2001
DOI: 10.1128/iai.69.1.386-391.2001
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Abstract: A large subunit of calpain, a calcium-activated neutral proteinase, from Schistosoma japonicum was cloned and expressed in Escherichia coli. When BALB/c mice were immunized with purified recombinant calpain (r-calpain) emulsified in complete Freund's adjuvant, a significant reduction in the number of recovered worms and also in egg production per female worm was observed (P < 0.01). Spleen cells of the immunized mice showed enhanced production of gamma interferon (IFN-␥) by activated CD4 ؉ T cells. Considering… Show more

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Cited by 55 publications
(43 citation statements)
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References 37 publications
(22 reference statements)
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“…Our findings complement an earlier report in which a CD4 ϩ Th1 clone recognizing Sm-p80 was found to be capable of inducing protection of mice against cercarial challenge (28). Furthermore, a homolog of calpain was also found to induce a Th1-biased protective immune response against Schistosoma japonicum (61,62). Furthermore, IgG3 titers were increased in the Sm-p80-pcDNA3 ϩ pORF-mIL-4 group, but the increase did not lead to any statistically significant increase in the levels of protection.…”
Section: Discussionmentioning
confidence: 96%
“…Our findings complement an earlier report in which a CD4 ϩ Th1 clone recognizing Sm-p80 was found to be capable of inducing protection of mice against cercarial challenge (28). Furthermore, a homolog of calpain was also found to induce a Th1-biased protective immune response against Schistosoma japonicum (61,62). Furthermore, IgG3 titers were increased in the Sm-p80-pcDNA3 ϩ pORF-mIL-4 group, but the increase did not lead to any statistically significant increase in the levels of protection.…”
Section: Discussionmentioning
confidence: 96%
“…Wild-type C57BL/6 mice purchased from JAPAN SLC. Inc. at the age of 8 weeks were infected percutaneously with the 20 -25 cercariae obtained from O. nosophora (20). Establishment of the infection was confirmed by measuring the antisoluble egg antigen titer at 4 and 7 weeks and the mice were sacrificed by cervical dislocation.…”
Section: Methodsmentioning
confidence: 99%
“…S. japonicum was maintained by passage through Oncomelania nosophora and BALB/C mice (20). Wild-type C57BL/6 mice purchased from JAPAN SLC.…”
Section: Methodsmentioning
confidence: 99%
“…Based on these data, it is our assessment that Sm-p80, in a recombinant protein formulation, is now ready to be moved into the next phase, i.e., scale up and cGMP production in preparation for Phase I/II human clinical trials. Overall, we believe that calpain has a great potential as an important vaccine antigen for the reduction of the morbidity associated with both S. mansoni and S. japonicum 63,64,70 infections. The immunobiological goals of a Sm-p80-based vaccine would be blocking an essential parasite function via IgG.…”
Section: O N O T D I S T R I B U T Ementioning
confidence: 90%