Antibody isotype responses in Balb/c mice immunized with the cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi

Pereira, Valéria Rêgo Alves; Lorena, Virgínia Maria Barros de; Verçosa, Alinne Fernanda Amaral; Silva, Edmilson D.; Ferreira, Antônio G. P.; Montarroyos, Ulísses Ramos; Silva, Ana P. Galvao; Gomes, Yara M.

doi:10.1590/s0074-02762003000600019

Cited by 12 publications

(12 citation statements)

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“…CRA and FRA present repetitive structures and were capable of inducing specific antibodies in BALB/c and C57BL/6 mice even in the absence of T. cruzi infection (Pereira et al 2003a(Pereira et al , 2003b. In the present paper, although antibodies involved in the elimination of the parasites, as reported by Pereira et al (2003aPereira et al ( , 2003b, were detected; and despite the survival of a significant number of immunized infected mice when compared with the control group, the humoral immune response was not enough to provide full protection against T. cruzi at 30 d.p.i.…”

Section: Discussionmentioning

confidence: 99%

“…These antigens were able to elicit the production of specific IgG immunoglobulins. CRA induced predominantly the production of IgG1 and IgG3 isotypes in BALB/c and C57BL/6 mice, while FRA induced antibodies of the IgG1 isotype in BALB/c mice (Pereira et al 2003a(Pereira et al , 2003b. Cellular lymphoproliferative response was also observed in both mouse strains.…”

Section: Introductionmentioning

confidence: 88%

“…We have previously demonstrated the immunogenicity of cytoplasmic repetitive antigen (CRA) and flagellar repetitive antigen (FRA) recombinant proteins of T. cruzi by evaluating the humoral and cellular responses in uninfected susceptible (BALB/c) and resistant (C57BL/6) mice strains immunized with these proteins (Pereira et al 2003a(Pereira et al , 2003b(Pereira et al , 2004. These antigens were able to elicit the production of specific IgG immunoglobulins.…”

Section: Introductionmentioning

confidence: 99%

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Humoral and cellular immune responses in BALB/c and C57BL/6 mice immunized with cytoplasmic (CRA) and flagellar (FRA) recombinant repetitive antigens, in acute experimental Trypanosoma cruzi infection

et al. 2005

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In previous studies, cytoplasmic repetitive antigen (CRA) and flagellar repetitive antigen (FRA) proteins induced specific humoral and cellular immune responses in susceptible and resistant mice in the absence of Trypanosoma cruzi infection with a significant induction of the Interferon-gamma (IFN-gamma) production in those animals. In this follow-up paper, the immunostimulatory and protective effects of these proteins were evaluated by immunizing with CRA or FRA antigens, BALB/c and C57BL/6 mice and challenging with a T. cruzi (Y strain). Both proteins induced humoral response with high levels of IgG isotypes as well as cellular immunity with high levels of IFN-gamma when compared to controls. However, the lymphocyte proliferative response was minimal. The survival rate at 30 days post-infection was significant in CRA (60%) or FRA (50%)--immunized BALB/c mice and CRA (83.3%)--immunized C57BL/6 mice. Taken as a whole these findings indicate that CRA and FRA are immunogenic and potentially important for protective immunity.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Humoral and cellular immune responses in BALB/c and C57BL/6 mice immunized with cytoplasmic (CRA) and flagellar (FRA) recombinant repetitive antigens, in acute experimental Trypanosoma cruzi infection

et al. 2005

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“…CRA-immunized mice produced high levels of all IgG isotypes, mainly IgG3 and IgG1. FRA-immunization elicited only high levels of IgG1 [38]. With co-adsorbed to alum and recombinant murine IL-12, recombinant Paraflagellar Rod proteins (PFR), which are conserved among seven highly diverse strain of T. cruzi, induced protective immunity against T. cruzi [39].…”

Section: Subunit Experimental Vaccinesmentioning

confidence: 99%

Strategy for the Development of Vaccines Against Chagas Disease

Huang¹,

Campus²

2015

VRV

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“…Two recombinant antigens (Rec-Ags), cytoplasmatic repetitive antigen (CRA) and flagellar repetitive antigen (FRA), already studied in murine model (26)(27)(28)(29), have been successfully used in the immunodiagnosis of Chagas' disease (30)(31)(32)(33). These antigens were also used to evaluate the cure for Chagas' disease in patients from Minas Gerais (Brazil) treated in the acute phase of the infection (34).…”

mentioning

confidence: 99%

Cellular immune response from chagasic patients to CRA or FRA recombinant antigens of Trypanosoma cruzi

Lorena

Verçosa

Machado

et al. 2008

Clinical Laboratory Analysis

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We propose to analyze the relation between the cellular immune response of Chagas' disease patients after in vitro stimulation of peripheral blood mononuclear cells (PBMC) with recombinant antigens cytoplasmatic repetitive antigen (CRA) or flagellar repetitive antigen (FRA) of T. cruzi and the chronic clinical forms of disease. Cells were stimulated using phytohemagglutinin, CRA, FRA, or a soluble antigen of Epimastigota (Ag-Epi) for 24 hr, 72 hr, or 6 days. The proliferation of cells was evaluated after 6 days of culture by quantification of incorporated 3H-thymidine. Cytokines were measured in the supernatants obtained after 24 hr (tumor necrosis factor [TNF]-alpha and interleukin [IL]-4), 72 hr (IL-10), and 6 days (interferon [IFN]-gamma) using enzyme-linked immunosorbent assay (ELISA). Cells of the Chagas patients stimulated with the recombinant antigens exhibited higher proliferation responses compared with that of non-Chagas (NC) individuals. However, when proliferation was compared between patients with the cardiac form (CF) or indeterminate form (IF), it was not possible to establish a difference in the response. So far as the cytokines secreted in the culture supernatants after stimulation in vitro with T. cruzi antigens were concerned, the results showed that CRA, as well as Epi-Ag, were able to stimulate the production of TNF-alpha and IFN-gamma in Chagas patients as compared with NC individuals. However, the cytokine levels after stimulation with the T. cruzi antigens were not different between the patients with CF and IF. CRA was capable of inducing a T helper type 1 (Th1) immune response, with elevated production of TNF-alpha and IFN-gamma in Chagas patients that are carriers of CF and IF clinical forms.

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Antibody isotype responses in Balb/c mice immunized with the cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi

Abstract: In the present report we analyzed the levels of IgG1, IgG2a, IgG2b and

Cited by 12 publications

References 10 publications

Humoral and cellular immune responses in BALB/c and C57BL/6 mice immunized with cytoplasmic (CRA) and flagellar (FRA) recombinant repetitive antigens, in acute experimental Trypanosoma cruzi infection

Humoral and cellular immune responses in BALB/c and C57BL/6 mice immunized with cytoplasmic (CRA) and flagellar (FRA) recombinant repetitive antigens, in acute experimental Trypanosoma cruzi infection

Strategy for the Development of Vaccines Against Chagas Disease

Cellular immune response from chagasic patients to CRA or FRA recombinant antigens of Trypanosoma cruzi

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