Gualou Xiebai decoction inhibits cardiac dysfunction and inflammation in cardiac fibrosis rats

Ding, Yongfang; Peng, Yunru; Shen, Hong; Shu, Luan; Wei, Yingjie

doi:10.1186/s12906-016-1012-5

Cited by 17 publications

(12 citation statements)

References 23 publications

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“…The result is similar to the previous research in normal animals. On the other hand, we did not find any change in heart rate in rats after myocardial injury by I/R challenge, nor effect of GLXB on the heart rate either, which is inconsistent with the results by Ding and colleagues reporting that a deceased heart rate was observed in rats with myocardial infarction caused by ligation and subsequent release of the LADCA, while pretreatment with GLXB for 4 weeks prevented the decrease in the heart rate (Ding et al, 2016 ). A possible reason for this difference may be that arrhythmia occurs mostly in the early stage of reperfusion (Sun et al, 2014 ), while our study detected the heart rate at 90 min after reperfusion, missing the time window for finding the effect of GLXB on heart rate.…”

Section: Discussioncontrasting

confidence: 99%

“…Most study regarding the mechanism for the protection of GLXB on I/R-induced myocardial infarction has focused on its effect on apoptosis, oxidative stress and inflammation, and the signaling pathways proposed include NF-κB, TGF-β1, and MAPKs (Ding et al, 2013 , 2016 ). The new finding of the present study is that GLXB increases ATP content by up-regulating the expression of ATP 5D of ATP synthase in the myocardium, suggesting that GLXB may improve energy metabolism.…”

Section: Discussionmentioning

confidence: 99%

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Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury of Hyperlipidemia Rat via Energy Modulation

et al. 2018

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Background: Gualou Xiebai Decoction (GLXB) is a classic prescription of Chinese medicine used for the treatment of cardiac problems. The present study was designed to explore the effect and mechanism of GLXB on ischemia/reperfusion (I/R) induced disorders in myocardial structure and function, focusing on the regulation of energy metabolism and the RhoA/ROCK pathway.Methods: After hyperlipidemic rat model was established by oral administration of high fat diet, the rats were treated with GLXB for 6 weeks and subjected to 30 min occlusion of the left anterior descending coronary artery (LADCA) followed by 90 min reperfusion to elicit I/R challenge. Myocardial infarct size was assessed by Evans blue-TTC staining. Myocardial blood flow (MBF) and cardiac function were evaluated. Enzyme-linked immunosorbent assay was performed to examine the content of ATP, ADP, AMP, CK, CK-MB, LDH, cTnT, cTnI, and IL-6. Double staining of F-actin and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling was conducted to assess myocardial apoptosis. Expressions of ATP synthase subunit δ (ATP 5D), and RhoA and ROCK were determined by Western blotting.Results: Administration with GLXB at high dose for 6 weeks protected heart against I/R-induced MBF decrease, myocardial infarction and apoptosis, ameliorated I/R-caused impairment of cardiac function and myocardial structure, restored the decrease in the ratio of ADP/ATP and AMP/ATP, and the expression of ATP 5D with inhibiting the expression of RhoA and ROCK.Conclusions: Treatment with GLXB effectively protects myocardial structure and function from I/R challenge, possibly via regulating energy metabolism involving inactivation of RhoA/ROCK signaling pathway.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury of Hyperlipidemia Rat via Energy Modulation

et al. 2018

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“…TCM is also used to inhibit myocardial fibrosis by inhibiting expression of inflammatory mediators associated with NF-kB signaling thereby blocking the upregulation of pro-inflammatory cytokines (IL-1β, TNFα) and chemokine (MCP-1), macrophage infiltration and inactivating TGF-β/Smad3 signaling. These actions suppress fibrosis including expression of fibronectin, collagen I and collagen IV[25,26]. TCM’s mechanisms of action differ greatly from that of Western medicine.…”

Section: Discussionmentioning

confidence: 99%

Modified Cortex Mori Capsules improving the successful rate of functional filtering blebs after reclinical glaucoma filtering surgery

