Dos metodos biolojicos de diagnostico nas cisticercozes

Moses, Arthur

doi:10.1590/s0074-02761911000200008

Cited by 18 publications

(13 citation statements)

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“…1,2 The first serological assays for parasitic infections were complement precipitation and fixation techniques. In 1909, Weinberg used complement fixation with cystic fluid from cysticerci to demonstrate specific antibodies in the sera of a group of cysticercotic pigs.…”

Section: Antibody Detection Tests For Cysticercosismentioning

confidence: 99%

“…[4][5][6] In 1911, Arthur Moses reported the use of an aqueous cysticercal extract to demonstrate the presence of antibodies in the serum of three patients with subcutaneous cysticercosis and in the cerebrospinal fluid (CSF) of a patient with cysticercosis encephalitis, thus demonstrating for the first time, the presence of anti-cysticercal antibodies in CSF. 1,7 In the following decades, many attempts to develop better diagnostic tests focused on indirect, antibody detection assays. Antibody detection does not distinguish active from inactive infections, and is not useful to monitor changes over short periods; 8 however, its diagnostic efficacy is much higher than that of antigen detection assays.…”

Section: Antibody Detection Tests For Cysticercosismentioning

confidence: 99%

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Immunological and molecular diagnosis of cysticercosis

Rodríguez¹,

Wilkins

Dorny

2012

Pathogens and Global Health

133

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Cysticercosis, the infection with the larval stage of Taenia solium, is a cause of neurological symptoms including seizures, affecting the quality of life of patients and their families. Diagnosis focuses on brain imaging and serological tests are mostly used as confirmatory tools. Most cases, however, occur in poor endemic areas, where both kinds of diagnostic tools are poorly available. Development of point of care diagnostic tests is one of the most important priorities for cysticercosis researches today. The ideal point of care test would require detection of viable cysticercosis and hopefully identify cases with severe or progressive forms of neurocysticercosis, leading to referral of the patient for specialized medical attention. This manuscript describes the evolution of the serological diagnosis of cysticercosis over time, and the characteristics of the most common currently available tools, their advantages and disadvantages, and their potential use in future diagnostic tests.

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Section: Antibody Detection Tests For Cysticercosismentioning

confidence: 99%

Section: Antibody Detection Tests For Cysticercosismentioning

confidence: 99%

Immunological and molecular diagnosis of cysticercosis

Rodríguez¹,

Wilkins

Dorny

2012

Pathogens and Global Health

133

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“…First positive results in blood serum and in CSF were registered in Brazil in 1911 by Moses 6 . Researchers from several countries with high incidence of cysticercosis began to work on the subject since then.…”

Section: Commentsmentioning

confidence: 99%

Cysticercosis of the central nervous system and cerebrospinal fluid immunodiagnosis of 1573 patients in 63 years (1929-1992)

Spina-França

Livramento

Machado

1993

Arq. Neuro-Psiquiatr.

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SUMMARY -Attention given to prophylaxis of neurocysiticercosis (NC) is far beyond minimal needs among several regions of the in-development world, and for this reason incidence of the disease persists high among them. This investigation was carried out to show the extent of the problem by analysing the incidence of NC in a region of Brazil (São Paulo). CSF immunodiagnosis of NC by detecting antibodies to Cysticercus cellulosae in a neurodiagnostics laboratory is evaluated for this purpose. Cases studied in a 63-year period are reviewed. Total cases in this period is 139/000, and for 1,573 (1.13%) diagnosis is NC.Special characteristics were not detected for colour and sex prevalence. Age bracket prevalence is from 21 to 40 years old (55.3%) high rates occurring for women between 21 to 30 years old, and for men between 31 to 40.Cases distribution in five consecutive decades (1942-1991) shows no decreasing tendency: average incidence is 1% for the 50 years, and it is over this average for the last three decades. Data confirm that incidence continues expressively high throughout the 50 years covered by this study. Interruption of taenia/cysticercus cycle is the best way for reducing the incidence of cysticercosis of the central nervous system (neurocysticercosis), as recognized since longtime is. Like the mentioned, other prophylactic measures for fighting neurocysticercosis (NC) have not been satisfactorily adopted in many regions of the in-development world with high incidence of NC. As a consequence, incidence rates reported for NC persist high among them 1 2 , like in

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“…La réaction de fixation du complément (6,8,11) recherche les anticorps dans le sérum des malades, et le liquide céphalorachi dien où la réaction serait moins sensible (4,7,12). Les réactions de précipitation en milieu liquide sont utilisables malgré des difficultés dues à de fausses précipitations dues à la substance C (2, 3), avec quelques faux positifs chez des témoins normaux (10).…”

Section: Discussionunclassified

Apport de la technique de fluorescence indirecte au diagnostic immunologique de la cysticercose humaine

Dao

Arnaud

et al. 1973

Ann. Parasitol. Hum. Comp.

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RésuméLa technique d'immunofluorescence indirecte au cours de la cysticercose a été étudiée à l'aide de quatre antigènes différents : scolex, cysticerques, anneaux de T. saginata et T. solium. La sensibilité de la méthode est bonne, les taux varient dans les cas positifs du 1/40e au 1/640e. Les sérums positifs correspondent aux cysticercoses récentes, s'accompagnant de signes neurologiques notamment, et aux formes calcifiées généralisées encore évolutives. La sensibilité de l'immunofluorescence est supérieure à celle de l'immunoélectrophorèse.La spécificité de la méthode semble bonne, si l'on ne retient comme positifs que les sérums donnant une fluorescence au moins égale au 1/40e. Il n'y a pas de faux positifs, parmi les sérums témoins, si on emploie comme antigène une coupe d'anneau de T. saginata ou T. solium. Les quelques faux positifs ont tous été observés sur coupes de scolex d'E. granulosus, dont l'emploi pris isolément est donc déconseillé.Les réactions croisées ne permettent pas, quel que soit l'anti gène utilisé, de distinguer sérologiquement une cysticercose, un kyste hydatique, une échinococcose alvéolaire ou une cénurose. SummaryHuman cyticercosis studied by the indirect fluorescent techni que. -The authors have studied sera from 35 human cysticercosis cases, with the indirect fluorescent technique, using E. granulosus, C. cellulosae, T. saginata and T. solium as antigens.The sensibility of this method seems good, the titers of the sera varying in the positive cases from 1/40 to 1/640. The 13 positive cases correspond to recent infestations, particularly when neurologic symptoms are noted ; some disseminated calcified cysticercosis give sometimes high titers. Compared with the Immunoelectrophoresis, the indirect IF test is more sensitive.The specificity is quite good when T. solium and T. saginata are employed as antigens, if the minimum positive titer is 1/40 in the controlled sera. The specificity is not so good on slices of E. granulosus with the antihuman polyvalent gammaglobulin fluorescent rabbit serum. Anyway, if it is always possible to recognize sera from parasitoses with the 4 antigens employed, the cross reactions are very important between the Cestode group, and the IF test cannot separate coenurosis, echinococcosis and cysti cercosis. A more precise diagnosis needs then clinical, epidemio logical and histological data.

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Dos metodos biolojicos de diagnostico nas cisticercozes

Cited by 18 publications

References 0 publications

Immunological and molecular diagnosis of cysticercosis

Immunological and molecular diagnosis of cysticercosis

Cysticercosis of the central nervous system and cerebrospinal fluid immunodiagnosis of 1573 patients in 63 years (1929-1992)

Apport de la technique de fluorescence indirecte au diagnostic immunologique de la cysticercose humaine

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