Prostaglandin A1 Inhibits Replication of Classical Swine Fever Virus

Freitas, Tânia Rosária Pereira; Caldas, Lúcio Ayres; Rebello, M. A.

doi:10.1590/s0074-02761998000600021

Cited by 4 publications

(4 citation statements)

References 14 publications

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“…Chemical analysis of these PGs indicated that the presence of a carbonyl group at the unsaturated (α, β) cyclopentane ring might be responsible for this property 21,22 . PGs inhibit production of MAYV 23 and other viruses, including Sendai 24 , Vesicular stomatitis virus (VSV) 25,26 , human immunodeficiency virus (HIV) 27 , Rotavirus 28 and Classical swine fever virus 29 .…”

Section: Introductionmentioning

confidence: 99%

Prostaglandin A1 triggers Mayaro virus inhibition and heat shock protein 70 expression in an epithelial cell model

Caldas

Ferreira

Freitas

2018

Rev. Soc. Bras. Med. Trop.

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Introduction: The Mayaro virus (MAYV), which is an arbovirus closely related to the Chikungunya virus, causes a dengue-like acute illness that is endemic to Central and South America. We investigated the anti-MAYV activity of prostaglandin A 1 (PGA 1), a hormone which exhibits antiviral activity against both ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) viruses. Further, we examined the effects of inducting the stress protein HSP70 following PGA 1 treatment. Methods: Hep-2 cells infected with MAYV were treated with PGA 1 (0.1-6μg/ml) 12h before infection and for different periods post-infection. Inhibition of viral replication inhibition was analyzed via viral titer determination, whereas the effect of PGA 1 on viral morphogenesis was examined via transmission electron microscopy (TEM). Autoradiography (with 35 S methionine labeling) and western blotting were used to assess the effect of PGA 1 treatment on viral and cellular protein synthesis, and on HSP70 induction, respectively. Results: PGA 1 strongly reduced viral replication in Hep-2 cells, particularly when added during the early stages of viral replication. Although PGA 1 treatment inhibited viral replication by 95% at 24 hours post-infection (hpi), viral structural protein synthesis was inhibited only by 15%. TEM analysis suggested that PGA 1 inhibited replication before viral morphogenesis. Western blot and densitometry analyses showed that PGA 1 treatment increased HSP70 protein levels, although this was not detectable via autoradiography. Conclusions: PGA 1 inhibits MAYV replication in Hep-2 cells at early stages of viral replication, prior to production of viral structural proteins, possibly via HSP70 induction.

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Section: Introductionmentioning

confidence: 99%

Prostaglandin A1 triggers Mayaro virus inhibition and heat shock protein 70 expression in an epithelial cell model

Caldas

Ferreira

Freitas

2018

Rev. Soc. Bras. Med. Trop.

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“…Prostaglandin A 1 (PGA 1 ) is capable of blocking the replication of a wide variety of RNA and DNA viruses, including CSFV. 9 , 10 , 11 Despite the importance of BVDV for animal health, the effect of PGA 1 on BVDV replication has not been examined. Therefore, we evaluate the effect of PGA 1 on the replication of cpBVDV in MDBK cells, focusing on changes in BVDV-induced vacuolization.…”

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confidence: 99%

“…Although prostaglandins (PGs) are capable of inhibiting the replication of a variety of RNA and DNA viruses, their mode of action is not entirely clear. 9 , 10 , 11 In some systems, PGAs exert potent antiviral activity by inducing the synthesis of proteins from the heat shock protein 70 (HSP70) family, through cycloheximide-sensitive activation of a heat-shock trigger factor. 13 …”

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confidence: 99%

Prostaglandin A1 inhibits the replication of bovine viral diarrhea virus

Caldas

Freitas

Azevedo

et al. 2018

Brazilian Journal of Microbiology

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Bovine viral diarrhea virus can cause acute disease in livestock, leading to economic losses. We show that Prostaglandin A1 inhibits bovine viral diarrhea virus replication in Madin-Darby bovine kidney cells (94% inhibition using 5 μg/mL). Light and electron microscopy of infected cells shows that Prostaglandin A1 also prevents virus-induced vacuolization, but at higher concentrations (10 μg/mL).

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“…MG132 [119], a proteasome inhibitor, could attenuate the CSFV replication. (9) Other molecules also have anti-CSFV effects, such as Prostaglandin A1 [120,121], the phage-displayed E2-binding peptides [122], ceramide (C6) (activator of the protein phosphatase 1 pathway) [123], and quercetin (inhibitor of HSP70 function) [88]. More and more anti-CSFV drug candidates will be discovered with the increase of antiviral research.…”

Section: Drug Candidates With Anti-csfv Effectsmentioning

confidence: 99%

Anti-Classical Swine Fever Virus Strategies

et al. 2021

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Classical swine fever (CSF), caused by CSF virus (CSFV), is a highly contagious swine disease with high morbidity and mortality, which has caused significant economic losses to the pig industry worldwide. Biosecurity measures and vaccination are the main methods for prevention and control of CSF since no specific drug is available for the effective treatment of CSF. Although a series of biosecurity and vaccination strategies have been developed to curb the outbreak events, it is still difficult to eliminate CSF in CSF-endemic and re-emerging areas. Thus, in addition to implementing enhanced biosecurity measures and exploring more effective CSF vaccines, other strategies are also needed for effectively controlling CSF. Currently, more and more research about anti-CSFV strategies was carried out by scientists, because of the great prospects and value of anti-CSFV strategies in the prevention and control of CSF. Additionally, studies on anti-CSFV strategies could be used as a reference for other viruses in the Flaviviridae family, such as hepatitis C virus, dengue virus, and Zika virus. In this review, we aim to summarize the research on anti-CSFV strategies. In detail, host proteins affecting CSFV replication, drug candidates with anti-CSFV effects, and RNA interference (RNAi) targeting CSFV viral genes were mentioned and the possible mechanisms related to anti-CSFV effects were also summarized.

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Prostaglandin A1 Inhibits Replication of Classical Swine Fever Virus

Cited by 4 publications

References 14 publications

Prostaglandin A1 triggers Mayaro virus inhibition and heat shock protein 70 expression in an epithelial cell model

Prostaglandin A1 triggers Mayaro virus inhibition and heat shock protein 70 expression in an epithelial cell model

Prostaglandin A1 inhibits the replication of bovine viral diarrhea virus

Anti-Classical Swine Fever Virus Strategies

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