Standardization of first and second-line antitubercular susceptibility testing using BacT Alert 3D system: a report from a tertiary care centre in India

Abstract

Resurgence of multidrug resistant tuberculosis has lead to demand for rapid susceptibility testing. Conventional methods take > 3 weeks and are tedious. Automated methods have superseded them for first line drug susceptibility testing. An attempt was made to standardize first and second line susceptibility testing using the BacT Alert 3D system (Biomerieux). And compare results with Lowenstein Jensen's (LJ) method. 121 isolates of Mycobacterium tuberculosis, 67 pulmonary and 54 extra pulmonary were subjected to sensitivity to first and second line drugs. Multidrug resistance was detected equally by both methods at 15.7%. 100% agreement was observed between the two methods for aminoglycosides, rifampicin, ethionamide and ciprofloxacin. 91.5% agreement was observed for isoniazid, 85% for pyrazinamide and 72.4% for ethambutol. The time taken by LJ method was 18-32 days and BacT Alert 3D system took 4-12 days. In the lesser developed nations where tuberculosis is rampant a rapid effective method for confirming multidrug resistant tuberculosis is definitely desirable and the BacT Alert 3D system was found an effective method when compared to the 'gold standard' LJ proportion.

Antimicrobial Resistance Pattern of Mycobacterium Tuberculosis Complex Isolated from Extrapulmonary Tuberculosis Patientsin Sohag Governorate

“…Meanwhile, the highest resistance rates were to Isoniazid (13.2%) ,Ofloxacin(11.3%), Rifampicin(9.4%), Amikacin(7.5%). In another study by Nair et al (2009) showed resistance pattern of MTBC extrapulmonary towards first and second line drugs by LJ proportion method, it supported our result that the strains had highest resistance to isoniazid 16/54 (29.6%) and highest sensitivity to ethambutol but it differ from our result in the following resistance after isoniazid. It followed by rifampcin 8/54 (14.8%), pyrazinamide 7/54(12.9%), ethionamide, kanamycin and capreomycin (each 6/54represented 11.1%) then ciprofloxacin and amikacin(each 5/54 represented 9.2%) then ethambutol 4/54(7.4%).Also Pollett et al (2016) reporteddrugsusceptibility testing results (by phenotypic or genotypic methods) which were lower than our result.…”

Anti-Tuberculosis Activity of Crude Ethanol Extracts of Mangrove Rhizosphere Fungi, Trichoderma Spp.

“…[14] MGIT is an rapid effective method for confirming multidrug resistant tuberculosis and it was found an effective method when compared to the 'gold standard' conventional method. [15] Addition of antibiotics will minimize the contamination rate and improve the result than the conventional method. [16] The studies of volatile metabolites are necessary to determine the secondary metabolites and are highly involved in the multifaceted interactions between filamentous fungi and their living environment.…”

SAR studies on 1,2,4-triazolo[3,4-b][1,3,4]thiadiazoles as inhibitors of Mtb shikimate dehydrogenase for the development of novel antitubercular agents

“…RDRTB using the BacT/ALERT 3D liquid culture technology. 7 The antitubercular activities of the compounds are indicated by MIC values. Results are summarized in Table 1.…”

“…80, 000 compounds have been screened by using this model and evaluated for their activity against Mtb H37Rv, MDRTB (isoniazid and rifampin resistant strains) and RDRTB (rifampin resistant strains) initially using the BacT/ALERT 3D (MB/BacT) liquid culture technology. 7 One 3,6-disubstituted triazolothiadiazole (1, MIC-H37Rv ¼ 8.0 mg mL À1 ; MIC-MDRTB ¼ 8.0 mg mL À1 ; MIC-RDRTB ¼ 8.0 mg mL À1 ; Mt SD-IC 50 ¼ 23.00 mg mL À1 ) has been identied as a promising lead for antitubercular drug development.…”