Vaccine strategies against schistosomiasis

Capron, A.; Dessaint, J. P.; Capron, M.; Pierce, Raymond J.

doi:10.1590/s0074-02761992000800003

Cited by 15 publications

(14 citation statements)

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“…Extensive work has been carried out to identify schistosome molecules that confer partial but significant protection in different animal models. These include the Schistosoma mansoni 28-kDa and the S. japonicum 26-kDa glutathione S-transferase (GST) (5,32), the S. mansoni and S. japonicum 97-kDa paramyosin (13,25), the S. mansoni 28-kDa triose phosphate isomerase (29), the S. mansoni 23-kDa integral membrane antigen (24), and so forth. These vaccine candidates were selected by the World Health Organization for a series of independent trials to test their protective efficacy in laboratory animals (2).…”

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confidence: 99%

Vaccination with Calpain Induces a Th1-Biased Protective Immune Response againstSchistosoma japonicum

et al. 2001

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A large subunit of calpain, a calcium-activated neutral proteinase, from Schistosoma japonicum was cloned and expressed in Escherichia coli. When BALB/c mice were immunized with purified recombinant calpain (r-calpain) emulsified in complete Freund's adjuvant, a significant reduction in the number of recovered worms and also in egg production per female worm was observed (P < 0.01). Spleen cells of the immunized mice showed enhanced production of gamma interferon (IFN-␥) by activated CD4 ؉ T cells. Considering our observation of elevated expression of inducible nitric oxide synthase mRNA in immunized mice, r-calpaininduced IFN-␥ seemed to upregulate the production of nitric oxide by macrophages and subsequently mediated the killing of schistosomulae in the lung. On the other hand, spleen cells of immunized mice showed only faint interleukin-4 production in response to r-calpain in vitro, suggesting that immunization with r-calpain alters the Th1-Th2 balance in murine hosts even during a Th2-promoting S. japonicum infection. Furthermore, histopathological study of the livers of immunized mice showed that granulomas formed around eggs were diminished in both size and number. Egg production by female worms was clearly decreased in immunized mice, suggesting that r-calpain also has antifecundity effects. Taken together, these results point to S. japonicum calpain as a potential vaccine candidate for both worm killing and disease prevention, possibly through the induction of a strong Th1-dominant environment in immunized mice.More than 200 million people have schistosomiasis, and almost 600 million people are exposed to the risk of schistosomiasis (36). Chemotherapy is currently the choice for control; however, vaccine development remains an important long-term goal for the integrated control of schistosomiasis because of high reinfection rates in areas where the disease is endemic. Extensive work has been carried out to identify schistosome molecules that confer partial but significant protection in different animal models. These include the Schistosoma mansoni 28-kDa and the S. japonicum 26-kDa glutathione S-transferase (GST) (5, 32), the S. mansoni and S. japonicum 97-kDa paramyosin (13, 25), the S. mansoni 28-kDa triose phosphate isomerase (29), the S. mansoni 23-kDa integral membrane antigen (24), and so forth. These vaccine candidates were selected by the World

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confidence: 99%

Vaccination with Calpain Induces a Th1-Biased Protective Immune Response againstSchistosoma japonicum

et al. 2001

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“…The list of antigens and associated prophylactic efficacy has been extensively reviewed in the last few years. 10,12,16,27,[42][43][44][45] From all of these antigens, only one S. hematobium antigen, Sh-28-GST, has advanced into clinical trials, though this occurred over a decade ago and detailed findings are not available. 46 Another important schistosome antigen, Sm-14, a fatty acid binding protein has also been developed for clinical trials.…”

Section: Vaccine Candidates and Search Of Functionally Important Vaccmentioning

confidence: 99%

Schistosomiasis vaccines

Siddiqui

Ganley-Leal

2011

Human Vaccines

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“…However, in order to attain the goal of filariasis elimination, it is desirable to have more than one control tool. Recently, great deal of interest has been generated in the possibility of blocking the development and transmission of parasites by vectors of diseases such as leishmaniasis (Tonui 1999), schistosomiasis (Capron et al 1992), and Lyme disease (de Silva et al1996). In the case of malaria, transmission-blocking vaccines have reached advanced stage of development (Kaslow 1997, Carter 2001.…”

