Avaliação do sistema API20C AUX na identificação de leveduras de interesse clínico

Silva, Jaqueline Otero; Candido, Renato

doi:10.1590/s0037-86822005000300012

Cited by 10 publications

(6 citation statements)

References 7 publications

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“…After incubation at 30 ± 2°C, the growth in each well was recorded for 24-72 h. A profile number based upon the reactions was generated for each sample. Identification was performed using the Analytical Profile Index (Gündes et al 2001;Silva and Candido 2005).…”

Section: Microbiological Analysismentioning

confidence: 99%

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Isolation of clinically relevant fungal species from solid waste and environment of dental health services

Vieira

Carvalho

Resende

et al. 2010

Letters in Applied Microbiology

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Aims: This study was undertaken to detect, identify and determine antifungal susceptibility of yeast strains isolated from dental solid waste and to evaluate airborne fungi in the Brazilian dental health care environment and in the waste storage room. Methods and Results: A group of 17 yeast strains were identified by macroscopic and microscopic characteristics, API 20C Aux system and Multiplex PCR. All 104 airborne fungal colonies were identified by macroscopic and microscopic morphology. The CLSI broth microdilution method was utilized as the susceptibility test. Candida parapsilosis was the prevailing yeast species recovered from waste, followed by Rhodotorula glutinis. Three strains of Candida guilliermondii presented minimal inhibitory concentration values considered to be susceptible dose dependent (2 μg ml−1) to voriconazole. Of all airborne fungal species, 69% were recovered from the waste storage room and 31% were recovered from the clinical/surgical environment. Most of them were identified as Cladosporium spp. Conclusions: These findings reinforce the potential risk of waste handling and point out the need for safe management to minimize the spread of these agents to the environment. Filamentous fungi isolation in almost all sampled environments indicates that a periodic monitoring of airborne microbiota in the dental health care service environment is required. Significance and Impact of the Study: The survival of yeast strains for 48 h suggests that dental waste should be carefully controlled and monitored.

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Section: Microbiological Analysismentioning

confidence: 99%

“…Identification was performed using the Analytical Profile Index (Gündes et al. 2001; Silva and Candido 2005).…”

mentioning

confidence: 99%

Isolation of clinically relevant fungal species from solid waste and environment of dental health services

Vieira

Carvalho

Resende

et al. 2010

Letters in Applied Microbiology

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“…Guamán-Burneo & Carvajal-Barriga (2009), while working with different yeasts species of the genus Rhodotorula, found different salmon and pink hues for R. mucilaginosa cultured in CPM agar for 72 h at 20, 30, and 37 °C. Silva & Candido (2005), while working with the same yeast identification system, showed that the API 20C AUX system correctly identified 92% of the 50 yeast species tested.…”

Section: Yeast Identificationmentioning

confidence: 97%

“…The downside of these techniques is the effort to extract and purify the DNA. On the other hand, a simple method based on the assimilative ability of substrates known as API 20C AUX has been widely used to identify yeasts (Silva & Candido, 2005;Chen et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Identifying yeast isolated from spoiled peach puree and assessment of its batch culture for invertase production

Ferreira

Avila²,

Toralles³

et al. 2016

Food Sci. Technol

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The identification of yeasts isolated from spoiled Jubileu peach puree using the API 20C AUX method and a commercial yeast as witness were studied. Subsequently, the yeast's growth potential using two batch culture treatments were performed to evaluate number of colonies (N), reducing sugar concentration (RS), free-invertase (FI), and culture-invertase activity (CI). Stock cultures were maintained on potato dextrose agar (PDA) slants at 4 °C and pH 5 for later use for batch-culture (150 rpm) at 30°C for 24 h, then they were stored at 4 °C for subsequent invertase extraction. The FI extract was obtained using NaHCO 3 as autolysis agent, and CI activity was determined on the supernatant after batch-cultured centrifugation. The activity was followed by an increase in absorbance at 490 nm using the acid 3,5-DNS method with glucose standard. Of the four yeasts identified, Saccharomyces cerevisiae was chosen for legal reasons. It showed logarithmic growth up to 18 h of fermentation with positive correlation CI activity and inverse with RS. FI showed greater activity by the end of the log phase and an inverse correlation with CI activity. Finally, it was concluded that treatment "A" is more effective than "B" to produce invertase (EC 3.2.1.26).Keywords: spoiled peach puree; invertase; Saccharomyces cerevisiae; autolysis.Pratical Application: Potential production of glucose and fructose syrup by yeast invertases from spoiled peach pure.

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“…Essa população da "nova era", ao mesmo tempo, que atinge maior longevidade, também está mais sujeita à infecções oportunísticas por patógenos emergentes. Entre esses agentes microbianos devem ser destacadas espécies do gênero Trichosporon, identificadas como importantes causas de infecção invasiva (GIUSIANO et al, 2004;CANDIDO, 2005;GIRMENIA et al, 2005).…”

Section: Patógenos Emergentesunclassified

Leveduras do gênero Trichosporon: aspectos ecológicos, caracterização laboratorial, fatores associados à virulência e suscetibilidade a antifúngicos.

Bentubo¹

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Avaliação do sistema API20C AUX na identificação de leveduras de interesse clínico

Abstract: RESUMO

Cited by 10 publications

References 7 publications

Isolation of clinically relevant fungal species from solid waste and environment of dental health services

Isolation of clinically relevant fungal species from solid waste and environment of dental health services

Identifying yeast isolated from spoiled peach puree and assessment of its batch culture for invertase production

Leveduras do gênero Trichosporon: aspectos ecológicos, caracterização laboratorial, fatores associados à virulência e suscetibilidade a antifúngicos.

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