Serological Diagnosis of Paracoccidioidomycosis through a Western Blot Technique

Perenha-Viana, Maysa Cláudia Zolin; Gonzales, Isabella Assis Alavarse; Brockelt, Sonia Regina; Machado, Luzia Néri; Svidzinski, T. I. E.

doi:10.1128/cvi.05693-11

Cited by 26 publications

(22 citation statements)

References 21 publications

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“…On the other hand, WB allowed demonstration of antibodies to the specific antigen (gp43) in sera of 100% of patients positive for PCM. These antibodies have been considered important markers for the diagnosis of PCM (3, 6,12,16).The major contribution of this preliminary study was, therefore, to show that the WB technique can be used as a tool to confirm the differential diagnosis between TB and PCM and to reinforce our previous publication (12), and WB proved to be a particularly useful tool for PCM patients with a negative DID test. The use of this technique resulted in referrals for appropriate treatment of at least six patients who were triaged and referred for AFB investigation.…”

supporting

confidence: 58%

mentioning

confidence: 99%

“…On the other hand, more-sensitive tests such as enzyme-linked immunosorbent assay (ELISA) have shown cross-reactivity (9,17), including that with tuberculosis (10,14). Recently, we showed that Western blotting (WB) could contribute to the diagnosis of PCM, delivering safe, reliable, and fast results (12). Now, our objective was to apply WB to patients suspected of TB, in order to investigate PCM also.…”

mentioning

confidence: 99%

“…Samples of sputum and serum from 134 human patients with pulmonary symptoms, who were selected from a research project that investigates PCM and TB simultaneously, were analyzed to perform the complete laboratory battery of the tests, including bacilloscopy and AFB culture, DME, and ELISA (8) and DID testing and WB (12). Sera from patients with TB or PCM exclusively (confirmed by clinical and laboratory criteria) were used as controls.…”

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confidence: 99%

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Western Blotting Is an Efficient Tool for Differential Diagnosis of Paracoccidioidomycosis and Pulmonary Tuberculosis

Bertoni

Perenha-Viana

Patussi

et al. 2012

Clin. Vaccine Immunol.

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b Sputum and sera from 134 patients screened for tuberculosis (TB) were analyzed to investigate TB and paracoccidioidomycosis (PCM). Of these patients, 11 (8.2%) were confirmed to have TB, but six (4.5%) were positive only for PCM. All patients with PCM presented anti-43-kDa-component antibodies in Western blotting (WB) assays, while in the TB-positive patients these antibodies did not appear. This preliminary study suggests WB as a potential tool for differential laboratory diagnosis between TB and PCM. P aracoccidioidomycosis (PCM) is a mycosis endemic to Latin America caused by Paracoccidioides brasiliensis and also by the recently described Paracoccidioides lutzii. It is an important systemic mycosis, which presents with a wide range of clinical signs and symptoms. Although PCM has been described for more than 100 years and is considered endemic in many countries, until now there have been serious problems in relation to differential diagnosis of this important systemic mycosis (11, 13). The lungs are affected in about 75% of cases, and the initial pulmonary lesions are similar to those of tuberculosis (TB) (7). Furthermore, the association between PCM and TB is not uncommon; it occurs at a frequency varying between 5.5 and 15.8% (10, 13), and so differential diagnosis between these two diseases as well as detection of coinfection with TB and PCM is very important.A characterization based only on clinical and radiological data can be difficult, especially in areas of endemicity, since the two diseases may occur simultaneously or sequentially. Diagnostic error can occur, especially in basic health units, as a consequence of the fact that the clinical history and radiological findings do not always allow a clear distinction between the two diseases (13). This is a serious public health problem, since incorrect treatment increases the risk of pulmonary sequelae such as fibrosis, bronchiectasis, and chronic respiratory insufficiency.The definitive diagnosis of PCM has been established by the finding of budding yeast cells of P. brasiliensis through direct mycological examination (DME) of fresh biological material such as sputum, by histopathological techniques, or, alternatively, by isolation and identification of the fungus in culture (15). Similarly, the diagnosis of TB is established by bacilloscopy, a direct investigation of the acid-fast bacilli (AFB), and by isolation and identification of Mycobacterium tuberculosis. However, these techniques have some important limitations that are inherent in the nature of each one: the low sensitivity of the direct techniques (DME and bacilloscopy) and the long time necessary for development and identification of the agents are the most common problems. Furthermore, the difficulty in obtaining the most appropriate samples of biological material means that sputum is routinely used to investigate AFB and P. brasiliensis. However, spontaneous or induced sputum is highly contaminated and may carry only a small number of pathogenic microorganisms, insufficient to provide a p...

