Fixação de complemento na blastomicose

Moses, Arthur

doi:10.1590/s0074-02761916000200006

Cited by 18 publications

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“…In 1916, Moses [1] was the first to use P. brasiliensis antigens for serologic testing, using a saline extract from the fungus, for successful testing in CF. Since this study, a number of different techniques for the use and preparation of antigens have been described.…”

Section: Antigen Preparationmentioning

confidence: 99%

Serology of paracoccidioidomycosis

Camargo

2008

Mycopathologia

114

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This review provides the background for understanding the role of a battery of diagnostic methods in paracoccidioidomycosis (PCM). This systemic mycosis is a disease endemic in many regions of Latin America, with sporadic cases also occurring throughout the world (mycosis of importation). Although excellent laboratory methods for diagnosis are available, there are deficiencies that must be met by continued research. Understanding the uses and limitations of a battery of laboratory methods is essential to diagnose PCM. Clinicians and laboratory directors must be familiar with the uses and limitations of a battery of serologic and mycological tests to accurately diagnose of PCM. Antibody and antigen detections are valuable adjuncts to histopathology and culture. More recently, the gp43 and gp70 antigen detection assay have improved the methodology of diagnosis of this mycosis, which improves reproducibility and facilitates monitoring antigen clearance during antifungal treatment. Furthermore, detection of antigen in cerebrospinal fluid and in bronchoalveolar lavage fluid increases the sensitivity for diagnosis of PCM in central nervous system and in pulmonary infections, respectively.

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Section: Antigen Preparationmentioning

confidence: 99%

Serology of paracoccidioidomycosis

Camargo

2008

Mycopathologia

114

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“…Assessment of the serological response has long been used in PCM with two main purposes. Since its introduction by Moses in 1916 [3], it has been used mainly as a diagnostic tool, because in a significant proportion of the patients it is difficult to obtain clinical specimens to visualise or cultivate the fungus, or both, because only deep-sited lesions are present. Also, it has been used to follow up patients under treatment.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of tests for antibody response in the follow-up of patients with acute and chronic forms of paracoccidioidomycosis

Negro¹,

Pereira²,

Andrade

et al. 2000

Journal of Medical Microbiology

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Several serological tests have been used successfully in the diagnosis of paracoccidioidomycosis (PCM). In contrast, data about the use of these tests in the follow-up of PCM patients have been heterogeneous. In this study, serum samples from 43 PCM patients with different clinical forms were analysed by counter-immuno-electrophoresis (CIE), complement fixation (CF) and ELISA before treatment. With CIE and ELISA, the chronic unifocal form showed significantly lower antibody levels compared with chronic multifocal and acute forms. Acute form patients had significantly higher titres than patients with multifocal disease by CIE but not by ELISA. No significant differences were observed with CF. Twenty-seven of these patients were followed-up for 2 years and showed a decline in antibody levels by all three tests, paralleling clinical improvement. However, only patients with unifocal disease cleared their antibodies after 1 year of treatment as analysed by CF and ELISA and after 2 years by CIE, suggesting that these patients may need shorter courses of therapy. Patients with the other clinical form of the disease needed > or =2 years of therapy to clear their antibodies. Sera from a further five patients who presented with a relapse were analysed. At the time of relapse all showed increases in antibody levels by CIE and ELISA, but only three showed increases by CF tests. Therefore, CIE and ELISA demonstrated a better clinical correlation than CF, probably reflecting the fungal burden of PCM patients more accurately.

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“…Several serological tests for detection of anti-P. brasiliensis antibodies, such as complement fixation (14), double immunodiffusion (DID) (6), counterimmunoelectrophoresis (8), the enzyme-linked immunosorbent assay (ELISA) (11), and dot blot analysis (11,21), can be used in the diagnosis of PCM. However, none of these has the characteristics of high sensitivity and specificity needed for an accurate diagnosis (1,8,10).…”

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confidence: 99%

Serological Diagnosis of Paracoccidioidomycosis through a Western Blot Technique

Perenha-Viana

Gonzales

Brockelt

et al. 2012

Clin. Vaccine Immunol.

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ABSTRACTParacoccidioidomycosis (PCM) is a serious infectious disease that progresses toward death if untreated. Its confirmatory diagnosis is made by the detection of the fungusParacoccidioides brasiliensisin a direct mycological examination or by histopathology. However, these techniques are of low sensitivity. Serological tests seem to be more promising. The objective of this study was to test Western blot (WB) analysis using sera from patients suspected of PCM to determine whether it represents a safe and sensitive serological technique for a rapid and effective diagnosis for this disease. Sera from 517 patients were analyzed through WB analysis and double-immunodiffusion (DID) techniques using a crude exoantigen ofP. brasiliensis339. DID gave positive reactions for 140 sera (27%) and WB for 250 sera (48.4%). All sera that had a positive reaction by DID also had a positive result with a 43-kDa glycoprotein by WB analysis. Among the 377 samples that were negative by DID, 29.1% were reactive in WB analysis. For the cutoff dilution used (1:400), a positive reaction was not observed with any of the 102 sera from patients with other diseases in regions where such diseases are endemic and 30 healthy individuals tested as negative controls. These results prove WB analysis to be a sensitive technique and suggest its inclusion among routine laboratory assays as a safe method for PCM diagnosis.

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Fixação de complemento na blastomicose

Cited by 18 publications

References 0 publications

Serology of paracoccidioidomycosis

Serology of paracoccidioidomycosis

Evaluation of tests for antibody response in the follow-up of patients with acute and chronic forms of paracoccidioidomycosis

Serological Diagnosis of Paracoccidioidomycosis through a Western Blot Technique

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