Aspectos sorológicos e epidemiológicos da leishmaniose tegumentar americana canina em Maricá, Rio de Janeiro, Brasil

Uchôa, Cláudia Maria Antunes; Serra, Carlos Henrique Aguiar; Duarte, Rosemere; Magalhães, Ciléia M.; Silva, Roger Macedo da; Theophilo, Frederico; Figliuolo, Leticia P.; Horta, Fabiano T.; Madeira, Maria de Fátima

doi:10.1590/s0037-86822001000600011

Cited by 17 publications

(13 citation statements)

References 11 publications

(6 reference statements)

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“…However, variation in the prevalence rate is very high (2.16 to 54.7%) when considering the values estimated by other authors (Pereira et al, 2008;Reis et al, 2011;Araújo et al, 2014;Carvalho et al, 2015;Leça-Júnior et al, 2015). Although IFAs are widely employed for their convenience and low cost, their use is controversial owing to low levels of detectable serum antibodies and the possibility of cross-reactions (Antunes Uchôa et al, 2001). Serological diagnosis results vary depending on the stage of infection (Heusser Júnior et al, 2010).…”

Section: Discussionmentioning

confidence: 93%

“…Serological diagnosis results vary depending on the stage of infection (Heusser Júnior et al, 2010). According to Antunes Uchôa et al (2001), higher levels of specific antibodies are produced when multiple cutaneous lesions are present in the later stages of infection, and in cases with simple skin lesions and lymphatic system involvement. In addition, seroconversion may take up to 22 months; therefore, during this period, it is not possible to detect infected animals by serological methods (Solano-Gallego et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

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Diagnosis and epidemiology of canine leishmaniasis in southeastern Bahia, Brazil

et al. 2016

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ABSTRACT. Leishmaniasis is a disease caused by protozoa of the genus Leishmania. Two distinct forms are recognized: visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL). In the Americas, the causative agent of VL is L. infantum chagasi, whereas L. braziliensis is principally responsible for CL. Domestic dogs constitute the main source of VL in urban environments, and have also been implicated in CL epidemiology. We carried out molecular and serological surveys to detect Leishmania infection in dogs from the municipality of Ituberá in Bahia, Brazil. Furthermore, we identified risk factors associated with illness in dogs from this locality. Blood samples were collected from 399 dogs and tested using an indirect immunofluorescence assay (IFA) and polymerase chain reaction (PCR) to detect Leishmania spp antibodies and L. infantum chagasi and L. braziliensis DNA, respectively. Dogs were clinically evaluated and tissue samples from those exhibiting skin lesions were examined for parasites. In addition, the dog owners completed an epidemiological questionnaire to identify factors associated with infection. Skin lesions consistent with CL were found on 37 (9.3%) of the evaluated animals, but parasitological examination was negative for all samples. The IFA returned positive results for 60 (15%) dogs. PCR identified DNA from L. braziliensis in 86 (21.6%) animals, where as all samples proved negative for L. infantum chagasi. The 134 dogs (33.6%) testing positive using IFA and/ or PCR were considered infected, and of these, only 13 demonstrated skin lesions. Animals from rural areas were 3.39-times more likely to be infected compared to those in urban environments.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Diagnosis and epidemiology of canine leishmaniasis in southeastern Bahia, Brazil

et al. 2016

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“…Uchôa et al (2001) suggested that serological techniques must be used in combination with other diagnostic tests, such as direct PCR-based parasitological exams or the rapid immunochromatographic test.…”

Section: Discussionmentioning

confidence: 99%

Antibodies to Leishmania spp. in domestic felines

Oliveira

Paiz

Menozzi

et al. 2015

Rev. Bras. Parasitol. Vet.

