Comparação de diferentes métodos para medida do tamanho do infarto experimental crônico em Ratos

Minicucci, Marcos F.; Azevedo, Paulo Furquim de; Duarte, Delfim; Matsubara, Beatriz Bojikian; Matsubara, Luiz S.; Campana, Álvaro Oscar; Paiva, Sérgio Alberto Rupp de; Zornoff, Leonardo A. M.

doi:10.1590/s0066-782x2007001400004

Cited by 11 publications

(5 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To evaluate the infarct size, the hearts were removed and dissected. Cardiac tissue samples were fixed in a 10 % formaldehyde solution for 48 h and stained with hematoxylin and eosin according to the previously described method [ 12 ]. The infarction size of left ventricle was determined in sections 5 to 6 mm from the apex, since the values in this region correspond to the mean of the values obtained from sections of the entire heart.…”

Section: Methodsmentioning

confidence: 99%

Gualou Xiebai decoction inhibits cardiac dysfunction and inflammation in cardiac fibrosis rats

Ding

Peng

Shen

et al. 2015

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundGualou Xiebai Decoction (GXD) is a well-known traditional Chinese recipe. It has been used to treat cardiovascular disorders for nearly two thousand years. But there is a lack of reports on cardiac fibrosis and underlying mechanism.MethodsMyocardial infarction was performed by ligation of left anterior descending coronary artery (LAD) in male Wistar rats. Rats with myocardial infarction were treated with GXD (1.14 g/kg, 4.53 g/kg) daily for 4 weeks. Cardiac function was evaluated by echocardiography. Hemodynamic parameters and infarct size were measured in each group. Myocardial enzymes were examined by biochemical tests. Inflammatory cytokines were assessed by ELISA, and interrelated proteins were detected by western blot.ResultsCardiac function was significantly improved in GXD-treatment rats after myocardial infarction (MI), which was accompanied with decreased infarct size. Administration of GXD to myocardial fibrosis rats significantly ameliorated the activities of AST, LDH and CK-MB in serum. The increase in inflammatory factors (TNF-α, IL-1β) were markedly reduced upon GXD treatment. Furthermore, the inflammatory mediators (NF-κB p65, TNF-α, MCP-1) were down-regulated by GXD in the myocardial fibrosis rats.ConclusionsTreatment with GXD improved cardiac function induced by myocardial fibrosis by inhibiting expression of inflammatory mediators associated with NF-κB.

show abstract

Section: Methodsmentioning

confidence: 99%

Gualou Xiebai decoction inhibits cardiac dysfunction and inflammation in cardiac fibrosis rats

Ding

Peng

Shen

et al. 2015

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…All animals were subjected to an experimental myocardial infarct (20) by means of occlusion of the anterior descending coronary artery (ADA), for a period of 30 min, followed by reperfusion (open artery).…”

Section: Methodsmentioning

confidence: 99%

High plasma concentrations of asymmetric dimethylarginine inhibit ischemic cardioprotection in hypercholesterolemic rats

Landim

Dourado

Casella-Filho

et al. 2013

Braz J Med Biol Res

View full text Add to dashboard Cite

A low concentration of nitric oxide associated with a high concentration of asymmetric dimethylarginine (ADMA) can explain the lack of ischemic cardioprotection observed in the presence of hypercholesterolemia. The objective of the present study was to evaluate the effect of hypercholesterolemia on ischemic pre- and postconditioning and its correlation with plasma concentrations of ADMA. Male Wistar rats (6-8 weeks old) fed a 2% cholesterol diet (n = 21) for 8 weeks were compared to controls (n = 25) and were subjected to experimental myocardial infarction and reperfusion, with ischemic pre- and postconditioning. Total cholesterol and ADMA were measured in plasma before the experimental infarct and the infarct area was quantified. Weight, total cholesterol and plasma ADMA (means ± SE; 1.20 ± 0.06, 1.27 ± 0.08 and 1.20 ± 0.08 vs 0.97 ± 0.04, 0.93 ± 0.05 and 0.97 ± 0.04 µM) were higher in animals on the hypercholesterolemic diet than in controls, respectively. Cardioprotection did not reduce infarct size in the hypercholesterolemic animals (pre: 13.55% and post: 8% compared to 7.95% observed in the group subjected only to ischemia and reperfusion), whereas infarct size was reduced in the animals on a normocholesterolemic diet (pre: 8.25% and post: 6.10% compared to 12.31%). Hypercholesterolemia elevated ADMA and eliminated the cardioprotective effects of ischemic pre- and postconditioning in rats.

show abstract

“…Esse intervalo é chamado de "limite de concordância". Se a faixa de variação desse intervalo for grande, conclui-se que não há concordância entre os dois métodos (Minicucci et al, 2007).…”

Section: Análise Dos Dados Da Frequência Cardíacaunclassified

Monitoramento da taxa de saturação de oxigênio no sangue e frequência cardíaca via método de magnificação Euleriana sem contato físico

Rosa

Betini

2020

RBCA

View full text Add to dashboard Cite

O monitoramento da frequência cardíaca (FC) e da taxa de saturação de oxigênio no sangue (SpO2) é fundamental em cirurgias e unidades de terapia intensiva. Nas últimas décadas surgiram vários estudos sobre monitoramento de sinais vitais sem contato físico, mas poucos são focados na SpO2. Este artigo apresenta uma solução para o monitoramento da taxa de saturação de oxigênio no sangue e frequência cardíaca sem contato físico com o paciente através da técnica de fotopletismografia por imagens. A magnificação de vídeo Euleriana (EVM) foi empregada na solução proposta para ampliar as variações de cores que ocorrem na pele devido ao ciclo circadiano. O software desenvolvido foi executado em uma plataforma de hardware livre e de baixo custo.Foram realizados experimentos com nove indivíduos saudáveis. Ao comparar os resultados experimentais com os obtidos com um oxímetro de pulso de referência, a solução proposta mostrou boa acurácia nas medidas de SpO2, com diferença menor que 2%, atendendo os requisitos da norma que regulamenta os equipamentos de oximetria de pulso.

show abstract

Comparação de diferentes métodos para medida do tamanho do infarto experimental crônico em Ratos

Abstract: SummaryObjective: To evaluate the differences between three methods for the measurement of experimental infarction in rats in comparison to the traditional method. Methods

Cited by 11 publications

References 18 publications

Gualou Xiebai decoction inhibits cardiac dysfunction and inflammation in cardiac fibrosis rats

Gualou Xiebai decoction inhibits cardiac dysfunction and inflammation in cardiac fibrosis rats

High plasma concentrations of asymmetric dimethylarginine inhibit ischemic cardioprotection in hypercholesterolemic rats

Monitoramento da taxa de saturação de oxigênio no sangue e frequência cardíaca via método de magnificação Euleriana sem contato físico

Contact Info

Product

Resources

About