Serodiagnosis of chronic Chagas infection by using EIE-Recombinant-Chagas-Biomanguinhos kit

Gomes, Yara M.; Pereira, Valéria Rêgo Alves; Nakazawa, Mineo; Rosa, Daniela Santoro; Barros, Maria das Neves D.S; Ferreira, Antônio G. P.; Silva, Edimilson D.; Ogatta, Sueli Fumie Yamada; Krieger, Marco Aurélio; Goldenberg, Samuel

doi:10.1590/s0074-02762001000400009

Cited by 39 publications

(38 citation statements)

References 14 publications

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“…14 Indeed, an Argentine study in a co-endemic area for Chagas disease and leishmaniasis showed 81.8% (9/11) cross-reactivity by using conventional ELISA 27 and a relative large number of false-positive or inconclusive results have been reported using conventional serologic testing. 13,28,29 Imuno-ELISA Chagas and Pathozyme Chagas use recombinant proteins as antigen. As has already been shown, this approach proved to be successful, with improvement of Sp and Se.…”

Section: Discussionmentioning

confidence: 99%

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Chronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Tests

Santos¹,

Souza²,

Barros³

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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Abstract. There is a significant heterogeneity in reported performance of serological assays for Chagas disease diagnosis. The conventional serology testing in laboratory diagnosis and in blood banks is unsatisfactory because of a high number of inconclusive and misclassified results. We aimed to assess the quality of four commercially available enzymelinked immunosorbent assay tests for their ability to detect Trypanosoma cruzi antibodies in 685 sera samples. Crossreactivity was assessed by using 748 sera from patients with unrelated diseases. Initially, we found that the reactivity index against T. cruzi antigen was statistically higher in sera from Chagas disease patients compared with those from non-chagasic patients, supporting the notion that all evaluated tests have a good discriminatory ability toward the diagnosis of T. cruzi infection in patients in the chronic phase of the disease. Although all tests were similarly sensitive for diagnosing T. cruzi infection, there were significant variations in terms of specificity and cross-reactivity among them. Indeed, we obtained divergent results when testing sera from patient with unrelated diseases, particularly leishmaniasis, with the levels of cross-reactivity being higher in tests using whole T. cruzi extracts compared with those using recombinant proteins. Our data suggest that all four tests may be used for the laboratory diagnosis and routine blood screening diagnose for Chagas disease. We also emphasize that, despite their general good performance, caution is needed when analyzing the results when these tests are performed in areas where other diseases, particularly leishmaniasis, are endemic.

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Section: Discussionmentioning

confidence: 99%

“…As it could be expected, there is considerable variation in the reproducibility, feasibility, and reliability of such positive control samples. 13 Most worrisome is the reported cross-reactivity with related protozoan parasites, particularly Leishmania spp. and Trypanosoma rangeli.…”

Section: Introductionmentioning

confidence: 99%

Chronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Tests

Santos¹,

Souza²,

Barros³

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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“…Four methodologies of distinct principles were used, including inhouse conventional ELISA test (ELISA), recombinant-ELISA (EIERec-ELISA) using CRA+FRA 17,18 T. cruzi recombinat antigens, Indirect Immunofluorescence test (IIF), both from BIO-Manguinhos and Indirect Hemaglutination assay (IHA) from Immunoserum. The ELISA test was performed using the standardized methodology described above for BEFP screening tests.…”

Section: Confirmatory Serological Testsmentioning

confidence: 99%

Seroprevalence of Chagas disease in schoolchildren from two municipalities of Jequitinhonha Valley, Minas Gerais, Brazil; six years following the onset of epidemiological surveillance

Borges

Assis

Gomes

et al. 2006

Rev. Inst. Med. trop. S. Paulo

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SUMMARYSix years after the beginning of the epidemiological surveillance of Chagas disease in Berilo and José Gonçalves de Minas, Jequitinhonha Valley, MG, Brazil, a serological inquiry was performed to observe whether the transmission of this endemy was occurring in this area. A randomized sample of 1,412 children seven to 14 years old, was screened. Six asymptomatic children were found to be positive, leading to 0.4% of prevalence. Hemoculture confirmed infection in five out of the six positive cases. Additional epidemiological investigation revealed important antecedents, such as disease reports in relatives and predisposing ecological and housing conditions. Our results demonstrated similar seroprevalence (0.4%) in schoolchildren, ranging from seven to 14 years old, and that were observed six years ago (0.2%) for children 0-9 year-old. Thus, considering the constant presence of Panstrogylus megistus in the peridomicile these findings emphasize the need of continuous improved epidemiological surveillance of Chagas disease in this region.

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“…CRA (cytoplasmic repetitive antigen) and FRA (flagellar repetitive antigen) recombinant antigens (RecAg) of T. cruzi have been used, successfully in serodiagnosis of Chagas disease (Krieger et al 1992, Gomes et al 2001. FRA is located in the flagellum of the epimastigote and trypomastigote forms of T. cruzi and displays a 68-amino acid repeat, while CRA is distributed throughout the cytoplasm of epimastigote and amastigote forms and has a 14-amino acid repeat (Lafaille et al 1989 The response of chagasic patients to these antigens (Krieger et al 1992, Gomes et al 2001) led us to investigate their immunologial response in experimental animals.…”

mentioning

confidence: 99%

“…FRA is located in the flagellum of the epimastigote and trypomastigote forms of T. cruzi and displays a 68-amino acid repeat, while CRA is distributed throughout the cytoplasm of epimastigote and amastigote forms and has a 14-amino acid repeat (Lafaille et al 1989 The response of chagasic patients to these antigens (Krieger et al 1992, Gomes et al 2001) led us to investigate their immunologial response in experimental animals.…”

mentioning

confidence: 99%

Antibody isotype responses in Balb/c mice immunized with the cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi

Pereira

Lorena

Verçosa

et al. 2003

Mem. Inst. Oswaldo Cruz

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Serodiagnosis of chronic Chagas infection by using EIE-Recombinant-Chagas-Biomanguinhos kit

Abstract: A kit based on an enzyme immunoassay, EIE-Recombinant-Chagas-Biomanguinhos, developed (syphilis, n=8; HTLV, n=8; HCV, n=7 and HBV, n=12)

Cited by 39 publications

References 14 publications

Chronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Tests

Chronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Tests

Seroprevalence of Chagas disease in schoolchildren from two municipalities of Jequitinhonha Valley, Minas Gerais, Brazil; six years following the onset of epidemiological surveillance

Antibody isotype responses in Balb/c mice immunized with the cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi

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