Evaluation of indirect susceptibility testing of Mycobacterium tuberculosis to the first- and second-line, and alternative drugs by the newer MB/BacT system

Barreto, Angela Maria Werneck; Araújo, Joselba; Medeiros, Reginalda Ferreira de Melo; Caldas, Paulo César de Souza

doi:10.1590/s0074-02762003000600020

Cited by 11 publications

(16 citation statements)

References 8 publications

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“…However, adequate data and more studies are required to make such a generalization or recommendation. The sensitivity and the specificity of the BacT/ALERT 3D system in the present study are comparable to those in earlier reports (2,4,29). The BacT/ALERT 3D system was shown to be rapid as results were available after 10.92 (median, 10.32) days on average (range, 2.57 to 20.29 days).…”

Section: Discussionsupporting

confidence: 89%

“…Using LJ proportional susceptibility testing results as the gold standard (9), the performance parameters of the BacT/ALERT 3D system and the PZase assay were determined as recommended by Enarson et al (10) and also as described by Barreto et al (2). Fisher's exact test (two-tailed) at a 5% level of significance was applied to determine the statistical significance of the difference between the sensitivities of both of these tests.…”

Section: Resolution Of Discordancementioning

confidence: 99%

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Comparative Evaluation of Löwenstein-Jensen Proportion Method, BacT/ALERT 3D System, and Enzymatic Pyrazinamidase Assay for Pyrazinamide Susceptibility Testing of Mycobacterium tuberculosis

Singh¹,

Wesley²,

Jadaun³

et al. 2007

J Clin Microbiol

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Pyrazinamide (PZA) is an important first-line antituberculosis drug because of its sterilizing activity against semidormant tubercle bacilli. In spite of its very high in vivo activity, its in vitro activity is not apparent unless an acidic environment is available, which makes PZA susceptibility testing difficult by conventional methods. The present study was, therefore, planned to assess the performance of the colorimetric BacT/ALERT 3D system and compare the results with those from conventional tests, i.e., the Löwenstein-Jensen (LJ) proportion method (pH 4.85) and Wayne's pyrazinamidase (PZase) assay, using 107 clinical isolates. The concordance among all of these tests was 89.71% after the first round of testing and reached 92.52% after resolution of the discordant results by retesting. Prolonged incubation of the PZase tube for up to 10 days was found to increase the specificity of the PZase test. The concordances between LJ proportion and BacT/ALERT 3D, LJ proportion and the PZase assay, and BacT/ALERT 3D and the PZase assay were found to be 99.06%, 93.46%, and 92.52%, respectively. Using the LJ results as the gold standard, the sensitivities of BacT/ALERT 3D and the PZase assay were 100 and 82.85%, respectively, while the specificity was 98.61% for both of the tests. The difference between the sensitivities of BacT/ALERT 3D and the PZase assay was significant (P ‫؍‬ 0.025). The mean turnaround times for the detection of resistant and susceptible results by BacT/ALERT 3D were 8.04 and 11.32 days, respectively. While the major limitations associated with the PZase assay and the LJ proportion method are lower sensitivity in previously treated patients and a longer time requirement, respectively, the BacT/ ALERT 3D system was found to be rapid, highly sensitive, and specific.

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Section: Discussionsupporting

confidence: 89%

Section: Resolution Of Discordancementioning

confidence: 99%

Comparative Evaluation of Löwenstein-Jensen Proportion Method, BacT/ALERT 3D System, and Enzymatic Pyrazinamidase Assay for Pyrazinamide Susceptibility Testing of Mycobacterium tuberculosis

Singh¹,

Wesley²,

Jadaun³

et al. 2007

J Clin Microbiol

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“…AST was performed by the conventional proportion method using LJ medium and automation in the BacT Alert 3D system [6,9,10,11,12,13,14,15,16]. All the LJ subcultures of Mycobacteria were ≤3 weeks old and ≤36 hours, in the BacT/ Alert MP bottle.…”

Section: Antimicrobial Susceptibility Testingmentioning

confidence: 99%

“…The stock solutions were stored in small aliquots at -70°C. Working solution (1000µg/mL) was prepared on the day of the tests and added to the LJ medium and MB Bact medium to achieve the critical concentration (Table 1) [2,6,9,[11][12][13][14][15]. At least 3 critical concentration of each of the drugs were tested in the BacT Alert 3D system to determine the ideal concentration for the strains prevailing in the local environment Pfyffer et al 1999).…”

Section: Antimicrobial Agentsmentioning

confidence: 99%

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Standardization of first and second-line antitubercular susceptibility testing using BacT Alert 3D system: a report from a tertiary care centre in India

et al. 2009

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Resurgence of multidrug resistant tuberculosis has lead to demand for rapid susceptibility testing. Conventional methods take > 3 weeks and are tedious. Automated methods have superseded them for first line drug susceptibility testing. An attempt was made to standardize first and second line susceptibility testing using the BacT Alert 3D system (Biomerieux). And compare results with Lowenstein Jensen's (LJ) method. 121 isolates of Mycobacterium tuberculosis, 67 pulmonary and 54 extra pulmonary were subjected to sensitivity to first and second line drugs. Multidrug resistance was detected equally by both methods at 15.7%. 100% agreement was observed between the two methods for aminoglycosides, rifampicin, ethionamide and ciprofloxacin. 91.5% agreement was observed for isoniazid, 85% for pyrazinamide and 72.4% for ethambutol. The time taken by LJ method was 18-32 days and BacT Alert 3D system took 4-12 days. In the lesser developed nations where tuberculosis is rampant a rapid effective method for confirming multidrug resistant tuberculosis is definitely desirable and the BacT Alert 3D system was found an effective method when compared to the 'gold standard' LJ proportion.

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Microscopic-Observation Drug-Susceptibility Assay for the Diagnosis of TB

et al. 2006

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Evaluation of indirect susceptibility testing of Mycobacterium tuberculosis to the first- and second-line, and alternative drugs by the newer MB/BacT system

Abstract: , ; and 100% specificity for AMI (85.4-100), , and compared to the BACTEC system. Comparing the results obtained in LJ we observed 100% sensitivity for RMP , followed by , , and 100% specificity for all drugs tested except for

Cited by 11 publications

References 8 publications

Comparative Evaluation of Löwenstein-Jensen Proportion Method, BacT/ALERT 3D System, and Enzymatic Pyrazinamidase Assay for Pyrazinamide Susceptibility Testing of Mycobacterium tuberculosis

Comparative Evaluation of Löwenstein-Jensen Proportion Method, BacT/ALERT 3D System, and Enzymatic Pyrazinamidase Assay for Pyrazinamide Susceptibility Testing of Mycobacterium tuberculosis

Standardization of first and second-line antitubercular susceptibility testing using BacT Alert 3D system: a report from a tertiary care centre in India

Microscopic-Observation Drug-Susceptibility Assay for the Diagnosis of TB

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