Qiu

Qing

et al. 2019

WJCC

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BACKGROUNDThe major reason for filtering bleb failure or scarring of the bleb site is due to excessive scarring after glaucoma filtration surgery in the clinic. Traditional Chinese medicine has preeminence in the prevention of fibrosis formation through the regulation of systemic circulation and improvement of the properties of the inflammatory cells in the blood.AIMTo examine the clinical efficacy of using the Modified Cortex Mori Capsules (MCMC; Chinese name: Jiawei Sangbaipi Capsules) in the success rate of functional filtering blebs after glaucoma filtering surgery in clinical patients.METHODSSixty resurgery glaucoma patients were randomly divided into two groups: 30 patients in surgery with the placebo group and 30 patients in surgery with the MCMC group. Patients took either the placebo or the MCMC 2 wk before and after surgery. Postoperative morphology and function filtering bleb, visual acuity, intraocular pressure, postoperative complications, the success rate of filtration surgery and clinical efficacy were observed.RESULTSFifty patients completed the study. The percentage of functional filtering blebs in the surgery plus MCMC group was 84% at 6 mo after surgery, which was higher than surgery plus placebo group (64%, P < 0.05). The surgical success rate in the MCMC and placebo groups were 79% ± 8.3% and 57% ± 10.6% respectively (P < 0.05). The visual acuity, intraocular pressure and the postoperative complications in the two groups had no significant differences.CONCLUSIONGlaucoma filtering surgery while taking MCMC not only reduced excessive scar formation and increased the success rate of functional filtering blebs but also improved the success of glaucoma filtration operations.

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“…GLXB herb pair consists of the dried fruit of Trichosanthes kirilowii Maxim (Chinese name Gualou) and the dried bulbs of Allium macrostemon Bunge (Chinese name Xiebai) in a weight ratio of 2:1 ( Ding et al, 2016 ). The combination use of Gualou and Xiebai with the ratio of 2:1 was first proposed by Zhang Zhongjing (in the Eastern Han Dynasty, third century China) in the treatise “Jin Kui Yao Lue,” which was verified as the best ratio after a long period of clinical application ( Zhang W. Y. et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Deciphering the combination mechanisms of Gualou–Xiebai herb pair against atherosclerosis by network pharmacology and HPLC-Q-TOF-MS technology

Liu

Zhong

et al. 2022

Front. Pharmacol.

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Introduction: Gualou (Trichosanthes kirilowii Maxim)–Xiebai (Allium macrostemon Bunge) (GLXB) is a well-known herb pair against atherosclerosis (AS). However, the combination mechanisms of GLXB herb pair against AS remain unclear.Objective: To compare the difference in efficacy between GLXB herb pair and the single herbs and to explore the combination mechanisms of GLXB against AS in terms of compounds, targets, and signaling pathways.Methods: The combined effects of GLXB were evaluated in AS mice. The main compounds of GLXB were identified via quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS) and UNIFI informatics platforms. The united mechanisms of GLXB in terms of nodes, key interactions, and functional clusters were realized by network pharmacology. At last, the anti-atherosclerotic mechanisms of GLXB were validated using enzyme-linked immunosorbent assay (ELISA) and Western blot in AS mice.Results: The anti-atherosclerotic effects of the GLXB herb pair (6 g/kg) were more significant than those of Gualou (4 g/kg) and Xiebai (2 g/kg) alone. From the GLXB herb pair, 48 main components were identified. In addition, the GLXB herb pair handled more anti-atherosclerotic targets and more signaling pathways than Gualou or Xiebai alone, whereas 10 key targets of GLXB were found using topological analysis. Furthermore, the GLXB herb pair (6 g/kg) could suppress the inflammatory target levels of IL-6, IL-1β, TNF-α, ALOX5, PTGS2, and p-p38 in AS mice. GLXB herb pair (6 g/kg) could also ameliorate endothelial growth and function by regulating the levels of VEGFA, eNOS, p-AKT, VCAM-1, and ICAM-1 and reducing macrophage adhesion to vascular wall in AS mice. GLXB herb pair (6 g/kg) could improve the blood lipid levels in AS mice. In addition, the regulating effects of GLXB herb pair (6 g/kg) on levels of IL-1β, TNF-α, ALOX5, VEGFA, eNOS, VCAM-1, ICAM-1, and blood lipids were more significant than those of Gualou (4 g/kg) or Xiebai alone (2 g/kg).Conclusion: The combination mechanisms of the GLXB herb pair were elucidated in terms of components, targets, and signaling pathways, which may be related to suppressing inflammation, regulating vascular endothelial growth/function, and improving blood lipid levels.

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Gualou Xiebai decoction inhibits cardiac dysfunction and inflammation in cardiac fibrosis rats

Cited by 17 publications

References 23 publications

Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury of Hyperlipidemia Rat via Energy Modulation

Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury of Hyperlipidemia Rat via Energy Modulation

Modified Cortex Mori Capsules improving the successful rate of functional filtering blebs after reclinical glaucoma filtering surgery

Deciphering the combination mechanisms of Gualou–Xiebai herb pair against atherosclerosis by network pharmacology and HPLC-Q-TOF-MS technology

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