Antibody subclass profile against Leishmania braziliensis and Leishmania amazonensis in the diagnosis and follow-up of mucosal leishmaniasis

Pedras, Mariana Junqueira; Orsini, Marcela; Castro, Marcelo; Passos, Valéria Maria Azeredo; Rabello, Ana

doi:10.1016/s0732-8893(03)00141-x

Cited by 24 publications

(21 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Currently, serological techniques have increased the sensitivity and/or specificity of the diagnostic tests used for ATL, especially for CL (14,17,18). Earlier work has demonstrated that measurement of IgG subclasses by ELISA or flow cytometry leads to improved diagnosis of ATL (14,18,19). In this study, we have shown that the specificity and sensitivity of the immunoassay are improved by the use of soluble antigens derived by ultracentrifugation of L. braziliensis promastigotes.…”

Section: Discussionmentioning

confidence: 99%

“…The measurement of anti‐ Leishmania IgG1 or IgG3 appears to be a good choice for following up treated CL patients. Although some authors have suggested that the use of species‐specific antigens increases the sensitivity of immunoassays (17,20), others have found that the use of heterologous Leishmania antigens did not result in a reduction in efficacy (14).…”

Section: Discussionmentioning

confidence: 99%

“…Anti‐ Leishmania antibody levels may vary according to parasite load (7), the duration of infection (8), Leishmania species (9,10) and inherent host factors (7). Several authors have positively correlated the clinical cure of ATL with a decline in anti‐ Leishmania immunoglobulins (11–14). The WHO suggests that the clinical criteria of cure of ATL could be supported by serological methods (15).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Decrease in anti‐Leishmania IgG3 and IgG1 after cutaneous leishmaniasis lesion healing is correlated with the time of clinical cure

Fagundes-Silva¹,

Vieira-Gonçalves²,

Nepomuceno³

et al. 2012

Parasite Immunology

View full text Add to dashboard Cite

For better efficiency in the establishment of American tegumentary leishmaniasis clinical cure, the World Health Organization suggests that the clinical criteria are supported by serologic data. The present study aims to investigate the dynamics of IgG subclass production in clinical evolution post-treatment of cutaneous leishmaniasis (CL). Paired sera from 23 subjects with CL resulting from Leishmania braziliensis infection were studied during the active lesion phase (aCL) and after clinical cure post-therapy (hCL), which included an alternative protocol with a low dose of antimony. Anti-Leishmania IgG and its subclasses were measured using ELISA, and the immunoglobulin levels were correlated with patients' clinical data. All of the subjects were clinically healed and did not present relapse during follow-up. Serum levels of anti-Leishmania IgG (r = -0·79; P < 0·0001), IgG1 (r = -0·64, P < 0·001) and IgG3 (r = -0·42, P < 0·045) in hCL were negatively correlated with the duration of clinical cure. After 24 months of clinical cure, 73% of samples were negative for IgG1 and 78% were negative for IgG3. In conclusion, the detection of serum anti-Leishmania IgG1 and IgG3 is an improved laboratory strategy to aid in the decision of interruption of the ambulatory follow-up of CL patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Decrease in anti‐Leishmania IgG3 and IgG1 after cutaneous leishmaniasis lesion healing is correlated with the time of clinical cure

Fagundes-Silva¹,

Vieira-Gonçalves²,

Nepomuceno³

et al. 2012

Parasite Immunology

View full text Add to dashboard Cite

show abstract

“…Several studies have demonstrated that high levels of parasite-specific IgG are generated during leishmaniasis (46,47). This is especially true with human visceral leishmaniasis in which rheumatoid factor (48 -50) and parasite-specific IgG levels are high (27)(28)(29)(30)(31)(32), making it more likely that amastigotes derived from lesions would be opsonized with host IgG. Our model would predict that disease exacerbation caused by immune complexes would only occur late in disease, after parasite-specific IgG was generated.…”

Section: Discussionmentioning

confidence: 99%

“…IgG Abs to Leishmania are prominent in human visceral leishmaniasis, and they are also produced in BALB/c mice during Leishmania infection (27)(28)(29)(30)(31)(32). The levels of IgG increase as disease progresses and parasites disseminate.…”

