Microscopic-Observation Drug-Susceptibility Assay for the Diagnosis of TB

Moore, David; Evans, Carlton A.; Gilman, Robert H.; Caviedes, Luz; Coronel, Jorge; Vivar, Aldo; Sánchez, Eduardo; Piñedo, Yvette; Saravia, Juan Carlos; Salazar, Cayo; Oberhelman, Richard A.; Hollm-Delgado, Maria Graciela; LaChira, Doris; Escombe, A. Roderick; Friedland, Jon S.

doi:10.1056/nejmoa055524

Cited by 423 publications

(392 citation statements)

References 29 publications

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“…Second-line DST with MGIT is being optimised at several European Supranational labs. Apart from this, there are other poor man's options such as microscopic observation broth-drug susceptibility assay (MODS), Slide DST, Phage-based assays, Colorimetric methods with MTT and resazurin as redox indicators and Nitrate reductase assay [17][18][19][20][21][22].…”

Section: Phenotypic Methodsmentioning

confidence: 99%

True facets of TB diagnosis in 2012: Hypes and realities

Parida¹

2012

European Journal of Microbiology and Immunology

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Tuberculosis takes a heavy toll of ~5000 lives every day from the disease; responsible for the 86% of DALY burden. Despite having drugs to treat TB efficiently, we have failed to control the disease. Mycobacterium tuberculosis has exploited it to their advantage evolving with multiple mutations making it resistant to first-line and second-line drugs. Most of the high-burden countries are lowmedium income countries, their national TB program (NTP) still use sputum smear microscopy as the tool of diagnosis. Many new molecular tools are emerging, but confuse the larger TB clinical scientific community at the NTPs. Coherent information need to be disseminated, encouraging TB scientific community to generate evidences within NTPs assessing new tools through critical analyses in terms of value addition and cost benefit before considering rolling out in the program. It is also imperative that the scientific community need to have an open mind to use different tools in the right permutation and combination than being exclusive of one another. This article portrays an overview of the diagnostics landscape in 2012 with pros and cons of different tools to be able to generate a step-wise algorithm for optimal exploitation of the tools within available resources in each of the settings.

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Section: Phenotypic Methodsmentioning

confidence: 99%

True facets of TB diagnosis in 2012: Hypes and realities

Parida¹

2012

European Journal of Microbiology and Immunology

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“…Growth in the drug-containing wells and in the growth control is interpreted as resistance. MODS has been evaluated against first-line drugs although better results have been obtained for RIF and INH (Moore et al, 2004(Moore et al, , 2006. Although fairly accurate to detect resistance, especially to RIF, the requirement of an inverted microscope to read the plates and biosafety concerns related to the use of liquid medium in 24-well plates may hamper, however, its application outside large reference laboratories with the necessary logistic facilities.…”

Section: The Microscopic Observation Broth-drug Susceptibility Assay mentioning

confidence: 99%

Rapid culture-based methods for drug-resistance detection in Mycobacterium tuberculosis

Palomino

Martin

Groll

et al. 2008

Journal of Microbiological Methods

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“…give an estimated incidence figure of 2.2 million cases of TB for India out of a global [3] Diagnosis is confirmed by microscopy examination and culture for growth of mycobacterium tuberculosis in developing countries because of low cost.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Rapid Slide Culture and Conventional Lj Method for Detection of Tb From Suspected Cases of Tuberculosis

Patil¹

2017

WJPR

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Tuberculosis is one of the dangerous disease in the world and its spreading very fast. There is emergence Drug resistance TB (MDR, XDR and TDR) because of many reasons. Transmission of MDR and XDR will be curse to human being. The objective of this study is to compared the Rapid slide culture (RSC) method with the growth on Lowenstein Jensen(LJ) media and to know the sensitivity and specificity of the rapid slide culture method. Total 100 sample was collected from suspected pulmonary tuberculosis cases. All the sample were subjected to three test:1.Zeihl Neelsen Staining 2. Rapid slide culture 3. Culture on Lowenstein Jensen media and the result were compared. Of the total 100 suspected sputum samples which were tested by all three methods, 57 samples were found to be Smear positive, 64 were found to be positive by the RSC method, 68 samples were found to be positive by the LJ culture method. The sensitivity of RSC was 91.17% specificity was 93.75%. Diagnosis can be done within 1 week with RSC where as 3-4 weeks by Conventional culturing method. So this method can be used in routine and especially in patient who have symptom of TB and have Negative smear of acid fast bacteria.

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Microscopic-Observation Drug-Susceptibility Assay for the Diagnosis of TB

Abstract: Please refer to usage guidelines at http://researchonline.lshtm.ac.uk/policies.html or alternatively contact researchonline@lshtm.ac.uk. Available under license: Copyright the publishersT h e ne w e ngl a nd jou r na l o f m e dic i ne n engl j med 355;15 www.nejm

Cited by 423 publications

References 29 publications

True facets of TB diagnosis in 2012: Hypes and realities

True facets of TB diagnosis in 2012: Hypes and realities

Rapid culture-based methods for drug-resistance detection in Mycobacterium tuberculosis

Comparison of Rapid Slide Culture and Conventional Lj Method for Detection of Tb From Suspected Cases of Tuberculosis

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