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Leishmaniasis is a vector-borne zoonotic disease caused by protozoa in the genus Leishmania, typical of rural and peri-urban environments. The causative agent of American visceral leishmaniasis is Leishmania (Leishmania) infantum chagasi and the main insect vector in Brazil is Lutzomyia longipalpis. Dogs (Canis familiaris) are important in the transmission of the disease, as a reservoir closely related to humans and an infection source for phlebotomine vectors. Since 1990, an increasing number of feline leishmaniasis cases have been reported, suggesting that domestic cats (Felis catus) might be involved in the epidemiology of the disease. The present study analyzed the prevalence of anti-Leishmania spp. antibodies in naturally infected domestic cats from various neighborhoods in the municipality of Belém, Pará, Brazil, using the indirect immunofluorescence assay (IFA) and the direct agglutination test (DAT). Among the 443 samples tested, 18 (4.06%) presented positive reactions in the IFA. The observed titers were 40 IU in 4.97% of the samples and 80 IU in 0.90%. In the DAT test, positive results were found in 25 (5.64%) of the samples. The observed titers were also 40 IU (4.97%) and 80 IU (0.68%). The agreement rate between the two tests was considered low (Kappa coefficient = 0.10).Keywords: Indirect immunofluorescence assay, direct agglutination test, leishmaniasis, cats. ResumoAs leishmanioses são zoonoses vetoriais causadas por protozoários do gênero Leishmania, características de ambientes rurais e periurbanos. A leishmaniose visceral americana (LVA) é causada pela Leishmania (Leishmania) infantum chagasi, cujo principal vetor no Brasil é Lutzomyia longipalpis. O cão (Canis familiaris) possui papel ativo na transmissão da doença, pois é um reservatório muito próximo do humano e uma fonte de infecção para o flebotomíneo. O aumento do número de casos de leishmaniose felina, descritos na literatura a partir de 1990, sugere Leishmaniasis is a vector-borne zoonotic disease caused by protozoa of the genus Leishmania, Kinetoplastida order, Trypanosomatidae family, and transmitted by phlebotomine species, causing tegumental and visceral injuries (ROSA, 2009). In the parasitic life cycle, promastigotes are found in the vector and amastigotes are found in the infected host tissues (BANETH, 2006). Leishmaniasis is typical of rural and peri-urban environments. Domestic and wild mammals can be infected; humans are considered accidental hosts of the protozoa (CURI et al., 2006).The dog (Canis familiaris) has an active role in the transmission of the disease as an asymptomatic reservoir that is closely related to humans and an infection source to phlebotomine vectors (COURTENAY et al., 2002).American visceral leishmaniasis is caused by Leishmania (Leishmania) infantum chagasi, and is a serious public health problem in Brazil owing to the wide geographical distribution v. 24, n. 4, out.-dez. 2015 465 Leishmania spp. in cats of the disease, high incidence, and the ability to lead to a severe clinical form that ...

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“…No entanto, quando aplicado em estudos epidemiológicos na LTA, a especificidade do ELISA pode tornar-se baixa, devido a ocorrência de reações inespecíficas 17 .…”

Section: Methodsunclassified

Leishmania (Viannia) braziliensis em cães naturalmente infectados

Madeira

Uchôa

Leal

et al. 2003

Rev. Soc. Bras. Med. Trop.

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Resumo Foram estudados oito cães provenientes do Município de Maricá (RJ), com lesões sugestivas de leishmaniose tegumentar americana por métodos parasitológicos e sorológicos. Leishmania spp foi encontrada em seis cães através do cultivo in vitro. Anticorpos específicos foram detectados em seis animais pelo ELISA e em dois pela imunofluorescência indireta. Cinco isolados caninos analisados apresentaram zimodema similar a Leishmania (Viannia) braziliensis. Sugere-se que cães clinicamente suspeitos sejam acompanhados periodicamente, na tentativa de confirmar o diagnóstico da leishmaniose tegumentar canina. Palavras-chaves: Cão. Leishmania. Diagnóstico.Abstract Eight dogs from Maricá Municipality (RJ), with suggestive lesion of american tegumentary leishmaniasis were studied by parasitological and serological methods. Leishmania spp was found in six dogs by in vitro cultivation. Specific antibodies were detected in six dogs by ELISA and in two by indirect immunofluorescence. Five canine isolates were found to belong to the same zymodeme as Leishmania (Viannia) braziliensis. The authors suggest that clinically suspect dogs should be followed-up in an attempt to confirm the diagnostic of canine tegumentary leishmaniasis. Key-words: Dog. Leishmania. Diagnosis. Apesar de ser uma zoonose originalmente silvestre, a LTA causada pela Leishmania (Viannia) braziliensis tem sido descrita por diversos autores ocorrendo em ambientes domésticos, tendo sido aventada a possibilidade de que animais domésticos e peridomésticos, e em especial o cão, estariam funcionando como importantes fontes de infecção nesse locais 2 8 13 . Vários questionamentos ainda permanecem quanto ao verdadeiro papel do cão nessa nova adaptação do ciclo de transmissão 16 .Pouco também se conhece sobre os aspectos clínicos, parasitológicos e imunológicos do curso da infecção pela L. braziliensis em cães, sendo portanto, necessários mais estudos visando elucidar esses aspectos, que contribuirão não só para o diagnóstico precoce dos casos caninos bem como para o esclarecimento do papel dessa espécie animal na epidemiologia da LTA.O presente trabalho teve como objetivo estudar casos de cães com lesão sugestiva de LTA provenientes do Município de Maricá (Rio de Janeiro), descrevendose a apresentação das lesões e correlacionando-as com os resultados parasitológicos e sorológicos.

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Aspectos sorológicos e epidemiológicos da leishmaniose tegumentar americana canina em Maricá, Rio de Janeiro, Brasil

Cited by 17 publications

References 11 publications

Diagnosis and epidemiology of canine leishmaniasis in southeastern Bahia, Brazil

Diagnosis and epidemiology of canine leishmaniasis in southeastern Bahia, Brazil

Antibodies to Leishmania spp. in domestic felines

Leishmania (Viannia) braziliensis em cães naturalmente infectados

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