Section: Il-10 Reduces Ag Presentation and Either Inhibits Or Biases mentioning

confidence: 99%

Activation of the MAPK, ERK, following Leishmania amazonensis Infection of Macrophages

Yang

Mosser

Zhang

2007

The Journal of Immunology

114

124

View full text Add to dashboard Cite

IL-10 is a critical cytokine in determining host susceptibility to Leishmania spp. We previously demonstrated that macrophage-derived IL-10 could contribute to disease exacerbation, but the mechanisms whereby Leishmania infections led to IL-10 induction were not fully understood. In this study, we demonstrated that infection of macrophages with Leishmania amazonensis amastigotes led to the activation of the MAPK, ERK1/2. This activation was required, but not sufficient for IL-10 induction. In addition to ERK activation, an inflammatory stimulus, such as low m.w. hyaluronic acid from the extracellular matrix, must also be present. The combination of these two signals resulted in the superinduction of IL-10. We also demonstrated that IgG on the surface of Leishmania amastigotes was required to achieve maximal IL-10 production from infected macrophages. Surface IgG engages macrophage FcγR to induce ERK activation. Macrophages lacking FcγR, or macrophages treated with an inhibitor of spleen tyrosine kinase, the tyrosine kinase that signals via FcγR, failed to activate ERK and consequently failed to produce IL-10 following infection with Leishmania amastigotes. We confirmed that ERK1/2 activation led to the phosphorylation of histone H3 at the IL-10 promoter, and this phosphorylation allowed for the binding of the transcription factor, Sp1, to the IL-10 promoter. Finally, the administration of U0126, an inhibitor of ERK activation, to infected mice resulted in decreased lesion progression with reduced numbers of parasites in them. Thus, our findings reveal an important role of MAPK, ERK signaling in the pathogenesis of Leishmania infection.

show abstract

Immunoenzymatic assay for the diagnosis of American tegumentary leishmaniasis using soluble and membrane‐enriched fractions from infectious Leishmania (Viannia) braziliensis

Cataldo¹,

Mello²,

Mouta-Confort³

et al. 2010

Clinical Laboratory Analysis

View full text Add to dashboard Cite

The diagnosis of American tegumentary leishmaniasis (ATL) is based on the visualization or isolation of the parasite, which is a time-consuming and poorly sensitive method. In this study, we evaluated the accuracy and reliability of ELISA for the diagnosis of ATL using soluble (SF) and membrane-enriched (MF) antigen fractions obtained from an infectious strain of Leishmania (Viannia) braziliensis. A total of 152 serum samples investigated at a referral center in Rio de Janeiro, Brazil, between 2005 and 2007 were studied. Each sample was tested twice with each fraction for the calculation of reliability (intraclass coefficient (ICC)). Cut-off values of 0.22 (SF) and 0.33 (MF) were defined. The use of the fractions resulted in good discrimination between patients, with a large area under the curve (P<0.0001), but no difference was observed between the two fractions (P=0.45). Sensitivity was 89.5% for each fraction, specificity was 89.5% for SF and 93.4% for MF, and the positive likelihood ratio was 8.5 for SF and 13.6 for MF. The ICCs were excellent (SF: 0.96 and MF: 0.90). The antigens tested provided precision and accuracy for the diagnosis of ATL, with SF being recommended due to its lower cost and greater practicality.

show abstract

Antibody subclass profile against Leishmania braziliensis and Leishmania amazonensis in the diagnosis and follow-up of mucosal leishmaniasis

Cited by 24 publications

References 21 publications

Decrease in anti‐Leishmania IgG3 and IgG1 after cutaneous leishmaniasis lesion healing is correlated with the time of clinical cure

Decrease in anti‐Leishmania IgG3 and IgG1 after cutaneous leishmaniasis lesion healing is correlated with the time of clinical cure

Activation of the MAPK, ERK, following Leishmania amazonensis Infection of Macrophages

Immunoenzymatic assay for the diagnosis of American tegumentary leishmaniasis using soluble and membrane‐enriched fractions from infectious Leishmania (Viannia) braziliensis

Contact Info

Product

Resources

About