected or not, but it presents many drawbacks as not all infected patients can be detected. These tests rely on purified or semipurified antigens that are sometimes difficult to prepare. Recent efforts have focused on the production of recombinant or synthetic antigens for the immunodiagnosis of NCC and interesting studies propose the use of new elements as nanobodies for diagnostic purposes. However, an immunodiagnostic test that can be considered as “gold standard” has not been developed so far. The complex nature of cysticercotic disease and the simplicity of common immunological assumptions involved explain the low scores and reproducibility of immunotests in the diagnosis of NCC. Here, the most important efforts for developing an immunodiagnostic test of NCC are listed and discussed. A more punctilious strategy based on the design of panels of confirmed positive and negative samples, the use of blind tests, and a worldwide effort is proposed in order to develop an immunodiagnostic test that can provide comparable results. The identification of a set of specific and representative antigens of T. solium and a thorough compilation of the many forms of antibody response of humans to the many forms of T. solium disease are also stressed as necessary.

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“…In many countries where T. solium is endemic, neuroimaging methods could be inaccessible and/or too expensive for the population at risk, often rural. Under these conditions, serology may provide a viable tool for diagnosis of the infection (6,16,18,35).As NC immunodiagnosis continues to be a challenge because of the increasing difficulty in obtaining parasites from naturally infected pigs for the preparation of T. solium homologous antigen (33), alternative antigens, including heterologous antigens from Taenia saginata, have also been used with satisfactory results to diagnose human NC (29,31,32). T. saginata cysticerci in the cystic stage may be obtained in considerable amounts, because the eating habits in many countries, including Brazil, are based mainly on cattle meat consumption.…”

mentioning

confidence: 99%

Taenia saginata Metacestode Antigenic Fractions without Affinity to Concanavalin A Are an Important Source of Specific Antigens for the Diagnosis of Human Neurocysticercosis

Oliveira

Machado

Mineo

et al. 2010

Clin Vaccine Immunol

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Taenia saginata metacestode antigens have been constituted a useful alternative antigen for neurocysticercosis (NC) serodiagnosis, particularly due to an increasing difficulty to obtain Taenia solium homologous antigen. Cross-reactivity with Echinococcus granulosus infection occurs in homologous and heterologous antigens and could be avoided by using different purified methods. The present study evaluated antigen fractions obtained from saline extracts of T. saginata metacestodes purified by affinity chromatography with jacalin or concanavalin A (ConA) lectins to detect IgG antibodies by enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis to diagnose human NC. Serum samples were collected from 142 individuals: 40 of them were diagnosed with NC, 62 presented Taenia sp. and other parasites, and 40 were apparently healthy individuals. The jacalin-and ConA-unbound fractions demonstrated sensitivity and specificity higher than those of bound fractions. Among unbound fractions, ConA demonstrated statistically higher sensitivity and specificity by ELISA (90% and 93.1%, respectively). By immunoblot assay, the 64-to 68-kDa component from the ConA-unbound fraction showed 100% sensitivity and specificity, making this component suitable for use as a specific antigen for diagnosis of NC. To our knowledge, this is the first report showing the relevance of using the unbound ConA fraction of T. saginata metacestodes to diagnose NC. In conclusion, the results obtained herein clearly demonstrate that antigenic fractions without affinity to ConA, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC when tested by immunoblot assay.Taenia solium is a parasite whose larvae (metacestodes) may be located in the central nervous system of humans, causing neurocysticercosis (NC). Clinical manifestations are nonspecific and vary in severity, ranging from headaches, dizziness, and occasional seizures to a very severe neurological condition with intracranial hypertension or dementia (39,40,42).The diagnosis of NC is given by the combined analysis of clinical data and neuroimaging (computerized tomography and magnetic resonance imaging) as well as immunological and epidemiological data (10). From the neuroimaging findings of NC, only the presence of cystic lesions demonstrating the scolex should be considered pathognomonic (4). In many countries where T. solium is endemic, neuroimaging methods could be inaccessible and/or too expensive for the population at risk, often rural. Under these conditions, serology may provide a viable tool for diagnosis of the infection (6,16,18,35).As NC immunodiagnosis continues to be a challenge because of the increasing difficulty in obtaining parasites from naturally infected pigs for the preparation of T. solium homologous antigen (33), alternative antigens, including heterologous antigens from Taenia saginata, have also been used with satisfactory results to diagnose human NC (29,31,32). T. saginata cysticerci in the cysti...

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Saline extract of Taenia saginata metacestodes as an alternative antigen for the immunodiagnosis of neurocysticercosis in human cerebrospinal fluid

Cited by 11 publications

References 30 publications

Hydrophobic fraction of Taenia saginata metacestodes, rather than hydrophilic fraction, contains immunodominant markers for diagnosing human neurocysticercosis

Hydrophobic fraction of Taenia saginata metacestodes, rather than hydrophilic fraction, contains immunodominant markers for diagnosing human neurocysticercosis

Immunodiagnosis of Neurocysticercosis: Ways to Focus on the Challenge

Taenia saginata Metacestode Antigenic Fractions without Affinity to Concanavalin A Are an Important Source of Specific Antigens for the Diagnosis of Human Neurocysticercosis

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