Prostaglandin A1 inhibits the replication of bovine viral diarrhea virus

Caldas, Lúcio Ayres; Freitas, Tânia Rosária Pereira; Azevedo, Renata Campos; Souza, Wanderley de

doi:10.1016/j.bjm.2017.12.010

Cited by 6 publications

(5 citation statements)

References 16 publications

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“…Nevertheless, we could not unambiguously identify viral particles in placentae from COVID-19 pregnant women. Drastic cytoplasm vacuolization that we reported in SARS-CoV-2 trophoblast cells was previously described in an in vitro model of human conducting airway epithelium and Madin-Darby bovine kidney cells for SARS-CoV [51,52] leading to the hypothesis that vacuoles, in terms of early and late endosomes, could have a key role in the virus assembly process [53]. Accordingly, it was recently proposed that the downregulation of Rab7 small GTPase protein found in placentae from COVID-19 pregnant women could result in retention of the virus in the early endosomes or trapping within late endosomes and MVB, mediating physiological placental blockade of SARS-CoV-2 in pregnancy and consequently its vertical transmission.…”

Section: Discussionsupporting

confidence: 66%

Increased Placental Anti-Oxidant Response in Asymptomatic and Symptomatic COVID-19 Third-Trimester Pregnancies

et al. 2022

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Despite Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) -induced Oxidative Stress (OxS) being well documented in different organs, the molecular pathways underlying placental OxS in late-pregnancy women with SARS-COV-2 infection are poorly understood. Herein, we performed an observational study to determine whether placentae of women testing positive for SARS-CoV-2 during the third trimester of pregnancy showed redox-related alterations involving Catalase (CAT) and Superoxide Dismutase (SOD) antioxidant enzymes as well as placenta morphological anomalies relative to a cohort of healthy pregnant women. Next, we evaluated if placental redox-related alterations and mitochondria pathological changes were correlated with the presence of maternal symptoms. We observed ultrastructural alterations of placental mitochondria accompanied by increased levels of oxidative stress markers Thiobarbituric Acid Reactive Substances (TBARS) and Hypoxia Inducible Factor-1 α (HIF-1α) in SARS-CoV-2 women during the third trimester of pregnancy. Importantly, we found an increase in placental CAT and SOD antioxidant enzymes accompanied by physiological neonatal outcomes. Our findings strongly suggest a placenta-mediated OxS inhibition in response to SARS-CoV-2 infection, thus contrasting the cytotoxic profile caused by Coronavirus Disease 2019 (COVID-19).

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Section: Discussionsupporting

confidence: 66%

Increased Placental Anti-Oxidant Response in Asymptomatic and Symptomatic COVID-19 Third-Trimester Pregnancies

et al. 2022

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“…3A). Drastic vacuolization due to viral infection was previously described for other RNA viruses including SARS-CoV [20,31]. Similar sites were recently reported as virus morphogenesis matrix vesicae (VMMV) [19].…”

Section: Vacuoles Containing Viral Particlessupporting

confidence: 72%

Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron microscopy

Caldas¹,

Carneiro²,

Higa

et al. 2020

Preprint

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HighlightsWe used high resolution scanning electron microscopy to investigate Vero cells infected with SARS-CoV-2 at 2 and 48 hours post-infection. The central conclusions of this work include:• Infected cells display polarization of their cytosol forming a restricted viroplasm-like zone dedicated to virus production and morphogenesis.• This is the first demonstration of SARS-CoV-2 attachment by scanning electron microscopy.• This is the first scanning electron microscopy images of the interior of SARS-CoV-2 infected cells and exploration of their vacuole contents.• Perspective-viewing of bordering vesicles in close association with vacuoles.• Observation of membrane ruffles and structures suggestive of exocytosis on the surface of infected cells.• The first demonstration of viral surfing in cell-to-cell communication on SARS-CoV-2 infection.Abstract SARS-CoV-2 is the cause of the ongoing COVID-19 pandemic. Here, we investigated the interaction of this new coronavirus with Vero cells using high resolution scanning electron microscopy. Surface morphology, the interior of infected cells and the distribution of viral particles in both environments were observed 2 and 48 hours after infection. We showed areas of viral processing, details of vacuole contents, and viral interactions with the cell surface. Intercellular connections were also approached, and viral particles were adhered to these extensions suggesting direct cell-to-cell transmission of SARS-CoV-2.

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“…3 A). Drastic vacuolization due to viral infection was previously described for other RNA viruses including SARS-CoV 20 , 32 . Similar sites were recently reported as virus morphogenesis matrix vesicae (VMMV) 19 .…”

Section: Discussionmentioning

confidence: 72%

Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron microscopy

Caldas

Carneiro

Higa

et al. 2020

Sci Rep

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SARS-CoV-2 is the cause of the ongoing COVID-19 pandemic. Here, we investigated the interaction of this new coronavirus with Vero cells using high resolution scanning electron microscopy. Surface morphology, the interior of infected cells and the distribution of viral particles in both environments were observed 2 and 48 h after infection. We showed areas of viral processing, details of vacuole contents, and viral interactions with the cell surface. Intercellular connections were also approached, and viral particles were adhered to these extensions suggesting direct cell-to-cell transmission of SARS-CoV-2.

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Prostaglandin A1 inhibits the replication of bovine viral diarrhea virus

Cited by 6 publications

References 16 publications

Increased Placental Anti-Oxidant Response in Asymptomatic and Symptomatic COVID-19 Third-Trimester Pregnancies

Increased Placental Anti-Oxidant Response in Asymptomatic and Symptomatic COVID-19 Third-Trimester Pregnancies

Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron microscopy

Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron microscopy

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