O real significado de exames sorológicos no diagnóstico de doenças endêmicas

Ar, Teixeira; C, Vexenat Ade

doi:10.1590/s0037-86821996000400013

Cited by 5 publications

(1 citation statement)

References 1 publication

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“…6 Fresh polymerase chain reaction (PCR) is, alone, more sensitive than any other conventional method. 7 Histopathology shows a hystiolymphocitic and plasma cell infiltrate and, rarely, amastigotes.…”

Section: Introductionmentioning

confidence: 99%

Leishmaniose mucosa fatal em criança

Velozo

Cabral

Ribeiro

et al. 2006

An. Bras. Dermatol.

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American cutaneous leishmaniasis, an endemic and increasing disease in Brazil, can present as skin ulcers and lesions in the mucous membranes of the nose, mouth and pharynx. Pentavalent antimony is the first choice drug for treatment, with a less favorable response of the mucosal forms. The difficulty of diagnosing and treating a case of mucosal leishmaniasis in a five-year-old child, with negative parasitological and immunological tests and polymerase chain reaction is emphasized. Only after several attempts was the smear test positive. The patient developed persistent secondary bacterial infection on the lesions, lack of response to specific drugs and antibiotics and evoluted to septicemia and death.

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Section: Introductionmentioning

confidence: 99%

Leishmaniose mucosa fatal em criança

Velozo

Cabral

Ribeiro

et al. 2006

An. Bras. Dermatol.

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Comparative study of amplification systems in immunoenzyme assays for the diagnosis of American tegumentary leishmaniasis

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Passos

Mouta-Confort

et al. 2009

Clinical Laboratory Analysis

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We compared the accuracy and reliability of three amplification systems for enzyme immunoassays in the detection of specific IgG antibodies for the diagnosis of cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis in patients from an endemic area in Rio de Janeiro, Brazil. Partially soluble antigens obtained from the promastigote forms of L. (V.) braziliensis were used. For development of the reaction, two chromogens, 1,2-orthophenylenediamine (OPD) and 3,3',5,5'-tetramethylbenzidine (TMB), and a fluorogen, 4-methylumbelliferylphosphate (MUP), were tested. The performance of each system was compared using the following parameters: accuracy, intraclass correlation coefficient (ICC), and area under the receiver operating characteristic (ROC) curve. Sensitivity was the same (97.4%) for all systems. The reliability was excellent (ICC = 98.6, 98.7, and 99.1%) and specificity was 93.7, 95.8, and 97.4% for OPD, MUP, and TMB, respectively, showing no statistical significance. Despite the absence of differences in the performance of the three systems, the use of TMB is suggested because of its operational advantages, such as low cost compared with fluorogens, easy manipulation, greater stability, and lower toxicity.

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