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supporting

confidence: 58%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Western Blotting Is an Efficient Tool for Differential Diagnosis of Paracoccidioidomycosis and Pulmonary Tuberculosis

Bertoni

Perenha-Viana

Patussi

et al. 2012

Clin. Vaccine Immunol.

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“…Portanto, a identificação e caracterização das mesmas podem contribuir para o entendimento dos mecanismos de patogenicidade nos casos da conidiobolomicose ovina, bem como na utilização para diagnósticos sorológicos ou como candidatos a vacinas. Estudos conduzidos em fungos patogênicos de importância biomédica já permitiram obter importantes informações sobre proteínas imunogênicas com papel fundamental no processo de invasão, sobrevivência no hospedeiro e como possíveis candidatos a vacina (Camargo et al 1989, Krajaejun et al 2006, Chindamporn et al 2009, Perenha-Viana et al 2012, Magalhães et al 2012.…”

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Proteínas imunorreativas de Conidiobolus lamprauges isoladas de ovinos infectados naturalmente

et al. 2015

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O estudo de conidiobolomicose ovina tem sido realizado nos seus aspectos clínicos, epidemiológicos, patológicos e moleculares. Informações, entretanto, sobre a resposta imune do hospedeiro na infecção por Conidiobolus lampraugessão inexistentes. Este estudo teve por objetivo a identificação de proteínas imunorreativas que possam desempenhar papel importante na resposta imune de ovinos naturalmente infectados por C. lamprauges. Para a caracterização protéica e imunológica foi utilizada a cepa de C. lamprauges(FIOCRUZ-INCQS 40316) isolada de ovino com sinais clínicos de conidiobolomicose no Estado do MT e cinco amostras de soro de ovinos infectados naturalmente pelo fungo. A presença de anticorpos IgG foi observada em todos os animais doentes com títulos reagentes em diluições de até 1:1.600. Na técnica do immunoblot, o perfil antigênico frente aos soros ovinos com a doença apresentou doze bandas reativas, com massas moleculares variando de 35 a 198 kDa. Dentre estas, a proteína de 198 kDa foi reativa em 3 soros de ovinos e a de 53 kDa apresentou a maior intensidade comparativamente com outras bandas, sendo provavelmente imunodominante. Amostras de soro de animais sadios não apresentaram reatividade demostrando a especificidade da técnica. A presença de proteínas antigênicas de C. lamprauges e IgG específicos em soros de ovinos observados no presente trabalho poderá auxiliar no desenvolvimento de métodos de diagnóstico precoces e na utilização de proteínas candidatas a vacinas para o controle e prevenção da infecção em animais e humanos.

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Applications of western blot technique: From bench to bedside

Meftahi

Bahari

Mahmoudabadi

et al. 2021

Biochem Molecular Bio Educ

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Western blot (WB) or immunoblot is a workhorse method. It is commonly used by biologists for study of different aspects of protein biomolecules. In addition, it has been widely used in disease diagnosis. Despite some limitations such as long time, different applications of WB have not been limited. In the present review, we have summarized scientific and clinical applications of WB. In addition, we described some new generation of WB techniques.

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Serological Diagnosis of Paracoccidioidomycosis through a Western Blot Technique

Cited by 26 publications

References 21 publications

Western Blotting Is an Efficient Tool for Differential Diagnosis of Paracoccidioidomycosis and Pulmonary Tuberculosis

Western Blotting Is an Efficient Tool for Differential Diagnosis of Paracoccidioidomycosis and Pulmonary Tuberculosis

Proteínas imunorreativas de Conidiobolus lamprauges isoladas de ovinos infectados naturalmente

Applications of western blot technique: From bench to bedside

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