Evaluation of tests for antibody response in the follow-up of patients with acute and chronic forms of paracoccidioidomycosis

Negro, G. Del; Pereira, Cristiane N.; Andrade, Heitor Franco de; Palácios, Selma A.; Vidal, Mônica M. S.; Charbel, Cecília Eugênia; Benard, Gil

doi:10.1099/0022-1317-49-1-37

Cited by 50 publications

(55 citation statements)

References 37 publications

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“…Falsenegative results for specific antibodies may occur in patients not infected with HIV, especially those with the chronic form of the disease and an unifocal, small lesion (Del Negro et al 2000). Patients with this form of paracoccidioidomycosis have a less intense humoral immunological response than patients with acute/subacute form of the disease (Panunto-Castelo et al 2003).…”

Section: Discussionmentioning

confidence: 99%

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Serological diagnosis of paracoccidioidomycosis in HIV-coinfected patients

Bellissimo-Rodrigues¹,

Vitali²,

Martínez

2010

Mem. Inst. Oswaldo Cruz

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Paracoccidioidomycosis should be differentiated from other opportunistic diseases in human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) patients who live in Latin America. Laboratory investigation can begin with serological tests, which are rapid and efficient. In the present study, double immunodiffusion (DID), counterimmunoelectrophoresis (CIEP) and an enzyme linked immunosorbent assay (ELISA) tests were assessed for the detection of anti-Paracoccidioides brasiliensis antibodies in 40 patients coinfected with HIV. The results were compared to those obtained for 75 non-HIV-infected patients with endemic paracoccidioidomycosis. Anti-P. brasiliensis antibodies were detected in 65% (DID), 79% (CIEP) and 95% (ELISA) of the patients with HIV/AIDS, significantly lower rates than those detected in cases of endemic paracoccidioidomycosis, which were 89%, 99% and 100%, respectively. The reactive sera of HIV-infected patients also showed lower anti-P. brasiliensis antibody titres than those of non-HIV-infected patients. Despite the lower intensity of the specific humoral response, serological tests are useful for the diagnosis of opportunistic paracoccidioidomycosis in the HIV/AIDS population. We suggest optimization of the laboratory diagnosis by combining the ELISA test with CIEP or DID

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Section: Discussionmentioning

confidence: 99%

“…The titration of anti-P. brasiliensis antibodies is important for the laboratory diagnosis and follow up of paracoccidioidomycosis. Patients with active disease usually have high antibody levels, which decline with antifungal therapy and clinical recovery (Del Negro et al 2000).…”

mentioning

confidence: 99%

Serological diagnosis of paracoccidioidomycosis in HIV-coinfected patients

Bellissimo-Rodrigues¹,

Vitali²,

Martínez

2010

Mem. Inst. Oswaldo Cruz

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“…Os testes ELISA e imunodifusão foram úteis para detectar recaída da doença, conforme já assinalada a importância da associação do ELISA com outro teste sorológico 11 . Em geral, observamos títulos altos à admissão, e baixos ou negativos a partir de 12 o mês de tratamento.…”

Section: Discussionunclassified

Aspectos laboratoriais evolutivos de crianças em tratamento da paracoccidioidomicose

Nogueira

Andrade

Tonelli

et al. 2006

Rev. Soc. Bras. Med. Trop.

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RESUMOEstudo de 38 crianças com paracoccidioidomicose, até 14 anos de idade, tratados por 24-30 meses, com um derivado sulfamídico ou cetoconazol, desde o início do tratamento ou em seguida ao uso da anfotericina B. Dados laboratoriais à admissão foram analisados e comparados com exames seqüenciais até 30 meses de acompanhamento. Anemia, eosinofilia e bilirrubinas e aminotransferases elevadas normalizaram-se, na maioria, até os três meses de tratamento e, hipoalbuminemia, até os seis meses, sugerindo que esses achados sejam bons parâmetros para o monitoramento da resposta terapêutica inicial. Leucócitos periféricos, velocidade de hemossedimentação, IgG e títulos sorológicos para o Paracoccidioides brasiliensis estavam elevados e normalizaram-se, freqüentemente, após nove a 12 meses de tratamento, o que sugere utilidade desses exames no monitoramento de todo o tratamento e enfatiza a necessidade de curso terapêutico prolongado da paracoccidioidomicose na infância. Palavras-chaves: Paracoccidioidomicose. Criança. Seguimento de exames laboratoriais. ABSTRACTThe study of 38 children with paracoccidioidomycosis, aged up to 14, treated for 24 to 30 months with either a sulfonamide derivative or ketoconazole either alone or, after the use of amphotericin B. Laboratory data at admission were analyzed and compared with those of sequential tests after up to 30 months follow-up. Anemia, eosinophilia, increased bilirubin and aminotransferases normalized, in most patients, after three months treatment and hypoalbuminemia normalized after six months, suggesting that these laboratory findings are useful for monitoring early therapeutic response. Peripheral leucocytes, erythrocyte sedimentation rate, IgG, and serological titers for Paracoccidioides brasiliensis were increased and frequently normalized after nine to 12 months of treatment. They may be useful for monitoring the entire treatment and emphasize the need for long term treatment of paracoccidioidomycosis in children. Key-words: Paracoccidioidomycosis. Children. Follow-up laboratory.A paracoccidioidomicose (PCM) é uma micose sistêmica que ocorre caracteristicamente em regiões rurais da América Latina, sendo o Brasil o país com o maior número de casos da doença. Afeta pessoas previamente hígidas, tem curso crônico e, muitas vezes, com gravidade. Informações sobre a evolução das alterações laboratoriais de pacientes em tratamento ainda são escassas e resultam, na maioria, de casuísticas pequenas e tempo de acompanhamento inferior ao tempo de tratamento. É conhecido o valor de exames como a sorologia e a velocidade de hemossedimentação no monitoramento terapêutico dos pacientes 1 8 11 27 . Entretanto, apesar dos avançados estudos no campo da imunologia e do

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“…Serological tests are useful, and IgG, IgM, and IgE are regularly detected, but sensitivity is reduced in patients with disorders that cause immunosuppression (100,109). The ID test is more specific than CF but is not quantitative.…”

Section: Other Regional Fungal Infectionsmentioning

confidence: 99%

Transmission of Tropical and Geographically Restricted Infections during Solid-Organ Transplantation

et al. 2008

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SUMMARY In recent years, the increasing number of donors from different regions of the world is providing a new challenge for the management and selection of suitable donors. This is a worldwide problem in most countries with transplantation programs, especially due to the increase in immigration and international travel. This paper elaborates recommendations regarding the selection criteria for donors from foreign countries who could potentially transmit tropical or geographically restricted infections to solid-organ transplant recipients. For this purpose, an extensive review of the medical literature focusing on viral, fungal, and parasitic infections that could be transmitted during transplantation from donors who have lived or traveled in countries where these infections are endemic has been performed, with special emphasis on tropical and imported infections. The review also includes cases described in the literature as well as risks of transmission during transplantation, microbiological tests available, and recommendations for each infection. A table listing different infectious agents with their geographic distributions and specific recommendations is included.

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Evaluation of tests for antibody response in the follow-up of patients with acute and chronic forms of paracoccidioidomycosis

Cited by 50 publications

References 37 publications

Serological diagnosis of paracoccidioidomycosis in HIV-coinfected patients

Serological diagnosis of paracoccidioidomycosis in HIV-coinfected patients

Aspectos laboratoriais evolutivos de crianças em tratamento da paracoccidioidomicose

Transmission of Tropical and Geographically Restricted Infections during Solid-Organ Transplantation

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