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“…Vector transmission-blocking antibodies have been reported with respect to other parasites causing malaria (Khurna & Talib 1996, Carter 2001, leishmaniasis (Tonui 1999), schistosomiasis (Capron et al 1992), and Lyme disease (de Silva et al 1996). With respect to filarial parasites, a monoclonal antibody, MF1, has been reported to degrade the chitin containing structures in the microfilaria or in its mosquito vector during B. malayi development and transmission (Fuhrman et al1992).…”

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Suppression of Brugia malayi (sub-periodic) larval development in Aedes aegypti (Liverpool strain) fed on blood of animals immunized with microfilariae

Mary

Paily

Hoti

2005

Mem. Inst. Oswaldo Cruz

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(Ottesen et al. 1997). However, in order to attain the goal of filariasis elimination, it is desirable to have more than one control tool. Recently, great deal of interest has been generated in the possibility of blocking the development and transmission of parasites by vectors of diseases such as leishmaniasis (Tonui 1999), schistosomiasis (Capron et al. 1992), and Lyme disease (de Silva et al.1996). In the case of malaria, transmission-blocking vaccines have reached advanced stage of development (Kaslow 1997, Carter 2001. But there is only one such study on the antibodies regulating the development of filarial parasites (Fuhrman et al. 1992), which reports that a monoclonal antibody, MF1, could degrade the chitin containing structures of B. malayi mf in the vector mosquito. In the present paper, results of a preliminary study on the role of anti-filarial polyclonal antibodies in suppressing the development of early larval stages of the parasite in the mosquito host are presented.Mongolian gerbils, Meriones unguiculatus, were immunized by intra-peritoneal (i/p) inoculation of microfilariae (mf) of B. malayi (sub-periodic strain). obtained by lavaging the peritoneal cavity of B. malayi infected Mongolian gerbils. The animals were 6-8 weeks old and the mf were harvested on 120 days post-inoculation of infective larvae to ensure that the mf were identical with respect to their age and viability. The harvested mf were purified by Percoll gradient centrifugation (Chandrashekar et al.1984), and were used for immunization as well as feeding the mosquitoes. Either single dose of 1000 live mf or their homogenate (20 µg of protein) per animal was given in sterile phosphate buffered saline (PBS, pH 7.4). Homogenate of mf was prepared in PBS containing a cock-tail of protease inhibitors (Maizles et al. 1999) such as, EDTA-1M, EGTA-1M, NEM-1M, Pepstatin-1 mM, PMSF-0.33 Mm, and TPCK-0.1 M at 5 µl level/ml of the parasite extract. The animals were immunized in duplicate for each antigen type and an equal numbers were left as controls with only PBS inoculation. Considering the ethical issues involved in the usage of laboratory animals and the duration involved, the number of animals used in this experiment was restricted to only two in each group. The antibody titre in each animal was determined two weeks after immunization by direct enzyme linked immunosorbent assay (ELISA) as per Voller et al. (1976), using mf homogenate antigen. Briefly, crude antigen of B. malayi was coated (1 µg/well) onto 96 well polysterene plates (Nunc-US) and incubated overnight at 4 o C. Sera samples from the experimental and control animals diluted to 1:100 with 0.75% bovine serum albumin (BSA) in PBS/ T were reacted for 2 h after blocking the wells with 1% BSA in PBS/T. Anti-mouse peroxidase conjugate (Sigma Chem. Co., US) was allowed to react with the antigenantibody complex, followed by the addition of θ-Phenylenediamine dihydrochloride (Sigma Chem. Co., US) as substrate in phosphate citrate buffer (pH 5.0). The reac- tion was arrested with 5 N ...

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Vaccine strategies against schistosomiasis

Cited by 15 publications

References 0 publications

Vaccination with Calpain Induces a Th1-Biased Protective Immune Response againstSchistosoma japonicum

Vaccination with Calpain Induces a Th1-Biased Protective Immune Response againstSchistosoma japonicum

Schistosomiasis vaccines

Suppression of Brugia malayi (sub-periodic) larval development in Aedes aegypti (Liverpool strain) fed on blood of animals immunized with microfilariae

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